Strain No Strain Name Sex Plasmid Integrated Plasmid Autonomous Prophage Chromosomal Markers Parent Donor Parent Recipient Method Lastest Marker Selection Source Culture Condition Other Remarks Reference (RRC ID) MBS1 RIK539 trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnD1 delta-rrnE1 delta-rrnB2 delta-rrnI2 delta-rrnW2 delta-rrnJ1 :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS2 RIK540 trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnD1 delta-rrnE1 delta-rrnA1 delta-rrnI2 delta-rrnW2 delta-rrnJ1 :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS3 RIK541 trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnE1 delta-rrnB2 delta-rrnA1 delta-rrnI2 delta-rrnW2 delta-rrnJ1 :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS4 RIK542 trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnD1 delta-rrnE1 delta-rrnB2 delta-rrnA1 delta-rrnW2 delta-rrnJ1 :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS5 RIK543 trpC2 delta-rrnHG1 delta-rrnD1 delta-rrnE1 delta-rrnB2 delta-rrnA1 delta-rrnI2 delta-rrnW2 delta-rrnJ1 :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS6 RIK545 trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnD1 delta-rrnB2 delta-rrnA1 delta-rrnI2 delta-rrnW2 delta-rrnJ1 :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS7 RIK551 trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnD1 delta-rrnE1 delta-rrnB2 delta-rrnA1 delta-rrnI2 delta-rrnW3 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33566 MBS8 RIK900 trpC2 relA :: erm Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" Tryptophan auxotroph (trpC2) and erythromycin resistance (erm) Erythromycin resistance gene (erm) derived from pE194 L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS9 RIK908 trpC2 ywaC :: spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS10 RIK909 trpC2 yjbM :: cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS11 RIK913 trpC2 ywaC :: spc relA :: erm Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS12 RIK1000 trpC2 delta-yjbM Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS13 RIK1001 trpC2 delta-yjbM relA :: erm Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS14 RIK1003 trpC2 delta-yjbM ywaC :: spc relA :: erm Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33558 MBS15 RIK820 trpC2 rpsF::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS16 RIK822 trpC2 rpsT::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS17 RIK823 trpC2 rpsU::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS18 RIK824 trpC2 rplA::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS19 RIK825 trpC2 rplI::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS20 RIK826 trpC2 rplO::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS21 RIK827 trpC2 rplV::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS22 RIK828 trpC2 rplW::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS23 RIK830 trpC2 rpmB::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS24 RIK831 trpC2 rpmC::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS25 RIK832 trpC2 rpmF::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS26 RIK833 trpC2 rpmGA::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS27 RIK834 trpC2 rpmGB::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS28 RIK835 trpC2 rpmH::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS29 RIK836 trpC2 rpmI::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS30 RIK837 trpC2 rpmJ::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)\"" Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat) Chloramphenicol resistance gene (cat); pC194 derivative L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33575 MBS31 RIK1051 trpC2 aprE::Pspac-yjbM spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. (Microbiologyopen, [2012] 1(2): 115-134)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33574 MBS32 RIK1052 trpC2 aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. (Microbiologyopen, [2012] 1(2): 115-134)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33574 MBS33 RIK1371 trpC2 delta-yjbM ywaC::cat relA::erm aprE::Pspac-ywaC L176F spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. (Microbiologyopen, [2012] 1(2): 115-134)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33574 MBS34 RIK1375 trpC2 delta-yjbM ywaC::cat relA::erm aprE::Pspac-ywaC D87G spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. (Microbiologyopen, [2012] 1(2): 115-134)\"" L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on. 33574 MBS35 JEATdd trpC2 pdaC(yjeA)::tet Bacillus subtilis 168 homologous recombination tetracycline LB medium 33572 MBS36 HENCdd trpC2 yheN::cat Bacillus subtilis 168 homologous recombination chloramphenicol LB medium 33572 MBS37 XKHKdd trpC2 yxkH::km Bacillus subtilis 168 homologous recombination kanamycin LB medium 33572 MBS38 YLXYd trpC2 ylxY::(lacZ lacI bla erm) Bacillus subtilis 168 homologous recombination erythromycin LB medium 33572 MBS39 WE1 trpC2 epr::tet wpr::kan Bacillus subtilis EPRTc Bacillus subtilis WA homologous recombination tetracycline, kanamycin LB medium 33561 MBS40 WAC trpC2 ΔwprA Bacillus subtilis 168 homologous recombination none LB medium 33561 MBS41 WEC trpC2 Δepr ΔwprA Bacillus subtilis WAC homologous recombination none LB medium 33561 MBS42 168FKD trpC2 lytE::kan Bacillus subtilis 168 homologous recombination kanamycin LB medium 33561 MBS43 168FTD trpC2 lytE::tet Bacillus subtilis 168 homologous recombination tetracycline LB medium 33561 MBS44 168ESD trpC2 lytE::spc Bacillus subtilis 168 homologous recombination spectinomycin LB medium 33561 MBS45 168STD trpC2 cwlS::tet Bacillus subtilis 168 homologous recombination tetracycline LB medium 33561 MBS46 168YBSP trpC2 iseA::spc Bacillus subtilis 168 homologous recombination spectinomycin LB medium 33561 MBS47 168BKD trpC2 lytC::kan Bacillus subtilis 168 homologous recombination kanamycin LB medium 33561 MBS48 YCDDd trpC2 cwlK(ycdD)::erm Bacillus subtilis 168 homologous recombination erythromycin LB medium 33556 MBS49 168SDC trpC2 sigD::cat Bacillus subtilis 168 homologous recombination chloramphenicol LB medium 33556 MBS50 cdDSD trpC2 cwlK::erm sigD::cat Bacillus subtilis 168SDC Bacillus subtilis YCDDd homologous recombination erythromycin, chloramphenicol LB medium 33556 MBS51 YCDDp trpC2 cwlK::[Pspac-cwlK erm] Bacillus subtilis 168 homologous recombination erythromycin LB medium 33556 MBS52 YVRGHbDKm trpC2 yvrHGb::kan Bacillus subtilis 168 homologous recombination kanamycin LB medium 33553 MBS53 LYTRDTc trpC2 lytR::tet Bacillus subtilis 168 homologous recombination tetracycline LB medium 33553 MBS54 YDFHIDSp trpC2 ydfHI::spc Bacillus subtilis 168 homologous recombination spectinomycin LB medium 33550 MBS55 YDFJDPM4 trpC2 ydfJ::pM4YDFJ Bacillus subtilis 168 homologous recombination erythromycin LB medium 33550 MBS56 BANSdd trpC2 ybaN::spc Bacillus subtilis 168 homologous recombination spectinomycin LB medium 33548 MBS57 SSPEdg trpC2 sspE::pM4sspE-gfp Bacillus subtilis 168 homologous recombination erythromycin LB medium 33548 MBS58 BANSSPEdg trpC2 sspE::pM4sspE-gfp ybaN::spc Bacillus subtilis BANSdd homologous recombination erythromycin, spectinomycin LB medium 33548 MBS59 VCESD trpC2 cwlO(yvcE)::pM2-VCE lytF::spc Bacillus subtilis YVCEd Bacillus subtilis ESD homologous recombination erythromycin, spectinomycin LB medium 33552 MBS60 vcEBED trpC2 cwlO(yvcE)::pM2-VCE lytC::kan lytF::spc Bacillus subtilis BKD Bacillus subtilis VCESD homologous recombination kanamycin, spectinomycin LB medium 33552 MBS61 SIGDdd trpC2 sigD::cat Bacillus subtilis 168 homologous recombination chloramphenicol LB medium 33544 MBS62 MALLdd trpC2 malL::kan Bacillus subtilis 168 homologous recombination kanamycin LB medium 33536 MBS63 MLGLVAd trpC2 malL::kan glvA::pMV1 Bacillus subtilis MALLdd Bacillus subtilis GLVAd homologous recombination kanamycin, erythromycin LB medium 33536 MBS64 CITSTdd1Km trpC2 citST::kan Bacillus subtilis 168 homologous recombination kanamycin LB medium 33534 MBS65 CITSTdd2Tc trpC2 citST::tet Bacillus subtilis 168 homologous recombination tetracycline LB medium 33534 MBS66 SDCST trpC2 citS::pMSDCST (Pspac-citST) Bacillus