Mutants (Isolated)

tm659

Allele Nametm659
Sequence NameC41C4.8
CGC Namecdc-48.2
Worm BaseAllele Name tm659
CGC Name cdc-48.2
Sequence C41C4.8
Phenotypehomozygous viable. Dr. T. Schedl: no germline phenotype, although smaller brood size (180-200/mom). Dr. T. Ogura: BBRC 345, 746-753 (2006). Genes to Cells: 12, 1063 (2007). Dr. A. Colavita: no VC neuron axon branching defects. Dr. T. Hoppe: J. Struct. Biol. 156, 41 (2006), Nat. Cell Biol. 9, 379 (2007).
Mutation site[F10B5] 879/880-[F10B5] 1518/1519 (639 bp deletion)
ChromosomeII
Putative gene structurejoin(39039..39110,39159..39484,39531..39750, Z48334.1:100..855, Z48334.1:908..1626, Z48334.1:1686..2025)
Map position0.71
Balancer
Map position of balancer
Sequence of primersExtFwd:TTTAACCATGGCCTCAGTTC,IntFwd:CCGACTCAGAGAGACGAGAA,ExtRev:TTCAAATCGAGATCGGCGGA,IntRev:GTTTTGCGGAGTGAGGCCTT
Distributed lab
DepositorDr. S. Mitani
References Please submit your publication
Mouysset J, Kähler C, Hoppe T.
A conserved role of Caenorhabditis elegans CDC-48 in ER-associated protein degradation.
J. Struct. Biol. 2006 156(1) 41-9 
[ PubMed ID = 16647269 ] [ RRC reference ]

Yamauchi S, Yamanaka K, Ogura T.
Comparative analysis of expression of two p97 homologues in Caenorhabditis elegans.
Biochem. Biophys. Res. Commun. 2006 345(2) 746-53 
[ PubMed ID = 16701565 ] [ RRC reference ]

Marza E, Taouji S, Barroso K, Raymond AA, Guignard L, Bonneu M, Pallares-Lupon N, Dupuy JW, Fernandez-Zapico ME, Rosenbaum J, Palladino F, Dupuy D, Chevet E.
Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription.
EMBO Rep. 2015 16(3) 332-40 
[ PubMed ID = 25652260 ] [ RRC reference ]

Zou CG, Ma YC, Dai LL, Zhang KQ.
Autophagy protects C. elegans against necrosis during Pseudomonas aeruginosa infection.
Proc. Natl. Acad. Sci. U.S.A. 2014 111(34) 12480-5 
[ PubMed ID = 25114220 ] [ RRC reference ]

Rodrigues AJ, Neves-Carvalho A, Ferro A, Rokka A, Corthals G, Logarinho E, Maciel P.
ATX-3, CDC-48 and UBXN-5: a new trimolecular complex in Caenorhabditis elegans.
Biochem. Biophys. Res. Commun. 2009 386(4) 575-81 
[ PubMed ID = 19545544 ] [ RRC reference ]

Liu G, Rogers J, Murphy CT, Rongo C.
EGF signalling activates the ubiquitin proteasome system to modulate C. elegans lifespan.
EMBO J. 2011 30(15) 2990-3003 
[ PubMed ID = 21673654 ] [ RRC reference ]

Sasagawa Y, Otani M, Higashitani N, Higashitani A, Sato K, Ogura T, Yamanaka K.
Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner.
J. Cell. Sci. 2009 122(Pt 20) 3663-72 
[ PubMed ID = 19773360 ] [ RRC reference ]

Janiesch PC, Kim J, Mouysset J, Barikbin R, Lochmüller H, Cassata G, Krause S, Hoppe T.
The ubiquitin-selective chaperone CDC-48/p97 links myosin assembly to human myopathy.
Nat. Cell Biol. 2007 9(4) 379-90 
[ PubMed ID = 17369820 ] [ RRC reference ]

Sasagawa Y, Yamanaka K, Saito-Sasagawa Y, Ogura T.
Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
Genes Cells 2010 15(12) 1201-15 
[ PubMed ID = 20977550 ] [ RRC reference ]

Arur S, Ohmachi M, Nayak S, Hayes M, Miranda A, Hay A, Golden A, Schedl T.
Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ-line development.
Proc. Natl. Acad. Sci. U.S.A. 2009 106(12) 4776-81 
[ PubMed ID = 19264959 ] [ RRC reference ]

Sasagawa Y, Yamanaka K, Ogura T.
ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans.
Genes Cells 2007 12(9) 1063-73 
[ PubMed ID = 17825049 ] [ RRC reference ]

Kuhlbrodt K, Janiesch PC, Kevei É, Segref A, Barikbin R, Hoppe T.
The Machado-Joseph disease deubiquitylase ATX-3 couples longevity and proteostasis.
Nat. Cell Biol. 2011 13(3) 273-81 
[ PubMed ID = 21317884 ] [ RRC reference ]

Martínez-Velázquez LA, Ringstad N.
Antagonistic regulation of trafficking to Caenorhabditis elegans sensory cilia by a Retinal Degeneration 3 homolog and retromer.
Proc. Natl. Acad. Sci. U.S.A. 2018 115(3) E438-E447 
[ PubMed ID = 29282322 ] [ RRC reference ]

Jablonski AM, Lamitina T, Liachko NF, Sabatella M, Lu J, Zhang L, Ostrow LW, Gupta P, Wu CY, Doshi S, Mojsilovic-Petrovic J, Lans H, Wang J, Kraemer B, Kalb RG.
Loss of RAD-23 Protects Against Models of Motor Neuron Disease by Enhancing Mutant Protein Clearance.
J. Neurosci. 2015 35(42) 14286-306 
[ PubMed ID = 26490867 ] [ RRC reference ]