MBS (Other Mutants) - Detail

Strain No MBS679
Strain Name Bacillus subtilis (natto) NAFM79
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers bio-, γPGA+, amyE::pgsB-lacZ cm
Selection
Lastest Markers cm
Parent (Donor)
Parent (Recipient) Bacillus subtilis (natto) NAFM5
Method The procedure for the construction of the strain is described in the paper entitled "Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto)" (Biosci Biotechnol Biochem. (2009) 73(5),1149-55. The promoter region og pgsB gene was ligated with lacZ and introduced in amyE locus with cm.
Source
Culture Condition LB medium
Other Remarks
Reference Kimura K, Tran LS, Do TH, Itoh Y.
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci. Biotechnol. Biochem.  (2009)  73(5)   1149-55  
[PubMed ID = 19420703]  [RRC reference
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