Mutants (Isolated)

tm373

Allele Name

tm373

Sequence Name

C48D1.3

CGC Name

cho-1

Worm Base

Allele Name

tm373

CGC Name

cho-1

Sequence

C48D1.3

Phenotype

homozygous viable. Dr. S. McIntire: normal locomotion and egg laying. Dr. J. Rand: moderate aldicarb resistence, slow thrashing, fatiques when swimming.

Mutation site

18992/18993-20687/20688 (1695 bp deletion)

Chromosome

Putative gene structure

complement(join(16940..17126, 18419..18597, 18985..19442, 19553..19879, 19925..19961, 20333..20481, 20789..20987, 21037..21152, 21373..21616))

Map position

8.47

Balancer

Map position of balancer

Sequence of primers

IntFwd:AGGTCTCACTACTAACAGCA,IntRev:ATGGGCGGGTAGAAAATCGA,ExtFwd:GGGGGAATTGCATTCCGTCA,ExtRev:GTGCTCATTCTTGTCGTTGG

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Wang H, Salter CG, Refai O, Hardy H, Barwick KES, Akpulat U, Kvarnung M, Chioza BA, Harlalka G, Taylan F, Sejersen T, Wright J, Zimmerman HH, Karakaya M, Stüve B, Weis J, Schara U, Russell MA, Abdul-Rahman OA, Chilton J, Blakely RD, Baple EL, Cirak S, Crosby AH.
Choline transporter mutations in severe congenital myasthenic syndrome disrupt transporter localization.
Brain 2017 140(11) 2838-2850
[ PubMed ID = 29088354 ] [ RRC reference ]

Wen X, Chen YH, Li R, Ge MH, Yin SW, Wu JJ, Huang JH, Liu H, Wang PZ, Gross E, Wu ZX.
Signal Decoding for Glutamate Modulating Egg Laying Oppositely in Caenorhabditiselegans under Varied Environmental Conditions.
iScience 2020 23(10) 101588
[ PubMed ID = 33089099 ] [ RRC reference ]

Rand JB.
Acetylcholine.
WormBook 2007 1-21
[ PubMed ID = 18050502 ] [ RRC reference ]

Mullen GP, Mathews EA, Vu MH, Hunter JW, Frisby DL, Duke A, Grundahl K, Osborne JD, Crowell JA, Rand JB.
Choline transport and de novo choline synthesis support acetylcholine biosynthesis in Caenorhabditis elegans cholinergic neurons.
Genetics 2007 177(1) 195-204
[ PubMed ID = 17603106 ] [ RRC reference ]

Matthies DS, Fleming PA, Wilkes DM, Blakely RD.
The Caenorhabditis elegans choline transporter CHO-1 sustains acetylcholine synthesis and motor function in an activity-dependent manner.
J Neurosci 2006 26(23) 6200-12
[ PubMed ID = 16763028 ] [ RRC reference ]

Kim H, Rogers MJ, Richmond JE, McIntire SL.
SNF-6 is an acetylcholine transporter interacting with the dystrophin complex in Caenorhabditis elegans.
Nature 2004 430(7002) 891-6
[ PubMed ID = 15318222 ] [ RRC reference ]