subtilis 168 homologous recombination erythromycin LB medium 33534 MBS67 60015 trpC2 metC7 Bacillus subtilis 168 L medium 33567 MBS68 60866 Δigf trpC2 metC7 Not Bacillus subtilis 168 L medium 33585 MBS69 61111 metC7 trpC2 pgk Bacillus subtilis 168 L medium 33587 MBS70 61364 trpC2 metC7 pgi gpd Bacillus subtilis 168 L medium 33587 MBS71 61372 pgi trpC2 metC7 Bacillus subtilis 168 L medium 33587 MBS72 61402 glpD hisA trpC2 Bacillus subtilis 168 L medium 33587 MBS73 61411 pyrA3 accB fruB22 Bacillus subtilis 168 L medium 33587 MBS74 61447 trpC2 metC7 str pfk pgi Bacillus subtilis 168 L medium 33587 MBS75 61656 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33585 MBS76 61668 iol-6 trpC2 metC7 Bacillus subtilis 168 L medium 33586 MBS77 61774 Δigf bfdA1 hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33586 MBS78 QB885 thiC5 sacA321 purA16 Bacillus subtilis 168 L medium 33586 MBS79 QB944 =kit1 purA16 cysA14 trpC2 Bacillus subtilis 168 L medium 33586 MBS80 ISMRBE17 hsrM+ hsrR+ hsrB+ hsrE+ purB6 leuA8 metB5 Bacillus subtilis 168 L medium 33586 MBS81 NIG1131 met his spo0A34 Bacillus subtilis 168 L medium 33565 MBS82 CS3 lys-1 trpC2 crsA47 Bacillus subtilis 168 L medium 33592 MBS83 1A423 leuA8 thr-5 argA15 recE4 r(-) m(-) 168 Bacillus subtilis 168 L medium 33581 MBS84 YF001 trpC2 metB5 hisA1 leuA8 Bacillus subtilis 168 L medium 33585 MBS85 YF026 purA sacA321 ts199 Bacillus subtilis 168 L medium 33586 MBS86 YF029 gntP9 purA16 leuA8 metB5 hisA3 Bacillus subtilis 168 L medium 33586 MBS87 YF030 Δigf sacA321 Bacillus subtilis 168 L medium 33586 MBS88 YF062 lma-74(=fdp-74) bfdA1 glp trpC2 metB5 hisA1 leuA8 Bacillus subtilis 168 L medium 33586 MBS89 YF081 fdp-74 trpC2 hisA1 leuA8 metB5 Bacillus subtilis 168 L medium 33586 MBS90 YF086 Δigf leuA8 trpC2 purA16 Bacillus subtilis 168 L medium 33586 MBS91 YF100 Δigf hsrE+ Bacillus subtilis 168 L medium 33586 MBS92 YF111 iol-41 trpC2 metC7 Bacillus subtilis 168 L medium 29792 MBS93 YF125 iol-6 metB5 purB6 hsrM+ hsrR+ hsrB+ hsrE+ Bacillus subtilis 168 L medium 33586 MBS94 YF126 fdp-74 sacA321 purA16 Bacillus subtilis 168 L medium 33586 MBS95 YF127 gntK4 trpC2 metC7 Bacillus subtilis 168 L medium 33586 MBS96 YF130 fdp-74 iol-6 trpC2 metB5 hisA1 Bacillus subtilis 168 L medium 33586 MBS97 YF141 gntK4 trpC2 metB5 hisA1 Bacillus subtilis 168 L medium 33600 MBS98 YF149 fdp-74 gntK4 trpC2 metB5 hisA1 Bacillus subtilis 168 L medium 33586 MBS99 YF177 trpC2 recE4 gntR1 Bacillus subtilis 168 L medium 33583 MBS100 YF158 recE4 trpC2 gntK4 Bacillus subtilis 168 L medium 33581 MBS101 YF160 gntK10 trpC2 metC7 Bacillus subtilis 168 L medium 33581 MBS102 YF161 gntP23 trpC2 metC7 Bacillus subtilis 168 L medium 33581 MBS103 YF162 gntP26trpC2 metC7 Bacillus subtilis 168 L medium 33581 MBS104 YF168 lys-1 trpC2 Bacillus subtilis 168 L medium 33592 MBS105 YF169 trpC2 recE4 Bacillus subtilis 168 L medium 33582 MBS106 YF171 trpC2 metC7 gntP9 Bacillus subtilis 168 L medium 33580 MBS107 YF176 trpC2 metC7 gntR1 Bacillus subtilis 168 L medium 33583 MBS108 168Ti [ρ11] r11 thy ind Bacillus subtilis 168 L medium 33600 MBS109 61656 [ρ11phisA] r11phisA Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33600 MBS110 61656 [ρ11gnt+phisA-EcoRI] =E4 r11gnt+phisA-EcoRI Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33600 MBS111 61656 [ρ11gnt+phisA-BamHI] =B2 r11gnt+phisA-BamHI Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33600 MBS112 61656 [φ105] f105 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33581 MBS113 61656 [φ105gnt+] f105gnt+ Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33581 MBS114 61656 [φ105gnt+H2] f105gnt+H2 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 33581 MBS115 YF170 [φ105gntK4] f105gntK4 Δigf hisA1 metB5 trpC2 Bacillus subtilis 168 L medium 33580 MBS116 YF170 [φ105gntP9] f105gntP9 Δigf hisA1 metB5 trpC2 Bacillus subtilis 168 L medium 33580 MBS117 YF170 [φ105gntK10] f105gntK10 Δigf hisA1 metB5 trpC2 Bacillus subtilis 168 L medium 33583 MBS118 YF170 [φ105gntR1] f105gntR1 Δigf hisA1 metB5 trpC2 Bacillus subtilis 168 L medium 33583 MBS119 NIG1121 (pUB110) pUB110 met his Bacillus subtilis 168 kanamycin-r L medium 33581 MBS120 1E17 =168 (pC194) pC194 trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33581 MBS121 1A423 (pPL603B) pPL603B leuA8 thr-5 argA15 recE4 r(-) m(-) 168 Bacillus subtilis 168 kanamycin-r L medium 33578 MBS122 61656 (pCG1) pCG1 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33581 MBS123 61656 (pCG8) pCG8 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33581 MBS124 1A423 (pgnt21) pgnt21 leuA8 thr-5 argA15 recE4 r(-) m(-) 168 Bacillus subtilis 168 kanamycin-r L medium 33583 MBS125 1A423 (pgnt23) pgnt23 leuA8 thr-5 argA15 recE4 r(-) m(-) 168 Bacillus subtilis 168 kanamycin-r L medium 33578 MBS126 YF169 (pPL603BSauA) pPL603BSauA trpC2 recE4 Bacillus subtilis 168 kanamycin-r L medium 33582 MBS127 YF169 (pPL603BSauJA) pPL603BSSauJA trpC2 recE4 Bacillus subtilis 168 kanamycin-r L medium 33582 MBS128 61656 (pgnt41) pgnt41 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 29847 MBS129 61656 (pLS353) pLS353 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 29847 MBS130 DB204 lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 L medium 33579 MBS131 DB204 (pWP19) pWP19 lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 kanamycin-r L medium 33579 MBS132 DB204 (pgnt34) pgnt34 lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 kanamycin-r L medium 33579 MBS133 DB204 (pgnt37) pgnt37 lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 kanamycin-r L medium 33570 MBS134 DB204 (pgnt34dO) pgnt34dO lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 kanamycin-r L medium 33579 MBS135 DB204 (pgnt38) pgnt38 lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 kanamycin-r L medium 33568 MBS136 YF179 trpC2 metC7 gntOi Bacillus subtilis 168 L medium 33592 MBS137 DB204 (pWP19SA) pWP19SA lys-1 trpC2 phe-1 nprR2 nprE18 ΔaprA3 ispA1 Bacillus subtilis 168 kanamycin-r L medium 33568 MBS138 61656 (pgnt25) pgnt25 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33595 MBS139 61656 (pgnt24) pgnt24 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33595 MBS140 YF182 trpC2 metC7 gntC142A Bacillus subtilis 168 L medium 30133, 33593 MBS141 YF183 trpC2 metC7 gntC145G Bacillus subtilis 168 L medium 30133, 33593 MBS142 1A1 trpC2 Bacillus subtilis 168 L medium 33590 MBS143 GM122 trpC2 metC3 Bacillus subtilis 168 L medium 33595 MBS144 SA003 trpC2 metC3 ptsH1(S46A) Bacillus subtilis 168 L medium 33595 MBS145 1A147 alsA1 alsR1 ilvBD1 trpC2 Bacillus subtilis 168 L medium 33592 MBS146 WLN-29 aroG932 trpC2 gra-26::Tn917lac Bacillus subtilis 168 erythromycin-r L medium 33592 MBS147 1A250 alsR1 ilvBD1 trpC2 Bacillus subtilis 168 L medium 33592 MBS148 YF223 trpC2 metC7 gntR43L Bacillus subtilis 168 L medium 33592 MBS149 61656 [ρ11iol+] r11iol+ Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 L medium 29792 MBS150 1A272 [φ105hutH11] f105hutH11 hutH1 str sul Bacillus subtilis 168 L medium 30115 MBS151 YF230 trpC2 metC7 gntOi gntR48T (=supOi-2) Bacillus subtilis 168 L medium 33588 MBS152 YF231 trpC2 metC7 gntOi gntM4A (=supOi-1) Bacillus subtilis 168 L medium 33588 MBS153 YF234 trpC2 metC7 gntR48T Bacillus subtilis 168 L medium 33588 MBS154 1A250 (pCCPA110) pCCPA110 alsR1 ilvBD1 trpC2 Bacillus subtilis 168 kanamycin-r L medium 30125 MBS155 GM273 trpC2 sacR::lacZ DptsXHI::ermC Bacillus subtilis 168 erythromycin-r L medium 33562 MBS156 61656 (pGNT24M16T) pGNT24M16T Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33595 MBS157 61656 (pGNT24M39T) pGNT24M39T Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33595 MBS158 61656 (pGNT24M34T) pGNT24M34T Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33595 MBS159 61656 (pGNT24M11T) pGNT24M11T Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33595 MBS160 61656 (pGNT29) pGNT29 Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33594 MBS161 61656 (pGNT29P149T) pGNT29P149T Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33594 MBS162 61656 (pGNT29P154T) pGNT29P154T Δigf hisA1 leuA8 metB5 trpC2 Bacillus subtilis 168 kanamycin-r L medium 33594 MBS163 YF241 trpC2 metC7 gntOi gntR43L Bacillus subtilis 