Mutants (Isolated)

tm2543

Allele Name

tm2543

Allele Type

Normal

Sequence Name

Y73B6BL.2

Gene Name

htp-2

Worm Base

Allele Name

tm2543

Gene Name

htp-2

Sequence

Y73B6BL.2

Phenotype

homozygous viable. Dr. A. Dernburg: very weak Him (~1%). Dr. E. Marinez-Perez: Genes & Dev. 22, 2886 (2008).

Mutation site

133607/133608-GGCTGTTCTC-133766/133767 (159 bp deletion + 10 bp insertion)

Chromosome

Putative gene structure

join(133377..133466, 133512..133659, 133706..134439, 134489..134599)

Map position

3.2

Balancer

Map position of balancer

Sequence of primers

IntFwd:GACCCGGATCGATCGTCCAA,ExtFwd:AGCATTGTCAGCGACCCGGA,IntRev:TCGAAGCCATCGATGCGGAA,ExtRev:GGTGACCGATTGGATACCAT

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Russo AE, Giacopazzi S, Deshong A, Menon M, Ortiz V, Ego KM, Corbett KD, Bhalla N.
The conserved AAA ATPase PCH-2 distributes its regulation of meiotic prophase events through multiple meiotic HORMADs in C. elegans.
PLoS Genet 2023 19(4) e1010708
[ PubMed ID = 37058535 ] [ RRC reference ]

Mazzetto M, Gonzalez LE, Sanchez N, Reinke V.
Characterization of the distribution and dynamics of chromatin states in the C. elegans germline reveals substantial H3K4me3 remodeling during oogenesis.
Genome Res 2024 34(1) 57-69
[ PubMed ID = 38164610 ] [ RRC reference ]

Loose JA, Amrit FRG, Patil T, Yanowitz JL, Ghazi A.
Meiotic dysfunction accelerates somatic aging in Caenorhabditis elegans.
Aging Cell 2022 21(11) e13716
[ PubMed ID = 36176234 ] [ RRC reference ]

Woglar A, Yamaya K, Roelens B, Boettiger A, Köhler S, Villeneuve AM.
Quantitative cytogenetics reveals molecular stoichiometry and longitudinal organization of meiotic chromosome axes and loops.
PLoS Biol 2020 18(8) e3000817
[ PubMed ID = 32813728 ] [ RRC reference ]

Ferrandiz N, Barroso C, Telecan O, Shao N, Kim HM, Testori S, Faull P, Cutillas P, Snijders AP, Colaiácovo MP, Martinez-Perez E.
Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis.
Nat Commun 2018 9(1) 834
[ PubMed ID = 29483514 ] [ RRC reference ]

Bohr T, Ashley G, Eggleston E, Firestone K, Bhalla N.
Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans.
Genetics 2016 204(3) 987-997
[ PubMed ID = 27605049 ] [ RRC reference ]

Schvarzstein M, Pattabiraman D, Bembenek JN, Villeneuve AM.
Meiotic HORMA domain proteins prevent untimely centriole disengagement during Caenorhabditis elegans spermatocyte meiosis.
Proc Natl Acad Sci U S A 2013 110(10) E898-907
[ PubMed ID = 23401519 ] [ RRC reference ]

Roelens B, Schvarzstein M, Villeneuve AM.
Manipulation of Karyotype in Caenorhabditis elegans Reveals Multiple Inputs Driving Pairwise Chromosome Synapsis During Meiosis.
Genetics 2015 201(4) 1363-79
[ PubMed ID = 26500263 ] [ RRC reference ]

Kim Y, Kostow N, Dernburg AF.
The Chromosome Axis Mediates Feedback Control of CHK-2 to Ensure Crossover Formation in C. elegans.
Dev Cell 2015 35(2) 247-61
[ PubMed ID = 26506311 ] [ RRC reference ]

Martinez-Perez E, Schvarzstein M, Barroso C, Lightfoot J, Dernburg AF, Villeneuve AM.
Crossovers trigger a remodeling of meiotic chromosome axis composition that is linked to two-step loss of sister chromatid cohesion.
Genes Dev 2008 22(20) 2886-901
[ PubMed ID = 18923085 ] [ RRC reference ]