Mutants (Isolated)

tm1184

Allele Name

tm1184

Allele Type

Normal

Sequence Name

ZK757.3

Gene Name

alg-4

Worm Base

Allele Name

tm1184

Gene Name

alg-4

Sequence

ZK757.3

Phenotype

homozygous viable. Dr. C. Mello, Cell 127, 747-457 (2006).

Mutation site

24969/24970-TTTTCTTC-25740/25741 (771 bp deletion + 8 bp insertion)

Chromosome

III

Putative gene structure

complement(join(Z30215.1:861..915, Z30215.1:201..373, 25676..25848, 24734..25627, 24473..24689, 23916..24423, 23604..23866, 23434..23558, 22889..23386, 22717..22841, 22579..22670))

Map position

1.14

Balancer

Map position of balancer

Sequence of primers

ExtFwd:CTTCCTTCGATCTTCCGTAC,IntFwd:GGCCAACGCGGGAAGTTCAT,ExtRev:GTTAGGCTAGCTGTAAGCAT,IntRev:GATCTCAGGATCTGGTTCAA

Distributed lab

Depositor

Dr. S. Mitani

References

Please submit your publication

Charlesworth AG, Seroussi U, Lehrbach NJ, Renaud MS, Sundby AE, Molnar RI, Lao RX, Willis AR, Woock JR, Aber MJ, Diao AJ, Reinke AW, Ruvkun G, Claycomb JM.
Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility.
Nucleic Acids Res 2021 49(15) 8836-8865
[ PubMed ID = 34329465 ] [ RRC reference ]

Zamanian M, Cook DE, Zdraljevic S, Brady SC, Lee D, Lee J, Andersen EC.
Discovery of genomic intervals that underlie nematode responses to benzimidazoles.
PLoS Negl Trop Dis 2018 12(3) e0006368
[ PubMed ID = 29601575 ] [ RRC reference ]

Conine CC, Batista PJ, Gu W, Claycomb JM, Chaves DA, Shirayama M, Mello CC.
Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans.
Proc Natl Acad Sci U S A 2010 107(8) 3588-93
[ PubMed ID = 20133686 ] [ RRC reference ]

Han T, Manoharan AP, Harkins TT, Bouffard P, Fitzpatrick C, Chu DS, Thierry-Mieg D, Thierry-Mieg J, Kim JK.
26G endo-siRNAs regulate spermatogenic and zygotic gene expression in Caenorhabditis elegans.
Proc Natl Acad Sci U S A 2009 106(44) 18674-9
[ PubMed ID = 19846761 ] [ RRC reference ]