168 L medium 33588 MBS164 YF244 trpC2 metC7 iolR::Cm Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS165 YF246 trpC2 metC7 iolS::Cm Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS166 YF247 trpC2 metC7 gntOiM4A gntR43L Bacillus subtilis 168 L medium 33588 MBS167 YF248 trpC2 metC7 Piol::Cm Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS168 YF256 trpC2 metC7 iolE41(iol-41) iolR::cat Bacillus subtilis 168 chloramphenicol-r L medium 30180 MBS169 YF258 trpC2 metC7 iolB52 (iol-52) iolR::cat Bacillus subtilis 168 chloramphenicol-r L medium 30185 MBS170 YF259 trpC2 metC7 iolB58 (iol-58) iolR::cat Bacillus subtilis 168 chloramphenicol-r L medium 30185 MBS171 YF260 trpC2 metC7 iolC62 (iol-62) iolR::cat Bacillus subtilis 168 chloramphenicol-r L medium 30185 MBS172 YF282 trpC2 metC7 amyE::(Pgnt gntR'-'lacZ cat) Bacillus subtilis 168 chloramphenicol-r L medium 33595 MBS173 YF287 trpC2 sacR'-'lacZ amyE::[(Pgnt gntR'-'lacZ C→T -34) cat] Bacillus subtilis 168 chloramphenicol-r L medium 33595 MBS174 YF288 trpC2 sacR'-'lacZ amyE::[(Pgnt gntR'-'lacZ C→T +154) cat] Bacillus subtilis 168 chloramphenicol-r L medium 33595 MBS175 YF289 trpC2 sacR'-'lacZ amyE::[(Pgnt gntR'-'lacZ C→T -34T, +154) cat] Bacillus subtilis 168 chloramphenicol-r L medium 33595 MBS176 YF311 (= FDPAd) pMutin1 trpC2 fdpA::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 33597, 30151 MBS177 YF312 (= FDPAi) pMutin1 trpC2 PfdpA::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 33597, 30151 MBS178 YF315 (= SIGYi) pMutin1 trpC2 PsigY::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30179 MBS179 YF316 (= SIGYd) pMutin1 trpC2 sigY::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30179 MBS180 YF323 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS181 YF324 trpC2 metC7 amyE::[Piol(-45) iolA'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS182 YF325 trpC2 metC7 amyE::[Piol(-23) iolA'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS183 YF327 trpC2 metC7 amyE::[PiolRS(-94) iolR'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS184 YF328 trpC2 metC7 amyE::[PiolRS(-70) iolR'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS185 YF329 trpC2 metC7 amyE::[PiolRS(-43) iolR'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS186 YF330 trpC2 metC7 amyE::[PiolRS(-19) iolR'-'lacZ cat] Bacillus subtilis 168 chloramphenicol-r L medium 33596 MBS187 YF331 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ C→T -1 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS188 YF332 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ A→T +4 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS189 YF334 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ C→T +11 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS190 YF335 trpC2 metC7 amyE::[PiolRS(-94) iolR'-'lacZ C→T -23 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS191 YF336 trpC2 metC7 amyE::[PiolRS(-94) iolR'-'lacZ A→T -27 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS192 YF337 trpC2 metC7 amyE::[PiolRS(-94) iolR'-'lacZ C→T -23, A→T -27 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS193 YF338 trpC2 metC7 amyE::[PiolRS(-94) iolR'-'lacZ C→T -34 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS194 YF339 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ C→T -1, A→T +4 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS195 YF340 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ d31/168 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS196 YF341 trpC2 metC7 amyE::[Piol(-107) iolA'-'lacZ d22/168 cat] Bacillus subtilis 168 chloramphenicol-r L medium 33599 MBS197 FU339 pMutin2 trpC2 PasnO::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 33533 MBS198 FU340 trpC2 ΔasnB::neo Bacillus subtilis 168 neomycin-r L medium 33533 MBS199 FU341 trpC2 ΔasnH::spc Bacillus subtilis 168 spectinomycin-r L medium 33533 MBS200 FU342 trpC2 ΔasnO::cat Bacillus subtilis 168 chloramphenicol-r L medium 33533 MBS201 FU343 trpC2 ΔasnB::neo ΔasnH::spc Bacillus subtilis 168 neomycin-r, spectinomycin-r L medium 33533 MBS202 FU344 trpC2 ΔasnB::neo ΔasnO::cat Bacillus subtilis 168 neomycin-r, chloramphenicol-r L medium 33533 MBS203 FU345 trpC2 ΔasnH::spc ΔasnO::cat Bacillus subtilis 168 spectinomycin-r, chloramphnicol-r L medium 33533 MBS204 FU346 trpC2 ΔasnB::neo ΔasnH::spc ΔasnO::cat Bacillus subtilis 168 neomycin-r, spectinomycin-r, chloramphenicol-r L medium 33533 MBS205 FU347 trpC2 ytnA::pMutin2 ΔasnH::spc Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33533 MBS206 FU348 trpC2 ytnA::pMutin2 ΔasnH::spc ΔasnO::cat Bacillus subtilis 168 erythromycin-r, spectinomycin-r, chloramphenicol-r L medium 33533 MBS207 ASK201 trpC2 spoOH::erm Bacillus subtilis 168 erythromycin-r L medium 30179 MBS208 ASK202 trpC2 spoⅡAC::kan Bacillus subtilis 168 kanamycine-r L medium 30179 MBS209 ASK203 trpC2 spoⅡGAB::kan Bacillus subtilis 168 kanamycine-r L medium 30179 MBS210 ASK204 trpC2 spoⅢG::kan Bacillus subtilis 168 kanamycine-r L medium 30179 MBS211 ASK205 trpC2 spoⅣCB::erm Bacillus subtilis 168 erythromycin-r L medium 30179 MBS212 FU349 pMutin2 trpC2 ytrF::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 33535 MBS213 FU350 trpC2 ΔiolF Bacillus subtilis 168 L medium 33538 MBS214 FU351 pMutin2 trpC2 ΔiolF iolT::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 33538 MBS215 FU352 trpC2 ΔiolF iolR::cat Bacillus subtilis 168 chloramphenicol-r L medium 33538 MBS216 FU353 pMutin2 trpC2 iolT::pMutin2 iolR::cat Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33538 MBS217 FU354 pMutin2 trpC2 ΔiolF iolT::pMutin2 iolR::cat Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33538 MBS218 PS29 trpC2 gid::spc Bacillus subtilis 168 spectinomycin-r L medium 33539 MBS219 PS37 trpC2 gid::spc ΔcodY Bacillus subtilis 168 spectinomycin-r L medium 33539 MBS220 SF416 trpC2 ΔamyE::(ngrA-lacZ)416 neo Bacillus subtilis 168 neomycin-r L medium 33541 MBS221 SF416T trpC2 ΔamyE::(ngrA-lacZ)416 neo tnrA62::Tn917 Bacillus subtilis 168 neomycin-r, erythromycin-r L medium 33541 MBS222 FU355 trpC2 amyE::[PsigY sigY lacZ cat] Bacillus subtilis 168 chroramphenicol-r L medium 30179 MBS223 FU356 trpC2 amyE::[PsigY sigY' lacZ cat] Bacillus subtilis 168 chroramphenicol-r L medium 30179 MBS224 168 (pDG148-sigY) pDG148-sigY trpC2 Bacillus subtilis 168 L medium 33540 MBS225 FU357 trpC2 amyE::[PsigY sigY lacZ cat] spoOH::erm Bacillus subtilis 168 erythromycin-r, chroramphenicol-r L medium 30179 MBS226 FU358 trpC2 amyE::[PsigY sigY lacZ cat] spoⅡAC::kan Bacillus subtilis 168 kanamycin-r, chroramphenicol-r L medium 30179 MBS227 FU359 trpC2 amyE::[PsigY sigY lacZ cat] spoⅡGAB::kan Bacillus subtilis 168 kanamycin-r, chloramphenicol-r L medium 30179 MBS228 FU360 trpC2 amyE::[PsigY sigY lacZ cat] spoⅢG::kan Bacillus subtilis 168 kanamycin-r, chloramphenicol-r L medium 30179 MBS229 FU361 trpC2 amyE::[PsigY sigY lacZ cat] spoⅣCB::erm Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 30179 MBS230 FU382 pMutin2 trpC2 ilvB::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 33539 MBS231 FU383 pMutin2 trpC2 ilvD::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 33539 MBS232 FU385 pMutin2 trpC2 gid::spec ilvB::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS233 FU387 pMutin2 trpC2 gid::spec ilvD::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS234 FU389 pMutin2 trpC2 gid::spec ybgE::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS235 FU391 pMutin2 trpC2 gid::spec yufN::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS236 FU393 pMutin2 trpC2 gid::spec yufO::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS237 FU395 pMutin2 trpC2 gid::spec yurP::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS238 FU397 pMutin2 trpC2 gid::spec yurN::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS239 FU399 pMutin2 trpC2 gid::spec ykfA::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS240 FU401 pMutin2 trpC2 gid::spec yhdG::pMutin2 ΔcodY Bacillus subtilis 168 erythromycin-r, spectinomycin-r L medium 33539 MBS241 FU402 trpC2 ccpA::neo Bacillus subtilis 168 neomycin-r L medium 33560 MBS242 FU408 trpC2 gid::spec ΔcodY amyE::(cat yufN-lacZ) Bacillus subtilis 168 spectinomycin-r, chloramphenicol-r