Mutants (Isolated)

tm3425

Allele Nametm3425
Sequence NameC56C10.12
CGC Nameepg-5
Worm BaseAllele Name tm3425
CGC Name epg-5
Sequence C56C10.12
Phenotypehomozygous viable. Dr. S. McIntire: sluggish locomotion. Dr. H. Sawa: not Psa, vacuoles in the gut.
Mutation site33713/33714-34452/34453 (739 bp deletion)
ChromosomeII
Putative gene structurecomplement(join(29155..29274, 29369..29455, 29504..29960, 30356..31552, 31600..32324, 32368..32492, 32540..32832, 32879..32955, 33005..33063, 33119..33506, 33558..33691, 33742..34345, 34392..34649, 34696..34795, 34845..34906, 34957..35070))
Map position-0.08
Balancer
Map position of balancer
Sequence of primersIntRev:TGGCGGAATTGGTTCGTCCA,ExtFwd:CGAACGCTGTGCCTGAAAGA,IntFwd:TCTAGGAGCCAAAGGATAAC,ExtRev:TCGTACTGTACTTGTCGCAT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
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Tian Y, Li Z, Hu W, Ren H, Tian E, Zhao Y, Lu Q, Huang X, Yang P, Li X, Wang X, Kovács AL, Yu L, Zhang H.
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Cell 2010 141(6) 1042-55 
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Sun T, Wang X, Lu Q, Ren H, Zhang H.
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Autophagy 2011 7(11) 1308-15 
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Autophagy 2013 9(2) 138-49 
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Lu Q, Yang P, Huang X, Hu W, Guo B, Wu F, Lin L, Kovács AL, Yu L, Zhang H.
The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of omegasomes to autophagosomes.
Dev. Cell 2011 21(2) 343-57 
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Zhang P, Zhang H.
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Wang H, Lu Q, Cheng S, Wang X, Zhang H.
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Min H, Lee YU, Shim YH, Kawasaki I.
Autophagy of germ-granule components, PGL-1 and PGL-3, contributes to DNA damage-induced germ cell apoptosis in C. elegans.
PLoS Genet. 2019 15(5) e1008150 
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Lin L, Yang P, Huang X, Zhang H, Lu Q, Zhang H.
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Wu F, Watanabe Y, Guo XY, Qi X, Wang P, Zhao HY, Wang Z, Fujioka Y, Zhang H, Ren JQ, Fang TC, Shen YX, Feng W, Hu JJ, Noda NN, Zhang H.
Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy.
Mol. Cell 2015 60(6) 914-29 
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Cheng S, Wu Y, Lu Q, Yan J, Zhang H, Wang X.
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Autophagy 2013 9(12) 2022-32 
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Guo B, Liang Q, Li L, Hu Z, Wu F, Zhang P, Ma Y, Zhao B, Kovács AL, Zhang Z, Feng D, Chen S, Zhang H.
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Stavoe AK, Hill SE, Hall DH, Colón-Ramos DA.
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Chen Y, Scarcelli V, Legouis R.
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Cells 2017 6(3)  
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