Mutants (Isolated)

tm1888

Allele Nametm1888
Sequence NameF13B12.5
CGC Nameins-1
Worm BaseAllele Name tm1888
CGC Name ins-1
Sequence F13B12.5
Phenotypehomozygous viable. Dr. S. Tuck: does not affect rsks-1 gene. L. Avery: intact satiety behavior. Dr. S. Tuck: Cell 128, 577-587 (2007). Dr. Z-X. Wu: normal chemotaxis against glycerol, Cu and normal nose touch response.
Mutation site21217/21218-21965/21966 (748 bp deletion)
ChromosomeIV
Putative gene structurejoin(20800..20825, 20912..21063, 21887..22038)
Map position4.59
Balancer
Map position of balancer
Sequence of primersIntRev:TCCGGTGAGCTGCCAACGAT,ExtRev:GGATGCATCAGACGACTGTG,IntFwd:GCAGACAGGAAATCATCTAC,ExtFwd:GTCCTGACTGCAAACGAGCA
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Dwyer DS, Aamodt EJ.
Insulin/IGF-1 signaling, including class II/III PI3Ks, β-arrestin and SGK-1, is required in C. elegans to maintain pharyngeal muscle performance during starvation.
PLoS ONE 2013 8(5) e63851 
[ PubMed ID = 23700438 ] [ RRC reference ]

Kao G, Nordenson C, Still M, Rönnlund A, Tuck S, Naredi P.
ASNA-1 positively regulates insulin secretion in C. elegans and mammalian cells.
Cell 2007 128(3) 577-87 
[ PubMed ID = 17289575 ] [ RRC reference ]

Harris G, Korchnak A, Summers P, Hapiak V, Law WJ, Stein AM, Komuniecki P, Komuniecki R.
Dissecting the serotonergic food signal stimulating sensory-mediated aversive behavior in C. elegans.
PLoS ONE 2011 6(7) e21897 
[ PubMed ID = 21814562 ] [ RRC reference ]

Ryu L, Cheon Y, Huh YH, Pyo S, Chinta S, Choi H, Butcher RA, Kim K.
Feeding state regulates pheromone-mediated avoidance behavior via the insulin signaling pathway in Caenorhabditis elegans.
EMBO J. 2018 37(15)  
[ PubMed ID = 29925517 ] [ RRC reference ]

Hung WL, Wang Y, Chitturi J, Zhen M.
A Caenorhabditis elegans developmental decision requires insulin signaling-mediated neuron-intestine communication.
Development 2014 141(8) 1767-79 
[ PubMed ID = 24671950 ] [ RRC reference ]

Lee D, Lee H, Kim N, Lim DS, Lee J.
Regulation of a hitchhiking behavior by neuronal insulin and TGF-β signaling in the nematode Caenorhabditis elegans.
Biochem. Biophys. Res. Commun. 2017 484(2) 323-330 
[ PubMed ID = 28131836 ] [ RRC reference ]

Hung WL, Hwang C, Gao S, Liao EH, Chitturi J, Wang Y, Li H, Stigloher C, Bessereau JL, Zhen M.
Attenuation of insulin signalling contributes to FSN-1-mediated regulation of synapse development.
EMBO J. 2013 32(12) 1745-60 
[ PubMed ID = 23665919 ] [ RRC reference ]