L medium 33539 MBS243 QB5223 trpC2 pstH1 Bacillus subtilis 168 L medium 33537 MBS244 QB7096 trpC2 crh::aphA3 Bacillus subtilis 168 kanamycin-r L medium 33537 MBS245 QB7102 trpC2 pstH1 crh::aphA3 Bacillus subtilis 168 kanamycin-r L medium 33537 MBS246 FU409 trpC2 amyE::[cat ΔPsigY-sigY(-4/+568)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 30179 MBS247 FU410 trpC2 amyE::[cat 'PsigY-sigY(-12/+568)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 30179 MBS248 FU411 trpC2 amyE::[cat PsigY-sigY(-39/+568)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 30179 MBS249 FU412 trpC2 amyE::[cat PsigY-sigY(-55/+568)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 30179 MBS250 FU428 pMutin2 trpC2 metC7 iolA::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS251 FU429 pMutin2 trpC2 metC7 iolB::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS252 FU430 pMutin2 trpC2 metC7 iolC::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS253 FU431 pMutin2 trpC2 metC7 iolD::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS254 FU432 pMutin2 trpC2 metC7 iolE::pMutin2 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS255 FU433 pMutin1 trpC2 metC7 iolF::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS256 FU434 pMutin1 trpC2 metC7 iolG::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS257 FU435 pMutin1 trpC2 metC7 iolH::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS258 FU436 pMutin1 trpC2 metC7 iolI:pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS259 FU437 pMutin1 trpC2 metC7 iolJ::pMutin1 Bacillus subtilis 168 erythromycin-r L medium 30180 MBS260 FU459 trpC2 tnrA::cat Bacillus subtilis 168 chloramphenicol-r L medium 33547 MBS261 1A765 (=BR16) trpC2 lys Bacillus subtilis 168 L medium 33560 MBS262 1A766 (=BR17) trpC2 lys relA Bacillus subtilis 168 L medium 33560 MBS263 FU652 ccpA::cat trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33549 MBS264 FU657 pMutin2 ysnD::pMutin2 tnrA::cat Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33547 MBS265 FU659 tnrA::Tn917(erm) typC2 Bacillus subtilis 168 erythromycin-r L medium 33547 MBS266 FU676 trpC2 amyE::[cat P(ilv-leu)-ilvB(-248/+26)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33547 MBS267 FU696 pMutin2 trpC2 ccpA::neo ilvB::pMutin2 Bacillus subtilis 168 erythromycin-r, neomycin-r L medium 33549 MBS268 FU698 pMutin2 rpC2 tnrA::cat ilvB::pMutin2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33547 MBS269 FU706 pMutin2 trpC2 gid::spec ΔcodY ccpA::neo ilvB::pMutin2 Bacillus subtilis 168 erythromycin-r, neomycin-r, spectinomycin-r L medium 33549 MBS270 FU707 trpC2 ΔglnQ::cat Bacillus subtilis 168 chloramphenicol-r L medium 33551 MBS271 FU708 pMutin2 ΔglnQ::cat ybgH::pMutin2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33551 MBS272 FU709 trpC2 amyE::[cat P(ilv-leu)(-187/+26)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS273 FU710 trpC2 amyE::[cat P(ilv-leu)(-667/+26)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS274 FU713 trpC2 amyE::[cat P(ilv-leu)(-248/+26)TnrA box(A-207C C-196T)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33547 MBS275 FU714 trpC2 amyE::[cat P(ilv-leu)(-165/+26)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS276 FU715 trpC2 amyE::[cat P(ilv-leu)(-150/+26)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS277 FU716 trpC2 amyE::[cat P(ilv-leu)(-100/+26)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS278 FU717 trpC2 amyE::[cat P(ilv-leu)(-55/+26)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS279 FU722 trpC2 amyE::[cat P(ilv-leu)(-248/+26)cre(G-89T C-84T)-lacZ] Bacillus subtilis 168 chloramphnicol-r L medium 33549 MBS281 PLR1 Bacillus subtilis 168 L medium 30181 MBS282 PLR2 Bacillus subtilis 168 L medium 30181 MBS283 1A221 lin-2 Bacillus subtilis 168 L medium 30181 MBS284 LCFAd pMutin lcfA::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33554 MBS285 ACDAd pMutin acdA::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33554 MBS286 ETFAd pMutin etfA::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33554 MBS287 ETFBd pMutin etfB::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33554 MBS288 CYDAd pMutin cydA::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33554 MBS289 CYDBd pMutin cydB::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33598 MBS290 CYDCd pMutin cydC::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33598 MBS291 CYDDd pMutin cydD;;pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33598 MBS292 LMRAd pMutin lmrA::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33555 MBS293 LMRBd pMutin lmrB::pMutin trpC2 Bacillus subtilis 168 erythromycin-r L medium 33555 MBS294 FU801 pMutin trpC2 gltA glsA (=ybgJ)::pMutin Bacillus subtilis 168 erythromycin-r L medium 33551 MBS295 FU807 trpC2 lys relA gid::spec ΔcodY Bacillus subtilis 168 spectinomycin-r L medium 33564 MBS296 FU808 trpC2 lys gid::spec ΔcodY Bacillus subtilis 168 spectinomycin-r L medium 33564 MBS297 61884 trpC2 aspB66 Bacillus subtilis 168 L medium 33562 MBS298 NIG2001 trpC2 pheA1 rpoC::pETΔrpoC (=rpoC-His6 neo) Bacillus subtilis 168 neomycin-r L medium 33560 MBS299 FU875 trpC2 yxaF::cat Bacillus subtilis 168 chloramphenicol-r L medium 33555 MBS300 FU876 trpC2 lmrA::tet Bacillus subtilis 168 tetracycline-r L medium 33555 MBS301 FU877 pMutin trpC2 lmrA::pMutin yxaF::cat Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33555 MBS302 FU878 pMutin trpC2 lmrB::pMutin yxaF::cat Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33555 MBS303 FU879 pMutin trpC2 yxaG::pMutin yxaF::cat Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33555 MBS304 FU880 pMutin trpC2 yxaG::pMutin lmrA::tet Bacillus subtilis 168 erythromycin-r, tetracycline-r L medium 33555 MBS305 FU881 pMutin trpC2 yxaG::pMutin yxaF::cat lmrA::tet Bacillus subtilis 168 erythromycin-r, chloramphenicol-r, tetracycline-r L medium 33555 MBS306 FU884 trpC2 ysiA::tet Bacillus subtilis 168 tetracycline-r L medium 33569 MBS307 FU895 trpC2 lys amyE::[cat P(ilv-leu)(-55/+26)(C-1G)- lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33560 MBS308 FU899 lmrA(Q52 stop to S) yxaF::cat trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33555 MBS309 FU904 trpC2 lys amyE::[cat P(ilv-leu)(-55/+26)(C+1G)(A+2G)- lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33560 MBS310 FU905 trpC2 lys amyE::[cat P(ilv-leu)(-55/+26)(A+2G)- lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33560 MBS311 FU906 trpC2 lys amyE::[cat PptsG(-55/+26) - lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33564 MBS312 FU934 trpC2 lys amyE::[cat PptsG (-55/+26)(G+2A) - lacZ Bacillus subtilis 168 chloramphenicol-r L medium 33564 MBS313 FU937 trpC2 lys amyE::[cat PalsS (-55/+26) - lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33564 MBS314 FU960 trpC2 amyE::[PlcfBΔcre(-120/+484) cat] Bacillus subtilis 168 chloramphenicol-r L medium 33569 MBS315 FU961 trpC2 amyE::[PlcfB cre(-120/+516) cat] Bacillus subtilis 168 chloramphenicol-r L medium 33569 MBS316 FU967 pMutin lmrA::tet yxaF::cat yxaH::pMutin Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33555 MBS317 FU977 trpC2 lys amyE::[cat PpycA(-55/+26) - lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33564 MBS318 FU987 168 trpC2 amyE::[cat PlcfAΔcre-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33569 MBS319 FU988 168 trpC2 amyE::[cat PlcfAΔcre-lacZ] fadR::tet Bacillus subtilis 168 chloramphenicol-r, tetracycline-r L medium 33569 MBS320 FU989 168 trpC2 amyE::[cat PlcfAcre-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33569 MBS321 FU991 168 trpC2 amyE::[cat PfadNDcre-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33569 MBS322 FU993 168 trpC2 amyE::[cat PfadN cre-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33569 MBS323 FU1019 trpC2 lys amyE::[cat Ppdh(-47/+167) - lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33564 MBS324 FU1033 trpC2 yetL::cat Bacillus subtilis 168 chloramphenicol-r L medium 33563 MBS325 FU1034 trpC2 yetL::tet Bacillus subtilis 168 tetracycline-r L medium 33563 MBS326 FU1035 trpC2 amyE::[cat PyetL(-118 to +28)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33563 MBS327 FU1036 trpC2 amyE::[cat PyetL(-334 to +228)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33563 MBS328 FU1037 trpC2 amyE::[cat PyetM(-313 to +249)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33563 MBS329 FU1042 trpC2 lys amyE::[cat PptsG (-55/+26)(G+1A)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33564 MBS330 FU1044 trpC2 lys amyE::[cat PpdhA (-47/+167)(G+1A)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33564 MBS331 FU1045 trpC2 lys amyE::[cat PptsG (-55/+26)(G+1A, G+2A)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33564 MBS332 FU1048 amyE::[cat PlcfA(-190?