MBS (Other Mutants) - Detail

Strain No MBS460
Strain Name Z5
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers trp+ (203, Z5), integrated the B. amyloliquefacience 203 portion around trp locus, from aroE and tyrA to aroB and aroF, in the chromosome
Selection
Lastest Markers
Parent (Donor) B. amyloliquefacience 203
Parent (Recipient) Bacillus subtilis 160
Method The procedure for the construction of the strain is described in the paper entitled "Gfenetic defects in DNA repair system and enhancement of intergenote transfoprmation eficiency in Bacillusu subtilis Marburg" (Mol. gen. Genet.(1978) 162, 229-235). Transformation of strain 15 (trpB160-argA15) with B. amyloliquefaciens 203 DNA. Intergenote trp+ (203, Z5), integrated the amyloliquefacience 203 portion around trp locus, from aroE, tyrA to aroB and aroF, in the chromosome.
Source
Culture Condition LB medium
Other Remarks
Reference Matsumoto K, Takahashi H, Saito H, Ikeda Y.
Genetic defects in DNA repair system and enhancement of intergenote transformation efficiency in Bacillus subtilis Marburg.
Mol Gen Genet  (1978)  162(3)   229-35  
[PubMed ID = 98700]  [RRC reference
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