Mutants (Isolated)

tm1094

Allele Name

tm1094

Allele Type

Normal

Sequence Name

C01G5.2

Gene Name

prg-2

Worm Base

Allele Name

tm1094

Gene Name

prg-2

Sequence

C01G5.2

Phenotype

homozygous viable. Dr. C. Mello, Cell 127, 747-457 (2006). Dr. E. Miska; Mol Cell 31, 79-90 (2008).

Mutation site

14468/14469-AAACAAGTGTTTAACAATTAAACAAGT-15533/15534 (1065 bp deletion + 27 bp insertion)

Chromosome

Putative gene structure

complement(join(13134..13289, 13335..13521, 13564..13801, 13849..14023, 14071..14435, 14479..14920, 14968..15210, 15380..15568, 15613..15786))

Map position

3.2

Balancer

Map position of balancer

Sequence of primers

IntRev:CGGGCCAACCATCAAAATGA,ExtRev:CTGGGAACTATCCAACCCGA,IntFwd:ATCTTACTTCGGACGAAGTG,ExtFwd:CAGCACCATCTCTGTAGAGA

Distributed lab

Depositor

Dr. S. Mitani

References

Please submit your publication

Shukla A, Perales R, Kennedy S.
piRNAs coordinate poly(UG) tailing to prevent aberrant and perpetual gene silencing.
Curr Biol 2021 31(20) 4473-4485.e3
[ PubMed ID = 34428467 ] [ RRC reference ]

Pereira AG, Gracida X, Kagias K, Zhang Y.
C. elegans aversive olfactory learning generates diverse intergenerational effects.
J Neurogenet 2020 34(3-4) 378-388
[ PubMed ID = 32940103 ] [ RRC reference ]

Zhou X, Xu F, Mao H, Ji J, Yin M, Feng X, Guang S.
Nuclear RNAi contributes to the silencing of off-target genes and repetitive sequences in Caenorhabditis elegans.
Genetics 2014 197(1) 121-32
[ PubMed ID = 24532782 ] [ RRC reference ]

Wang G, Reinke V.
A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis.
Curr Biol 2008 18(12) 861-7
[ PubMed ID = 18501605 ] [ RRC reference ]

Batista PJ, Ruby JG, Claycomb JM, Chiang R, Fahlgren N, Kasschau KD, Chaves DA, Gu W, Vasale JJ, Duan S, Conte D Jr, Luo S, Schroth GP, Carrington JC, Bartel DP, Mello CC.
PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans.
Mol Cell 2008 31(1) 67-78
[ PubMed ID = 18571452 ] [ RRC reference ]