/+711?)(with cre)-lacZ] fadR::tet ccpA::neo trpC2 Bacillus subtilis 168 chloramphencicol-r, tetracycline-r, neomycin-r L medium 33569 MBS333 FU1060 trpC2 lys amyE::[cat PpycA(-55/+26)(A+1G)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33564 MBS334 FU1061 trpC2 lys amyE::[cat PalsS (-55/+26)(A+1G)-lacZ] Bacillus subtilis 168 chloramphenicl-r L medium 33564 MBS335 FU1074 amyE::[ cat PlcfB(-120/+516) (with cre)-lacZ] ccpA::neo trpC2 Bacillus subtilis 168 chloramphenicl-r, neomycin-r L medium 33569 MBS336 FU1078 lmrA2 [=lmrA(Q52P stop to S] ΔqdoR::tet Bacillus subtilis 168 tetracycline-r L medium 30187 MBS337 FU1079 lmrA2 ΔqdoR::tet amyE::[cat PyxaF-yxaF-PyxaG(-67/+692)-lacZ] Bacillus subtilis 168 tetracycline-r, chloramphenicol-r L medium 30187 MBS338 FU1080 lmrA2 ΔqdoR::tet amyE::[cat PyxaF-yxaF(W131A)-PyxaG(-67/+692)-lacZ] Bacillus subtilis 168 tetracycline-r, chloramphenicol-r L medium 30187 MBS339 FU1084 trpC2 fadR::tet ccpA::neo Bacillus subtilis 168 tetracycline-r, neomycin-r L medium 33569 MBS340 FU1087 trpC2 amyE::[cat PkinA(-55/+10)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33576 MBS341 FU1088 trpC2 amyE::[cat PkinA(-55/+10)(A+1G)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33576 MBS342 FU1095 trpC2 kinA::erm Bacillus subtilis 168 erythromycin-r L medium 33576 MBS343 FU1096 trpC2 kinB::kan Bacillus subtilis 168 kanamycin-r L medium 33576 MBS344 FU1098 trpC2 kinA::erm kinB::kan Bacillus subtilis 168 erythromycin-r, kanamycin-r L medium 33576 MBS345 FU1102 trpC2 kinA(A+1G) Bacillus subtilis 168 L medium 33576 MBS346 FU1103 trpC2 kinA(A+1G) kinB::kan Bacillus subtilis 168 kanamycin-r L medium 33576 MBS347 FU1106 trpC2 ΔabrB::erm Bacillus subtilis 168 eythromycin-r L medium 33576 MBS348 FU1107 trpC2 Δspo0H::erm amyE::[cat PkinA (-55/+10) -lacZ] Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33576 MBS349 FU1108 trpC2 Δspo0H::erm amyE::[cat PkinA (-55/+10)(A+1G)-lacZ] Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33576 MBS350 FU1113 trpC2 kinB (A+1G) Bacillus subtilis 168 L medium 33576 MBS351 FU1115 trpC2 amyE::[cat PkinB (-55/+10)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33576 MBS352 FU1116 trpC2 amyE::[cat PkinB (-55/+10)(A+1G)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33576 MBS353 FU1117 trpC2 Δspo0H::erm amyE::[cat PkinB (-55/+10)-lacZ] Bacillus subtilis 168 chloramphenicol-r, erythromycin-r L medium 33576 MBS354 FU1121 Δspo0A::spc trpC2 Bacillus subtilis 168 spectinomycin-r L medium 33576 MBS355 PS37t trpC2 gid::spc ΔcodY Bacillus subtilis 168 spectinomycin-r L medium 33576 MBS356 FU1130 ΔycnK::cat trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33573 MBS357 FU1131 ΔcsoR::cat trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33573 MBS358 FU1132 ΔcsoR::tet trpC2 Bacillus subtilis 168 tetracycline-r L medium 33573 MBS359 FU1133 ΔcopZA::cat trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33573 MBS360 FU1134 Δ(csoR copZA)::cat trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33573 MBS361 FU1135 pMutin2 ΔcsoR::cat ycnK::pMutin2 trpC2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33573 MBS362 FU1136 pMutin2 ΔcopZA::cat ycnK::pMutin2 trpC2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33573 MBS363 FU1137 pMutin2 Δ(csoR copZA)::cat ycnK::pMutin2 trpC2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33573 MBS364 FU1138 pMutin2 ΔcsoR::cat ycnJ::pMutin2 trpC2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33573 MBS365 FU1139 pMutin2 ΔcopZA::cat ycnJ::pMutin2 trpC2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33573 MBS366 FU1140 pMutin2 Δ(csoR copZA)::cat ycnJ::pMutin2 trpC2 Bacillus subtilis 168 erythromycin-r, chloramphenicol-r L medium 33573 MBS367 FU1144 ΔycnK::erm trpC2 Bacillus subtilis 168 erythromycin-r L medium 33573 MBS368 FU1145 ΔcopZA::tet trpC2 Bacillus subtilis 168 tetracycline-r L medium 33573 MBS369 FU1146 Δ(csoR copZA)::tet trpC2 Bacillus subtilis 168 tetracycline-r L medium 33573 MBS370 FU1147 amyE::[cat PycnK(-190 to 109)-lacZ] trpC2 Bacillus subtilis 168 chloramphenicol-r L medium 33573 MBS371 FU1153 trpC2 amyE::[cat PkinA (-55/+10)(A+1C)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33576 MBS372 FU1154 trpC2 amyE::[cat PkinB (-55/+10)(A+1C)-lacZ] Bacillus subtilis 168 chloramphenicol-r L medium 33576 MBS373 FU1155 trpC2 kinA (A+1G) kinB (A+1G) Bacillus subtilis 168 L medium 33576 MBS374 FU1156 trpC2 kinA (A+1C) Bacillus subtilis 168 L medium 33576 MBS375 RIK218 trpC2 ΔrrnW1 : : cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS376 RIK546 trpC2 ΔrrnW3 ΔrrnJ1 : : cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS377 RIK1755 trpC2 ΔrrnHG1 ΔrrnW2 : : cat ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS378 RIK1753 trpC2 ΔrrnHG1 ΔrrnW2 ΔrrnJ1 : : spc ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS379 RIK1466 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnW2 ΔrrnJ1 : : kan ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS380 RIK1463 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnB2 ΔrrnW2 ΔrrnJ1 : : kan ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS381 RIK1437 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1 : : spc ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS382 RIK1754 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1 : : cat ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" 33577 MBS383 RIK2222 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS384 RIK2223 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS385 RIK2224 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS386 RIK2225 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS387 RIK2226 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS388 RIK2227 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnA1 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS389 RIK2228 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnE1 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS390 RIK2229 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnE1 ΔrrnB2 ΔrrnA1 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS391 RIK2230 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnA1 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS392 RIK2231 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnB2 ΔrrnA1 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS393 RIK2232 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnE1 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS394 RIK2233 trpC2 ΔrrnHG1 ΔrrnB2 ΔrrnE1 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS395 RIK2234 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnB2 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS396 RIK2235 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS397 RIK2236 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnB2 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔrrnJ1 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS398 RIK2237 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnB2 ΔrrnE1 ΔrrnI2 ΔrrnW2 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS399 RIK2238 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnA1 ΔrrnE1 ΔrrnI2 ΔrrnW2 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS400 RIK2239 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnB2 ΔrrnA1 ΔrrnE1 ΔrrnI2 ΔrrnW2 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS401 RIK2240 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnB2 ΔrrnA1 ΔrrnE1 ΔrrnW2 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS402 RIK2241 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnB2 