Mutants (Isolated)

tm5040

Allele Nametm5040
Allele TypeNormal
Sequence NameT27F6.4
Gene NameT27F6.4
Worm BaseAllele Name tm5040
Gene Name T27F6.4
Sequence T27F6.4
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable.
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 13777/13778-AAAATATAAAATATAT-14506/14507 (729 bp deletion + 16 bp insertion)
ChromosomeI
Putative gene structurejoin(14022..14204, 14852..15130, 15804..15962)
Map position13.23
Balancer
Map position of balancer
Sequence of primersIntFwd:CACGCTTTTGCCGTGGGAAT,ExtRev:AAGGGCTTGGGACTGCGAAA,IntRev:GGACTGCGAAAATCAGGCTT,ExtFwd:AGCGCACAGTTTCCCACGCT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Brenner JL, Schedl T.
Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans.
Genetics 2016 202(3) 1085-103 
[ PubMed ID = 26757772 ] [ RRC reference ]

Shin H, Haupt KA, Kershner AM, Kroll-Conner P, Wickens M, Kimble J.
SYGL-1 and LST-1 link niche signaling to PUF RNA repression for stem cell maintenance in Caenorhabditis elegans.
PLoS Genet 2017 13(12) e1007121 
[ PubMed ID = 29232700 ] [ RRC reference ]

Kershner AM, Shin H, Hansen TJ, Kimble J.
Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance.
Proc Natl Acad Sci U S A 2014 111(10) 3739-44 
[ PubMed ID = 24567412 ] [ RRC reference ]