Mutants (Isolated)

tm1414

Allele Nametm1414
Sequence NameR06C7.1
CGC Namewago-1
Worm BaseAllele Name tm1414
CGC Name wago-1
Sequence R06C7.1
Phenotypehomozygous viable. Dr. C. Mello, Cell 127, 747-457 (2006).
Mutation site4662/4663-5644/5645 (982 bp deletion)
ChromosomeI
Putative gene structurejoin(3063..3227, 3274..3854, 3899..4631, 4680..4855, 4899..5074, 5117..5463, 5510..5749, 5790..6097, 6143..6254)
Map position1.85
Balancer
Map position of balancer
Sequence of primersExtFwd:CTCGAGATTTATGCCCCAAG,IntFwd:GCCCCAAGGGACAACTCTAT,ExtRev:CGGTCCATTGGAGCTTTGCA,IntRev:CGGATTCGTGACAGCGTTAT
Distributed lab
DepositorDr. S. Mitani
References Please submit your publication
Shirayama M, Seth M, Lee HC, Gu W, Ishidate T, Conte D Jr, Mello CC.
piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline.
Cell 2012 150(1) 65-77 
[ PubMed ID = 22738726 ] [ RRC reference ]

Gu W, Shirayama M, Conte D Jr, Vasale J, Batista PJ, Claycomb JM, Moresco JJ, Youngman EM, Keys J, Stoltz MJ, Chen CC, Chaves DA, Duan S, Kasschau KD, Fahlgren N, Yates JR 3rd, Mitani S, Carrington JC, Mello CC.
Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline.
Mol. Cell 2009 36(2) 231-44 
[ PubMed ID = 19800275 ] [ RRC reference ]

Zhou X, Xu F, Mao H, Ji J, Yin M, Feng X, Guang S.
Nuclear RNAi contributes to the silencing of off-target genes and repetitive sequences in Caenorhabditis elegans.
Genetics 2014 197(1) 121-32 
[ PubMed ID = 24532782 ] [ RRC reference ]

Hall SE, Chirn GW, Lau NC, Sengupta P.
RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans.
RNA 2013 19(3) 306-19 
[ PubMed ID = 23329696 ] [ RRC reference ]

Palominos MF, Verdugo L, Gabaldon C, Pollak B, Ortíz-Severín J, Varas MA, Chávez FP, Calixto A.
Transgenerational Diapause as an Avoidance Strategy against Bacterial Pathogens in Caenorhabditis elegans.
MBio 2017 8(5)  
[ PubMed ID = 29018118 ] [ RRC reference ]