ΔrrnA1 ΔrrnE1 ΔrrnI2 ΔrrnW2 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS403 RIK2242 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnB2 ΔrrnA1 ΔrrnI2 ΔrrnW2 ΔICEBs1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled Multiple rRNA operons are essential for efficient cellgrowth and sporulation as well as outgrowth inBacillus subtilis\"(Microbiology (2013), 159, 2225–2236)" L medium 33577 MBS404 RIK1004 trpC2 ywaC::cat Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS405 RIK1054 trpC2 relA::erm aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS406 RIK1055 trpC2 ΔyjbM aprE::Pspac-yjbM spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS407 RIK1056 trpC2 ΔyjbM aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS408 RIK1057 trpC2 ΔyjbM ywaC:: cat aprE::Pspac-yjbM spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS409 RIK1058 trpC2 ΔyjbM ywaC:: cat aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS410 RIK1059 trpC2 ΔyjbM ywaC:: cat relA::erm aprE::Pspac-yjbM spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS411 RIK1066 trpC2 ΔyjbM ywaC:: cat relA::erm aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS412 RIK1068 trpC2 ΔyjbM relA::erm aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS413 RIK1069 trpC2 yvyD::PrrnO-kan Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS414 RIK1070 trpC2 ΔyjbM ywaC:: cat relA::erm aprE::Pspac-ywaC spc yvyD::PrrnO-kan Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS415 RIK1096 trpC2 ywaC:: cat aprE::Pspac-yjbM spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS416 RIK1098 trpC2 relA::erm ywaC::cat aprE::Pspac-ywaC spc Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS417 RIK1392 pURI7yvyD trpC2 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS418 RIK1420 trpC2 rrnO2+::kmpt1 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome\"(Microbiologyopen (2012), 1, 115-134)" LB medium 33574 MBS419 RIK1285 trpC2 lys1 nprR2 nprE18 aprEΔ3 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Spontaneous transformation and its use for genetic mapping in Bacillus subtilis.\"(Biosci. Biotechnol. Biochem. 68: 1672-1680 (2004))" L medium 33546 MBS420 OK2 bio The B. subtilis natto strain OK2 was isolated from a commercial fermented soybean natto, “Okame natto,” produced by Takano Foods Co. in Ibaraki, Japan. LB medium 36924 MBS421 RIK7101 bio amyE::comG-lacZ OK2 "The detailed procedures for the construction of the strain are described in the paper entitled \"Natural genetic competence in Bacillus subtilis natto OK2.\" [J Bacteriol (2000) 182, 2411-2415.]" LB medium 36924 MBS422 RIK7102 bio amyE::comG-lacZ mecA::spc OK2 "The detailed procedures for the construction of the strain are described in the paper entitled \"Natural genetic competence in Bacillus subtilis natto OK2.\" [J Bacteriol (2000) 182, 2411-2415.]" LB medium 36924 MBS423 RIK1027 trpC2 amyE::[comG-lacZ (Cmr)] pULI7KS27 Bacillus subtilis 168 "The detailed procedures for the construction of the strain are described in the paper entitled \"Natural genetic competence in Bacillus subtilis natto OK2.\" [J Bacteriol (2000) 182, 2411-2415.]" LB medium 36924 MBS424 SDB01 trpB3 psd1::neo Bacillus subtilis 160 "The procedure for the construction of the strain is described in the paper entitled \"Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylase\" (J. Bacteriol.(1998)180, 100-106). The psd gene was interrupted at the unique PstI site with neo. " neo LB medium 48565 MBS425 SDB02 trpB3 ΔpssA10::spc Bacillus subtilis 160 "The procedure for the construction of the strain is described in the paper entitled \"Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylase\" (J. Bacteriol.(1998)180, 100-106). The pss gene was replaced with spc by using MunI and HindIII sites." spc LB medium 48565 MBS426 160 trpB3 Hiuga Saito LB medium 48565, 48564 MBS427 SDB201 trpC2 ywjE1::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Cardiolipin domains in Bacillus subtilis Marburg membranes \" (J. Bacteriol. (2004)186, 1475-1483). The ywJE gene was interrupted at the unique HindIII site with spc. " spc LB medium 48566 MBS428 SDB202 trpC2 ywiE2::neo Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Cardiolipin domains in Bacillus subtilis Marburg membranes \" (J. Bacteriol. (2004)186, 1475-1483). The ywiE gene was interrupted at an introduced XbaI site with neo. " neo LB medium 48566 MBS429 SDB203 trpC2 clsA::pMutin4 ywiE2::neo SDB202 DNA BFS219 (trpC2 clsA::pMutin4) "The procedure for the construction of the strain is described in the paper entitled \"Cardiolipin domains in Bacillus subtilis Marburg membranes \" (J. Bacteriol. (2004)186, 1475-1483). " neo LB medium 48566 MBS430 SDB206 trpC2 clsA::pMutin4 ywiE2::neo ywjE1::spc SDB201 DNA SDB203 (trpC2 clsA::pMutin4 ywiE2::neo) "The procedure for the construction of the strain is described in the paper entitled \"Cardiolipin domains in Bacillus subtilis Marburg membranes \" (J. Bacteriol. (2004)186, 1475-1483). " spc LB medium 48566 MBS431 SDB210 trpC2 clsA::pMutin4 ywiE2::neo ywjE1spc ΔpssA::cat SDB211 DNA SDB206 (trpC2 clsA::pMutin4 ywiE2::neo ywjE1::spc) "The procedure for the construction of the strain is described in the paper entitled \"Cardiolipin enrichment in spore membranes and its involvement in germination of Bacillus subtilis Marburg membranes\" (Genes Genet. Syst. (2006) 81, 69-76). " cat DSM 48568 MBS432 SDB211 trpC2 ΔpssA::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Cardiolipin enrichment in spore membranes and its involvement in germination of Bacillus subtilis Marburg membranes\" (Genes Genet. Syst. (2006) 81, 69-76). " cat DSM 48568 MBS433 SDB011 trpC2 Pspac-cdsA Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pMUTIN2 was used to construct the Pspac-inducible allele. " ery DSM 48567 MBS434 SDB012 trpC2 Pspac-yhdO (plsC) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pMUTIN2 was used to construct thePspac-inducible allele. " ery DSM 48567 MBS435 SDB014 trpC2 mprF::tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). The mprF gene was interrupted at an introduced ClaI site with tet. " tet DSM 48567 MBS436 SDB110 trpC2 Pspac-pgsA Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes \" (J. Bacteriol. (2005) 187, 2163-2174). pMUTIN2 was used to construct the Pspac-inducible allele. " ery DSM 48567 MBS437 SDB1001 trpC2 amyE::PcitM-gfp cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pDHCMGFP was used to construct the control for Pspac-inducible allele. " cat DSM 48567 MBS438 SDB1006 trpC2 amyE::Pxyl-gfp-pgsA spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS439 SDB1010 trpC2 amyE::PcitM-pssA-gfp cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pDHCMGFP was used to construct the Pspac-inducible allele. " cat DSM 48567 MBS440 SDB1012 trpC2 amyE::PcitM-gpsA-gfp cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pDHCMGFP was used to construct the Pspac-inducible allele. " cat DSM 48567 MBS441 SDB1014 trpC2 amyE::Pxyl-gfp-psd spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS442 SDB1017 trpC2 amyE::Pxyl-gfp-mprF spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS443 SDB1018 trpC2 amyE::Pxyl-gfp-cdsA spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS444 SDB1019 trpC2 amyE::Pxyl-gfp-yhdO (plsC) spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS445 SDB1020 trpC2 amyE::PcitM-ugtP-gfp cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes \" (J. Bacteriol. (2005) 187, 2163-2174). pDHCMGFP was used to construct the Pspac-inducible allele. " cat DSM 48567 MBS446 SDB1021 trpC2 amyE::Pxyl-gfp-ugtP spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS447 SDB1022 trpC2 amyE::Pxyl-gfp-dgkA spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS448 SDB1101 trpC2 amyE::Pxyl-gfp-clsA spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes\" (J. Bacteriol. (2005) 187, 2163-2174). pSG1729 was used to construct the Pxyl-inducible gfp fusion allele. " spc DSM 48567 MBS449 MBS10 trpC2 ΔltaS::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis\" (Microbiology. (2013) 159, 23-35)." spc LB medium 48570 MBS450 MBS11 trpC2 