Mutants (Isolated)

tm2252

Allele Nametm2252
Sequence NameZC477.5
CGC Namerde-8
Worm BaseAllele Name tm2252
CGC Name rde-8
Sequence ZC477.5
Phenotypehomozygous viable. Rde against gon-1 (RNAi).
Mutation site16461/16462-GGTAGAGGTTATGGTGG-17665/17666 (1204 bp deletion + 17 bp insertion)
ChromosomeIV
Putative gene structurecomplement(join(16318..16438, 16971..17053, 17098..17195, 17243..17671, 17720..17825, 17878..17977, 18024..18106))
Map position3.29
Balancer
Map position of balancer
Sequence of primersIntFwd:GGTTGTTGTGTAGAGTGGTC,ExtFwd:CTCAGGTGGCTGTGGTTGTT,IntRev:GATGAATGCGCCAGCAGTGT,ExtRev:CAGATGGACGTACTCACATC
Distributed lab
DepositorDr. S. Mitani
References Please submit your publication
Uebel CJ, Anderson DC, Mandarino LM, Manage KI, Aynaszyan S, Phillips CM.
Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci.
PLoS Genet 2018 14(7) e1007542 
[ PubMed ID = 30036386 ] [ RRC reference ]

Tsai HY, Chen CC, Conte D Jr, Moresco JJ, Chaves DA, Mitani S, Yates JR 3rd, Tsai MD, Mello CC.
A ribonuclease coordinates siRNA amplification and mRNA cleavage during RNAi.
Cell 2015 160(3) 407-19 
[ PubMed ID = 25635455 ] [ RRC reference ]

Kim KW, Tang NH, Andrusiak MG, Wu Z, Chisholm AD, Jin Y.
A Neuronal piRNA Pathway Inhibits Axon Regeneration in C. elegans.
Neuron 2018 97(3) 511-519.e6 
[ PubMed ID = 29395906 ] [ RRC reference ]