The procedure for the construction of the strain is described in the paper entitled "Cloning, sequencing, and disruption of the Bacillus subtilispsd gene coding for phosphatidylserine decarboxylase" (J. Bacteriol.(1998)180, 100-106). The pss gene was replaced with spc by using MunI and HindIII sites.
Matsumoto K, Okada M, Horikoshi Y, Matsuzaki H, Kishi T, Itaya M, Shibuya I.
Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylase.
100-6 [PubMed ID = 9422599]