Mutants (Isolated)

tm1086

Allele Nametm1086
Sequence NameT07E3.5
CGC Namebrc-2
Worm BaseAllele Name tm1086
CGC Name brc-2
Sequence T07E3.5
Phenotypelethal or sterile. Dr. S. Boulton: Mol Cell Biol 25, 3127-3139 (2005)
Mutation site3949/3950-4621/4622 (672 bp deletion)
ChromosomeIII
Putative gene structurecomplement(join(2953..2994, 3368..3485, 3551..3678, 3918..4014, 4061..4145, 4189..4311, 4363..4536, 4620..4759, 4804..4907, 5003..5153, 5229..5251))
Map position-0.91
BalancerhT2 [qIs48]
Map position of balancer
Sequence of primersExtRev:TTACGCTCCACCGGACAGTA,ExtFwd:TAACAGGGCGGAACACATAG,IntFwd:CCGGAGAAGTCCATAACTCG,IntRev:TGTGACATCGACCGATGGGT
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
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Exploring the link between MORF4L1 and risk of breast cancer.
Breast Cancer Res. 2011 13(2) R40 
[ PubMed ID = 21466675 ] [ RRC reference ]

Goodyer W, Kaitna S, Couteau F, Ward JD, Boulton SJ, Zetka M.
HTP-3 links DSB formation with homolog pairing and crossing over during C. elegans meiosis.
Dev. Cell 2008 14(2) 263-74 
[ PubMed ID = 18267094 ] [ RRC reference ]

Martin JS, Winkelmann N, Petalcorin MI, McIlwraith MJ, Boulton SJ.
RAD-51-dependent and -independent roles of a Caenorhabditis elegans BRCA2-related protein during DNA double-strand break repair.
Mol. Cell. Biol. 2005 25(8) 3127-39 
[ PubMed ID = 15798199 ] [ RRC reference ]

Petalcorin MI, Galkin VE, Yu X, Egelman EH, Boulton SJ.
Stabilization of RAD-51-DNA filaments via an interaction domain in Caenorhabditis elegans BRCA2.
Proc. Natl. Acad. Sci. U.S.A. 2007 104(20) 8299-304 
[ PubMed ID = 17483448 ] [ RRC reference ]

Shimizu T, Pastuhov SI, Hanafusa H, Matsumoto K, Hisamoto N.
The C. elegans BRCA2-ALP/Enigma Complex Regulates Axon Regeneration via a Rho GTPase-ROCK-MLC Phosphorylation Pathway.
Cell Rep 2018 24(7) 1880-1889 
[ PubMed ID = 30110643 ] [ RRC reference ]

Gao J, Kim HM, Elia AE, Elledge SJ, Colaiácovo MP.
NatB domain-containing CRA-1 antagonizes hydrolase ACER-1 linking acetyl-CoA metabolism to the initiation of recombination during C. elegans meiosis.
PLoS Genet. 2015 11(3) e1005029 
[ PubMed ID = 25768301 ] [ RRC reference ]

Saito TT, Mohideen F, Meyer K, Harper JW, Colaiácovo MP.
SLX-1 is required for maintaining genomic integrity and promoting meiotic noncrossovers in the Caenorhabditis elegans germline.
PLoS Genet. 2012 8(8) e1002888 
[ PubMed ID = 22927825 ] [ RRC reference ]

Pispa J, Palmén S, Holmberg CI, Jäntti J.
C. elegans dss-1 is functionally conserved and required for oogenesis and larval growth.
BMC Dev. Biol. 2008 8 51 
[ PubMed ID = 18471277 ] [ RRC reference ]

Youds JL, Barber LJ, Ward JD, Collis SJ, O'Neil NJ, Boulton SJ, Rose AM.
DOG-1 is the Caenorhabditis elegans BRIP1/FANCJ homologue and functions in interstrand cross-link repair.
Mol. Cell. Biol. 2008 28(5) 1470-9 
[ PubMed ID = 18086896 ] [ RRC reference ]

Ward JD, Muzzini DM, Petalcorin MI, Martinez-Perez E, Martin JS, Plevani P, Cassata G, Marini F, Boulton SJ.
Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair.
Mol. Cell 2010 37(2) 259-72 
[ PubMed ID = 20122407 ] [ RRC reference ]

Ward JD, Barber LJ, Petalcorin MI, Yanowitz J, Boulton SJ.
Replication blocking lesions present a unique substrate for homologous recombination.
EMBO J. 2007 26(14) 3384-96 
[ PubMed ID = 17611606 ] [ RRC reference ]

Wilson DM 3rd, Rieckher M, Williams AB, Schumacher B.
Systematic analysis of DNA crosslink repair pathways during development and aging in Caenorhabditis elegans.
Nucleic Acids Res. 2017 45(16) 9467-9480 
[ PubMed ID = 28934497 ] [ RRC reference ]