MBS (Other Mutants) - Detail

Strain No MBS425
Strain Name SDB02
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers trpB3 ΔpssA10::spc
Selection
Lastest Markers spc
Parent (Donor)
Parent (Recipient) Bacillus subtilis 160
Method The procedure for the construction of the strain is described in the paper entitled "Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylase" (J. Bacteriol.(1998)180, 100-106). The pss gene was replaced with spc by using MunI and HindIII sites.
Source
Culture Condition LB medium
Other Remarks
Reference Matsumoto K, Okada M, Horikoshi Y, Matsuzaki H, Kishi T, Itaya M, Shibuya I.
Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylase.
J Bacteriol  (1998)  180(1)   100-6  
[PubMed ID = 9422599]  [RRC reference
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