Mutants (Isolated)

tm504

Allele Nametm504
Sequence NameF59G1.1
CGC Namecgt-3
Worm BaseAllele Name tm504
CGC Name cgt-3
Sequence F59G1.1
Phenotypehomozygous viable
Mutation site19145/19146-ACGACACTGATTGCCATCGTTGGCTTCGTCTTCGTATTTTGCCTCTACCTTATTCAC-20195/20196 (1050 bp deletion + 57 bp insertion)
ChromosomeII
Putative gene structurejoin(18128..18158, 18956..19223, 19275..19510, 19565..19785, 19836..20159, 20203..20334, 20383..20706)
Map position-0.86
Balancer
Map position of balancer
Sequence of primersIntFwd:CTTGGCAGGTGTGAACATAG,IntRev:GTTGGAATTTTTCTGAACCTGA,ExtRev:GGTGATATAAATTACATAGTTGGA,ExtFwd:GACGCCTTTTCAGTGCAAAT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Boland S, Schmidt U, Zagoriy V, Sampaio JL, Fritsche RF, Czerwonka R, Lübken T, Reimann J, Penkov S, Knölker HJ, Kurzchalia TV.
Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans.
Nat. Chem. Biol. 2017 13(6) 647-654 
[ PubMed ID = 28369040 ] [ RRC reference ]

Zhu H, Shen H, Sewell AK, Kniazeva M, Han M.
A novel sphingolipid-TORC1 pathway critically promotes postembryonic development in Caenorhabditis elegans.
Elife 2013 2 e00429 
[ PubMed ID = 23705068 ] [ RRC reference ]

Zhang H, Abraham N, Khan LA, Hall DH, Fleming JT, Göbel V.
Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.
Nat. Cell Biol. 2011 13(10) 1189-201 
[ PubMed ID = 21926990 ] [ RRC reference ]

Nomura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K.
Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division.
Glycobiology 2011 21(6) 834-48 
[ PubMed ID = 21325339 ] [ RRC reference ]

Marza E, Simonsen KT, Faergeman NJ, Lesa GM.
Expression of ceramide glucosyltransferases, which are essential for glycosphingolipid synthesis, is only required in a small subset of C. elegans cells.
J. Cell. Sci. 2009 122(Pt 6) 822-33 
[ PubMed ID = 19240113 ] [ RRC reference ]

Kniazeva M, Zhu H, Sewell AK, Han M.
A Lipid-TORC1 Pathway Promotes Neuronal Development and Foraging Behavior under Both Fed and Fasted Conditions in C. elegans.
Dev. Cell 2015 33(3) 260-71 
[ PubMed ID = 25892013 ] [ RRC reference ]

Cui M, Wang Y, Cavaleri J, Kelson T, Teng Y, Han M.
Starvation-Induced Stress Response Is Critically Impacted by Ceramide Levels in Caenorhabditis elegans.
Genetics 2017 205(2) 775-785 
[ PubMed ID = 27974500 ] [ RRC reference ]

Hao L, Ben-David O, Babb SM, Futerman AH, Cohen BM, Buttner EA.
Clozapine Modulates Glucosylceramide, Clears Aggregated Proteins, and Enhances ATG8/LC3 in Caenorhabditis elegans.
Neuropsychopharmacology 2017 42(4) 951-962 
[ PubMed ID = 27711049 ] [ RRC reference ]