Mutants (Isolated)

tm1863

Allele Name

tm1863

Allele Type

Normal

Sequence Name

B0041.2

Gene Name

ain-2

Worm Base

Allele Name

tm1863

Gene Name

ain-2

Sequence

B0041.2

Phenotype

homozygous viable. Dr. M. Han: WT vulval development, WT growth, WT adult alae, WT touch response.

Mutation site

12204/12205-12893/12894 (689 bp deletion)

Chromosome

Putative gene structure

join(12233..12359, 12584..13321, 13374..13643, 14069..14350, 14802..15118, 15548..15625, 15872..16180)

Map position

-1.03

Balancer

Map position of balancer

Sequence of primers

IntRev:CACGTGACGAGCTTTGATCG,ExtFwd:TACTCTGGGTTTCACCTGCT,ExtRev:TCCCAACCACGTGACGAGCT,IntFwd:ACCGTACCCCTACCTTTGCT

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Kogure A, Uno M, Ikeda T, Nishida E.
The microRNA machinery regulates fasting-induced changes in gene expression and longevity in Caenorhabditis elegans.
J Biol Chem 2017 292(27) 11300-11309
[ PubMed ID = 28507100 ] [ RRC reference ]

Kudlow BA, Zhang L, Han M.
Systematic analysis of tissue-restricted miRISCs reveals a broad role for microRNAs in suppressing basal activity of the C. elegans pathogen response.
Mol Cell 2012 46(4) 530-41
[ PubMed ID = 22503424 ] [ RRC reference ]

Than MT, Kudlow BA, Han M.
Functional analysis of neuronal microRNAs in Caenorhabditis elegans dauer formation by combinational genetics and Neuronal miRISC immunoprecipitation.
PLoS Genet 2013 9(6) e1003592
[ PubMed ID = 23818874 ] [ RRC reference ]

Ding XC, Grosshans H.
Repression of C. elegans microRNA targets at the initiation level of translation requires GW182 proteins.
EMBO J 2009 28(3) 213-22
[ PubMed ID = 19131968 ] [ RRC reference ]

Zhang L, Ding L, Cheung TH, Dong MQ, Chen J, Sewell AK, Liu X, Yates JR 3rd, Han M.
Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA targets by their interactions with GW182 proteins AIN-1 and AIN-2.
Mol Cell 2007 28(4) 598-613
[ PubMed ID = 18042455 ] [ RRC reference ]