Mutants (Isolated)

tm1552

Allele Nametm1552
Sequence NameF16F9.5
CGC Namemec-10
Worm BaseAllele Name tm1552
CGC Name mec-10
Sequence F16F9.5
Phenotypehomozygous viable. Dr. M. Driscoll: touch insensitive.
Mutation site30081/30082-30529/30530 (448 bp deletion)
ChromosomeX
Putative gene structurecomplement(join(27406..27579, 27668..27743, 27791..27919, 28137..28312, 28470..28565, 28627..28787, 29053..29153, 29197..29271, 29322..29404, 29454..29546, 29822..29898, 30056..30125, 30229..30525, 30576..30658, 30864..30985, 31232..31324, 31372..31640))
Map position0.01
Balancer
Map position of balancer
Sequence of primersExtRev:TGCTTGGGCAAGCTCCAAAC,IntRev:TGGGAGGGAGCTTCATCTTA,ExtFwd:GGTCCAGCTCTCACACTGGA,IntFwd:GTAGGGTCTGCAACTAGCTC
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Gonzales DL, Zhou J, Fan B, Robinson JT.
A microfluidic-induced C. elegans sleep state.
Nat Commun 2019 10(1) 5035 
[ PubMed ID = 31695031 ] [ RRC reference ]

Shi S, Luke CJ, Miedel MT, Silverman GA, Kleyman TR.
Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit.
J Biol Chem. 2016 291(27) 14012-22. 
[ PubMed ID = -1 ] [ RRC reference ]

Sood P, Murthy K, Kumar V, Nonet ML, Menon GI, Koushika SP.
Cargo crowding at actin-rich regions along axons causes local traffic jams.
Traffic 2018 19(3) 166-181 
[ PubMed ID = 29178177 ] [ RRC reference ]

Chatzigeorgiou M, Yoo S, Watson JD, Lee WH, Spencer WC, Kindt KS, Hwang SW, Miller DM 3rd, Treinin M, Driscoll M, Schafer WR.
Specific roles for DEG/ENaC and TRP channels in touch and thermosensation in C. elegans nociceptors.
Nat. Neurosci. 2010 13(7) 861-8 
[ PubMed ID = 20512132 ] [ RRC reference ]

Chatzigeorgiou M, Grundy L, Kindt KS, Lee WH, Driscoll M, Schafer WR.
Spatial asymmetry in the mechanosensory phenotypes of the C. elegans DEG/ENaC gene mec-10.
J. Neurophysiol. 2010 104(6) 3334-44 
[ PubMed ID = 20881202 ] [ RRC reference ]

Albeg A, Smith CJ, Chatzigeorgiou M, Feitelson DG, Hall DH, Schafer WR, Miller DM 3rd, Treinin M.
C. elegans multi-dendritic sensory neurons: morphology and function.
Mol. Cell. Neurosci. 2011 46(1) 308-17 
[ PubMed ID = 20971193 ] [ RRC reference ]

Husson SJ, Costa WS, Wabnig S, Stirman JN, Watson JD, Spencer WC, Akerboom J, Looger LL, Treinin M, Miller DM 3rd, Lu H, Gottschalk A.
Optogenetic analysis of a nociceptor neuron and network reveals ion channels acting downstream of primary sensors.
Curr. Biol. 2012 22(9) 743-52 
[ PubMed ID = 22483941 ] [ RRC reference ]

Cohen E, Yemini E, Schafer W, Feitelson DG, Treinin M.
Locomotion analysis identifies roles of mechanosensory neurons in governing locomotion dynamics of C. elegans.
J. Exp. Biol. 2012 215(Pt 20) 3639-48 
[ PubMed ID = 22811252 ] [ RRC reference ]

Sanders J, Nagy S, Fetterman G, Wright C, Treinin M, Biron D.
The Caenorhabditis elegans interneuron ALA is (also) a high-threshold mechanosensor.
BMC Neurosci 2013 14 156 
[ PubMed ID = 24341457 ] [ RRC reference ]

Li W, Kang L, Piggott BJ, Feng Z, Xu XZ.
The neural circuits and sensory channels mediating harsh touch sensation in Caenorhabditis elegans.
Nat Commun 2011 2 315 
[ PubMed ID = 21587232 ] [ RRC reference ]

Rabinowitch I, Chatzigeorgiou M, Schafer WR.
A gap junction circuit enhances processing of coincident mechanosensory inputs.
Curr. Biol. 2013 23(11) 963-7 
[ PubMed ID = 23707432 ] [ RRC reference ]

Mohammadi A, Byrne Rodgers J, Kotera I, Ryu WS.
Behavioral response of Caenorhabditis elegans to localized thermal stimuli.
BMC Neurosci 2013 14 66 
[ PubMed ID = 23822173 ] [ RRC reference ]

Chatzigeorgiou M, Schafer WR.
Lateral facilitation between primary mechanosensory neurons controls nose touch perception in C. elegans.
Neuron 2011 70(2) 299-309 
[ PubMed ID = 21521615 ] [ RRC reference ]

Arnadóttir J, O'Hagan R, Chen Y, Goodman MB, Chalfie M.
The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons.
J. Neurosci. 2011 31(35) 12695-704 
[ PubMed ID = 21880930 ] [ RRC reference ]

Russell J, Vidal-Gadea AG, Makay A, Lanam C, Pierce-Shimomura JT.
Humidity sensation requires both mechanosensory and thermosensory pathways in Caenorhabditis elegans.
Proc. Natl. Acad. Sci. U.S.A. 2014 111(22) 8269-74 
[ PubMed ID = 24843133 ] [ RRC reference ]

Zhang W, Bianchi L, Lee WH, Wang Y, Israel S, Driscoll M.
Intersubunit interactions between mutant DEG/ENaCs induce synthetic neurotoxicity.
Cell Death Differ. 2008 15(11) 1794-803 
[ PubMed ID = 18670436 ] [ RRC reference ]

Yemini E, Jucikas T, Grundy LJ, Brown AE, Schafer WR.
A database of Caenorhabditis elegans behavioral phenotypes.
Nat. Methods 2013 10(9) 877-9 
[ PubMed ID = 23852451 ] [ RRC reference ]