Mutants (Isolated)


Allele Nametm324
Sequence NameF20C5.2
CGC Nameklp-11
Worm BaseAllele Name tm324
CGC Name klp-11
Sequence F20C5.2
Phenotypehomozygous viable; Dr. M.M. Barr: Dyf+, PKD-2::GFP localization: subtle ciliary mislocalization; Dr. P. Sengupta: 10% of animals fail to dye-fill one AWB neuron when grwon at 25C. Other neurons dye-fill normally. Dr. K. Shen: normal synapse formation as determined by RAB-3 and CCB-1 localization to presynaptic sites in HSNL. Dr. C. Bargmann: locomotion OK. Dr. H. Sawa: 0% Psa.
Mutation site15970/15971-16332/16333 (362 bp deletion)
Putative gene structurecomplement(join(12579..12756, 12832..13218, 13268..13435, 13540..13599, 13738..13934, 13984..14106, 14390..14524, 14807..14952, 15446..15548, 16119..16234, 16325..16467, 16686..16783,16835..17431, 17474..17795, 17847..17917, 17970..18062, 18106..18355, 18495..18599, 18649..18704, 19251..19295)
Map position3.96
Map position of balancer
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Xie C, Li L, Li M, Shao W, Zuo Q, Huang X, Chen R, Li W, Brunnbauer M, Ökten Z, Chen L, Ou G.
Optimal sidestepping of intraflagellar transport kinesins regulates structure and function of sensory cilia.
EMBO J 2020 39(12) e103955 
[ PubMed ID = 32338401 ] [ RRC reference ]

Niwa S.
The nephronophthisis-related gene ift-139 is required for ciliogenesis in Caenorhabditis elegans.
Sci Rep 2016 6 31544 
[ PubMed ID = 27515926 ] [ RRC reference ]

Pan X, Acar S, Scholey JM.
Torque generation by one of the motor subunits of heterotrimeric kinesin-2.
Biochem Biophys Res Commun 2010 401(1) 53-7 
[ PubMed ID = 20833139 ] [ RRC reference ]

Wang J, Silva M, Haas LA, Morsci NS, Nguyen KC, Hall DH, Barr MM.
C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication.
Curr Biol 2014 24(5) 519-25 
[ PubMed ID = 24530063 ] [ RRC reference ]

Barsi-Rhyne BJ, Miller KM, Vargas CT, Thomas AB, Park J, Bremer M, Jarecki JL, VanHoven MK.
Kinesin-1 acts with netrin and DCC to maintain sensory neuron position in Caenorhabditis elegans.
Genetics 2013 194(1) 175-87 
[ PubMed ID = 23475988 ] [ RRC reference ]

Kazatskaya A, Kuhns S, Lambacher NJ, Kennedy JE, Brear AG, McManus GJ, Sengupta P, Blacque OE.
Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.
Genetics 2017 207(4) 1423-1440 
[ PubMed ID = 29021280 ] [ RRC reference ]

Kaplan OI, Doroquez DB, Cevik S, Bowie RV, Clarke L, Sanders AA, Kida K, Rappoport JZ, Sengupta P, Blacque OE.
Endocytosis genes facilitate protein and membrane transport in C. elegans sensory cilia.
Curr Biol 2012 22(6) 451-60 
[ PubMed ID = 22342749 ] [ RRC reference ]

Sanders AA, de Vrieze E, Alazami AM, Alzahrani F, Malarkey EB, Sorusch N, Tebbe L, Kuhns S, van Dam TJ, Alhashem A, Tabarki B, Lu Q, Lambacher NJ, Kennedy JE, Bowie RV, Hetterschijt L, van Beersum S, van Reeuwijk J, Boldt K, Kremer H, Kesterson RA, Monies D, Abouelhoda M, Roepman R, Huynen MH, Ueffing M, Russell RB, Wolfrum U, Yoder BK, van Wijk E, Alkuraya FS, Blacque OE.
KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome.
Genome Biol 2015 16 293 
[ PubMed ID = 26714646 ] [ RRC reference ]

