Mutants (Isolated)

tm2966

Allele Nametm2966
Allele TypeNormal
Sequence NameY48B6A.11
Gene Namejmjd-2
Worm BaseAllele Name tm2966
Gene Name jmjd-2
Sequence Y48B6A.11
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable. Dr. M. Han: normal vulval phenotype.
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 89670/89671-TNCACACACACCTTAATC-90343/90344 (673 bp deletion + 18 bp insertion)
ChromosomeII
Putative gene structurecomplement(join(86445..87456, 88264..88408, 89561..90651, 91314..91538, 93031..93242))
Map position22.9
Balancer
Map position of balancer
Sequence of primersIntFwd:GGTCTCAGCTGCCAATTGTG,ExtFwd:AAGCTCATCATAATCCGGTC,IntRev:GCCCGGAGCCTACTTCAAAC,ExtRev:CGGCCCGCGAAACAATCGAA
Distributed lab
DepositorDr. S. Mitani
References Please submit your publication
Zaghet N, Madsen K, Rossi F, Perez DF, Amendola PG, Demharter S, Pfisterer U, Khodosevich K, Pasini D, Salcini AE.
Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality.
Cell Rep 2021 37(8) 110050 
[ PubMed ID = 34818537 ] [ RRC reference ]

Mutlu B, Chen HM, Gutnik S, Hall DH, Keppler-Ross S, Mango SE.
Distinct functions and temporal regulation of methylated histone H3 during early embryogenesis.
Development 2019 146(19)  
[ PubMed ID = 31540912 ] [ RRC reference ]

Kreher J, Takasaki T, Cockrum C, Sidoli S, Garcia BA, Jensen ON, Strome S.
Distinct Roles of Two Histone Methyltransferases in Transmitting H3K36me3-Based Epigenetic Memory Across Generations in Caenorhabditis elegans.
Genetics 2018 210(3) 969-982 
[ PubMed ID = 30217796 ] [ RRC reference ]

Greer EL, Becker B, Latza C, Antebi A, Shi Y.
Mutation of C. elegans demethylase spr-5 extends transgenerational longevity.
Cell Res 2016 26(2) 229-38 
[ PubMed ID = 26691751 ] [ RRC reference ]

Black JC, Allen A, Van Rechem C, Forbes E, Longworth M, Tschöp K, Rinehart C, Quiton J, Walsh R, Smallwood A, Dyson NJ, Whetstine JR.
Conserved antagonism between JMJD2A/KDM4A and HP1γ during cell cycle progression.
Mol Cell 2010 40(5) 736-48 
[ PubMed ID = 21145482 ] [ RRC reference ]

Zheng C, Karimzadegan S, Chiang V, Chalfie M.
Histone methylation restrains the expression of subtype-specific genes during terminal neuronal differentiation in Caenorhabditis elegans.
PLoS Genet 2013 9(12) e1004017 
[ PubMed ID = 24348272 ] [ RRC reference ]

Greer EL, Beese-Sims SE, Brookes E, Spadafora R, Zhu Y, Rothbart SB, Aristizábal-Corrales D, Chen S, Badeaux AI, Jin Q, Wang W, Strahl BD, Colaiácovo MP, Shi Y.
A histone methylation network regulates transgenerational epigenetic memory in C. elegans.
Cell Rep 2014 7(1) 113-26 
[ PubMed ID = 24685137 ] [ RRC reference ]