ΔyfnI::tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis\" (Microbiology. (2013) 159, 23-35)." tet LB medium 48570 MBS451 MBS12 trpC2 ΔltaS::spc ΔyfnI::tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS452 MHB001 trpC2 Pspac-pgsA erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with membranes of reduced phosphatidylglycerol content\" (Genes Genet. Syst. (2009) 84, 191-198. pMUTINCC which is the pMUTIN3 having two Oid was used to construct the Pspac-pgsA inducible allele." ery DSM 48569 MBS453 MHB300 trpC2 ΔltaS::spc ΔyfnI::tet Pspac-pgsA erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." ery LB medium 48570 MBS454 SLD03 trpC2 ΔyqgS Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS455 SLD04 trpC2 ΔyvgJ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS456 SLD05 trpC2 ΔyfnI::tet ΔyqgS Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS457 SLD06 trpC2 ΔltaS::spc ΔyfnI::tet ΔyqgS Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS458 SLD07 trpC2 ΔyfnI::tet ΔyqgS ΔyvgJ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS459 SLD08 trpC2 ΔltaS::spc ΔyfnI::tet ΔyqgS ΔyvgJ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis \" (Microbiology. (2013) 159, 23-35)." LB medium 48570 MBS460 Z5 trp+ (203, Z5), integrated the B. amyloliquefacience 203 portion around trp locus, from aroE and tyrA to aroB and aroF, in the chromosome B. amyloliquefacience 203 Bacillus subtilis 160 "The procedure for the construction of the strain is described in the paper entitled \"Gfenetic defects in DNA repair system and enhancement of intergenote transfoprmation eficiency in Bacillusu subtilis Marburg\" (Mol. gen. Genet.(1978) 162, 229-235). Transformation of strain 15 (trpB160-argA15) with B. amyloliquefaciens 203 DNA. Intergenote trp+ (203, Z5), integrated the amyloliquefacience 203 portion around trp locus, from aroE, tyrA to aroB and aroF, in the chromosome." LB medium 48564 MBS461 H1 trp+ (203, H1), integrated the amyloliquefacience 203 portion around trp locus in the chromosome B. amyloliquefacience 203 Bacillus subtilis 160 "The procedure for the construction of the strain is described in the paper entitled \"Gfenetic defects in DNA repair system and enhancement of intergenote transfoprmation eficiency in Bacillusu subtilis Marburg\" (Mol. gen. Genet.(1978) 162, 229-235). Transformation of strain 15 (trpB160-argA15) with B. amyloliquefaciens 203 DNA. Intergenote trp+ (203, Z5), integrated the amyloliquefacience 203 portion around trp locus in the chromosome." LB medium 48564 MBS462 H5 trp+ (203, H5), integrated the amyloliquefacience 203 portion around trp locus in the chromosome B. amyloliquefacience 203 Bacillus subtilis 160 "The procedure for the construction of the strain is described in the paper entitled \"Gfenetic defects in DNA repair system and enhancement of intergenote transfoprmation eficiency in Bacillusu subtilis Marburg\" (Mol. gen. Genet.(1978) 162, 229-235). Transformation of strain 15 (trpB160-argA15) with B. amyloliquefaciens 203 DNA.Intergenote trp+ (203, Z5), integrated the amyloliquefacience 203 portion around trp locus in the chromosome." LB medium 48564 MBS463 T5 trp+ (203, T5), integrated the amyloliquefacience 203 portion around trp locus in the chromosome B. amyloliquefacience 203 Bacillus subtilis 160 "The procedure for the construction of the strain is described in the paper entitled \"Gfenetic defects in DNA repair system and enhancement of intergenote transfoprmation eficiency in Bacillusu subtilis Marburg\" (Mol. gen. Genet.(1978) 162, 229-235). Transformation of strain 15 (trpB160-argA15) with B. amyloliquefaciens 203 DNA.Intergenote trp+ (203, Z5), integrated the amyloliquefacience 203 portion around trp locus in the chromosome. " LB medium 48564 MBS464 203lys Bacillus amyloliquefacience (formerly B. megaterium) 203 lys "The procedure for the construction of the strain is described in the paper entitled \"Gfenetic defects in DNA repair system and enhancement of intergenote transfoprmation eficiency in Bacillusu subtilis Marburg\" (Mol. gen. Genet.(1978) 162, 229-235). " LB medium 48564 MBS490 203W Bacillus amyloliquefacience (formerly B. megaterium) 203 wild type Hiuga Saito LB medium 48564 MBS465 YAN14618 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔ16-23'S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS466 YAN13642 trpC2 scpB-his6 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS467 YAN12106 trpC2 Δspo0J::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS468 YAN12061 trpC2 Δsmc::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" Modified SMG medium 49533 MBS469 YAN12688 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-7 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS470 YAN12687 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-15 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS471 YAN12583 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-87 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS472 YAN12697 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-144 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS473 YAN12585 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-158 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS474 YAN12590 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-167 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS475 YAN12586 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-175 erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS476 YAN12696 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-(-148) erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS477 YAN12692 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-(-76) erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS478 YAN12693 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-(-15) erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS479 YAN12695 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-(-7) erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS480 YAN12595 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 lysSΩpMrrn-(-3) erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS481 RIK656 trpC2 ΔrrnHG1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::spc rrnO2::kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS482 YAN14396 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 ΔrrnA::kan xynAΩpMrrn-175 erm yoeB::rrn cat::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em 49533 MBS483 YAN12668 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔ16-23-5S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS484 YAN12684 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔ23-5S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS485 YAN12685 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔ23'-5S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS486 YAN12698 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔ5S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS487 YAN12675 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔp rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS488 YAN12700 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 ΔrrnI::ECO/lac rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS489 YAN13492 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::spc ΔICEBs1 Δspo0J::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS491 YAN13940 trpC2 Δspo0J::cat scpB-his6 erm/pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS492 YAN14289 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 scpB-his6 erm/pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS493 YAN13684 trpC2 scpB-his6 erm/pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS495 YAN13983 trpC2 Δsmc::cat scpB-his6 erm/pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc at 25 degrees 49533 MBS496 YAN13904 trpC2 scpB-his6 erm/pRRN/Δp tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS497 YAN13911 trpC2 scpB-his6 erm/pRRN/Δ5S tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS498 YAN13913 trpC2 scpB-his6 erm/pRRN/Δ23-5S tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS499 YAN13914 trpC2 scpB-his6 erm/pRRN/Δ16-23-5S tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS500 