Hao L, Thein M, Brust-Mascher I, Civelekoglu-Scholey G, Lu Y, Acar S, Prevo B, Shaham S, Scholey JM.
Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments.
Nat Cell Biol 2011 13(7) 790-8 
[ PubMed ID = 21642982 ] [ RRC reference ]

Hao L, Efimenko E, Swoboda P, Scholey JM.
The retrograde IFT machinery of C. elegans cilia: two IFT dynein complexes?
PLoS One 2011 6(6) e20995 
[ PubMed ID = 21695221 ] [ RRC reference ]

Cevik S, Sanders AA, Van Wijk E, Boldt K, Clarke L, van Reeuwijk J, Hori Y, Horn N, Hetterschijt L, Wdowicz A, Mullins A, Kida K, Kaplan OI, van Beersum SE, Man Wu K, Letteboer SJ, Mans DA, Katada T, Kontani K, Ueffing M, Roepman R, Kremer H, Blacque OE.
Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain.
PLoS Genet 2013 9(12) e1003977 
[ PubMed ID = 24339792 ] [ RRC reference ]

O'Hagan R, Silva M, Nguyen KCQ, Zhang W, Bellotti S, Ramadan YH, Hall DH, Barr MM.
Glutamylation Regulates Transport, Specializes Function, and Sculpts the Structure of Cilia.
Curr Biol 2017 27(22) 3430-3441.e6 
[ PubMed ID = 29129530 ] [ RRC reference ]

Li C, Inglis PN, Leitch CC, Efimenko E, Zaghloul NA, Mok CA, Davis EE, Bialas NJ, Healey MP, Héon E, Zhen M, Swoboda P, Katsanis N, Leroux MR.
An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes.
PLoS Genet 2008 4(3) e1000044 
[ PubMed ID = 18369462 ] [ RRC reference ]

Mukhopadhyay S, Lu Y, Qin H, Lanjuin A, Shaham S, Sengupta P.
Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans.
EMBO J 2007 26(12) 2966-80 
[ PubMed ID = 17510633 ] [ RRC reference ]

Pan X, Ou G, Civelekoglu-Scholey G, Blacque OE, Endres NF, Tao L, Mogilner A, Leroux MR, Vale RD, Scholey JM.
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors.
J Cell Biol 2006 174(7) 1035-45 
[ PubMed ID = 17000880 ] [ RRC reference ]

Evans JE, Snow JJ, Gunnarson AL, Ou G, Stahlberg H, McDonald KL, Scholey JM.
Functional modulation of IFT kinesins extends the sensory repertoire of ciliated neurons in Caenorhabditis elegans.
J Cell Biol 2006 172(5) 663-9 
[ PubMed ID = 16492809 ] [ RRC reference ]

Blacque OE, Li C, Inglis PN, Esmail MA, Ou G, Mah AK, Baillie DL, Scholey JM, Leroux MR.
The WD repeat-containing protein IFTA-1 is required for retrograde intraflagellar transport.
Mol Biol Cell 2006 17(12) 5053-62 
[ PubMed ID = 17021254 ] [ RRC reference ]

Qin H, Burnette DT, Bae YK, Forscher P, Barr MM, Rosenbaum JL.
Intraflagellar transport is required for the vectorial movement of TRPV channels in the ciliary membrane.
Curr Biol 2005 15(18) 1695-9 
[ PubMed ID = 16169494 ] [ RRC reference ]

Ou G, Blacque OE, Snow JJ, Leroux MR, Scholey JM.
Functional coordination of intraflagellar transport motors.
Nature 2005 436(7050) 583-7 
[ PubMed ID = 16049494 ] [ RRC reference ]

Mok CA, Healey MP, Shekhar T, Leroux MR, Héon E, Zhen M.
Mutations in a guanylate cyclase GCY-35/GCY-36 modify Bardet-Biedl syndrome-associated phenotypes in Caenorhabditis elegans.
PLoS Genet 2011 7(10) e1002335 
[ PubMed ID = 22022287 ] [ RRC reference ]