YAN14632 trpC2 scpB-his6 erm/pRRN/Δ16-23'S tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS501 YAN14691 trpC2 scpB-his6 erm/pRRN/Δ16-23S tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS502 YAN13981 trpC2 scpB-his6 erm/pECO/lac tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS503 YAN14055 trpC2 scpB-his6 erm/pPARS1 tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS504 YAN14405 trpC2 scpB-his6 erm/pPARS8 tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS505 YAN12644 trpC2 pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS506 YAN12642 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1/ pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS507 YAN12673 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 xynAΩpMrrn-175 erm/ pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em +Tc 49533 MBS508 YAN14669 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnIΔ16-23S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS509 YAN14176 trpC2 yyaC::lacO cat thrC::lacI-gfp mls Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Cm 49533 MBS510 YAN14625 trpC2 Δsmc::cat::spc yyaC::lacO cat thrC::lacI-gfp mls Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" Modified SMG medium +Cm 49533 MBS511 YAN14622 trpC2 Δspo0J::cat::spc yyaC::lacO cat thrC::lacI-gfp mls Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Cm 49533 MBS512 YAN14595 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::kan ΔICEBs1 yyaC::lacO cat thrC::lacI-gfp mls Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Cm 49533 MBS513 YAN14606 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::kan ΔICEBs1 yyaC::lacO cat thrC::lacI-gfp mls Δspo0J::cat::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Cm 49533 MBS514 YAN13122 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 rrnI+ rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533, 74415 MBS515 YAN14041 trpC2 Δsmc::cat::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" Modified SMG medium 49533 MBS516 YAN14243 trpC2 Δspo0J::cat::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS517 YAN13036 trpC2 scpB-mCherry-kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS518 YAN14464 trpC2 scpB-mCherry-kan Δspo0J::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS519 YAN14462 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::spc ΔICEBs1 scpB-mCherry kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS520 YAN14463 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::spc ΔICEBs1 scpB-mCherry kan Δspo0J::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS521 YAN12828 trpC2 ΔrrnA::kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS522 YAN12112 trpC2 yoeB::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS523 YAN14366 trpC2 yoeB::rrnI cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS524 YAN14377 trpC2 yoeB::rrnI cat::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS525 YAN14300 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 ΔrrnA::kan xynAΩpMrrn-175 erm/pRRN tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Em +Tc 49533 MBS526 YAN12205 trpC2 ΔrrnIHG::cat::tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS527 YAN14384 trpC2 ΔrrnIHG::cat::tet ΔtrnI Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS528 YAN13235 trpC2 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnW2 ΔrrnJ1::cat ΔICEBs1 ΔrrnIHG::cat::tet ΔtrnI Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS529 RIK2847 trpC2 ΔrrnI2::catpt1 ΔHG1 Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS530 YAN13938 trpC2 rrnIΔ16-23'S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS531 YAN14640 trpC2 rrnIΔ16-23S rrnG::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium 49533 MBS532 YAN14907 trpC2 /pGETS118-t0-Pr-Sfi-pBR322(lacI-his) tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS533 YAN14908 trpC2 /pRRN(lacI-his) tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" L medium +Tc 49533 MBS534 YAN13568 trpC2 ΔrrnHG1 ΔrrnO1 ΔrrnD1 ΔrrnE1 ΔrrnB2 ΔrrnI2 ΔrrnW2 ΔrrnJ1::spc ΔICEBs1 Δsmc::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Multiple cis-acting rDNAs contribute to nucleoid separation and recruit bacterial condensin, Smc-ScpAB. Cell Reports (2017)\"" Modified SMG medium 49533 MBS535 GEd trpC2, ΔgerE::spc Bacillus subtilis 168 Homologous recombination spectinomycin Sp LB 37°C 50109 MBS536 GEd-5 GEd, amyE::pMF-5'gerE Bacillus subtilis 168 Homologous recombination spectinomycin chloramphenicol Sp,Cm LB 37°C 50109 MBS537 GEd-C GEd, amyE::pMF-gerEBc Bacillus subtilis 168 Homologous recombination spectinomycin chloramphenicol Sp,Cm LB 37°C 50109 MBS538 168-Z trpC2, cotG::pMUTIN-T3, PcotG–lacZr Bacillus subtilis 168 Homologous recombination erythromycin Em LB 37°C 50109 MBS539 GEd-Z GEd, cotG::pMUTIN-T3, PcotG–lacZ 168-Z Bacillus subtilis 168 Homologous recombination spectinomycin erythromycin Sp,Em LB 37°C 50109 MBS540 GEd-5Z GEd-5, cotG::pMUTIN-T3, PcotG–lacZ 168-Z Bacillus subtilis 168 Homologous recombination spectinomycin chloramphenicol erythromycin Sp,Cm,Em LB 37°C 50109 MBS541 GEd-CZ GEd-C, cotG::pMUTIN-T3, PcotG–lacZ 168-Z Bacillus subtilis 168 Homologous recombination spectinomycin chloramphenicol erythromycin Sp,Cm,Em LB 37°C 50109 MBS542 168Gin trpC2, amyE::5'-gerE-gin-gerE-3'r Bacillus subtilis 168 Homologous recombination chloramphenicol Cm LB 37°C 50109 MBS543 GABd 168Gin, ΔgirAB::erm Bacillus subtilis 168 Homologous recombination chloramphenicol erythromycin Cm,Em LB 37°C 50109 MBS544 GCd 168Gin, ΔgirC::kan Bacillus subtilis 168 Homologous recombination chloramphenicol kanamycin Cm,Km LB 37°C 50109 MBS545 GR1 168Gin, Δ(girB–BCE4614)::kan Bacillus subtilis 168 Homologous recombination chloramphenicol kanamycin Cm,Km LB 37°C 50109 MBS546 GR2 168Gin, Δ(BCE4615–BCE4625)::kan Bacillus subtilis 168 Homologous recombination chloramphenicol kanamycin Cm,Km LB 37°C 50109 MBS547 GR3 168Gin, Δ(girB–BCE4619)::kan Bacillus subtilis 168 Homologous recombination chloramphenicol kanamycin Cm,Km LB 37°C 50109 MBS548 GR2X GR2, thrC::girX Bacillus subtilis 168 Homologous recombination chloramphenicol kanamycin erythromycin Cm,Km,Em LB 37°C 50109 MBS549 GC-i 168Gin, Pspac–girC, lacIq, erm Bacillus subtilis 168 Homologous recombination chloramphenicol erythromycin Cm,Em LB 37°C 50109 MBS550 BSIID trpC2, sprB::pMutinT3(PsprB–lacZ, PspoIID–sprB) Bacillus subtilis 168 Homologous recombination erythromycin Em LB 37°C 50108 MBS551 168-AEB trpC2, amyE::spsM(attB for SPβ) Bacillus subtilis 168 Homologous recombination chloramphenicol Cm LB 37°C 50108 MBS552 BSIID-AEB BSIID carrying the amyE::spsM (attB for SPβ) construct Bacillus subtilis 168 Homologous recombination erythromycin chloramphenicol Cm,Em LB 37°C 50108 MBS553 YODUd trpC2, yodU:: pMutinT3(PyodU–lacZ), ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS554 SPRAd trpC2, sprA::pMutinT3(PsprA–lacZ), ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS555 BsINDA trpC2, sprA::pMutinT3(PsprA–lacZ, Pspac–sprA), ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS556 BsINDB trpC2, sprB::pMutinT3(PsprB–lacZ, Pspac–sprB), ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS557 SPless trpC2, attBSPβ; SPβ-cured strain derived from BsINDB Bacillus subtilis 168 SPβ induction by IPTG LB 37°C 50110 MBS558 SPRBd trpC2, ΔsprB:: ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS559 SPmini 168 carrying the minimized SPβ, ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS560 YODUc trpC2, yodU::pMutinT3(PyodU–lacZ), amyE::pMFspsM(spsM) Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS561 SPRAc trpC2, sprA::pMutinT3(PsprA–lacZ), amyE::pMFspsM(spsM) Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS562 CU1050 (SPβ) SPβ-lysogen derived from CU1050 CU1050 SPβ infection LB 37°C 50110 MBS563 BsSPRBG 168 carrying pUBsprBgfp Bacillus subtilis 168 Introduced by electroporation kanamycin Km LB 37°C 50110 MBS564 BsSPSMG trpC2, ypqP–gfp, ermC Bacillus subtilis 168 Homologous recombination Em LB 37°C 50110 MBS565 GERE8G gerE gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis.\"Microbiology. 2004 Jan;150(Pt 1):163-70. " cat LB medium 50390 MBS566 YJCC8G yjcC gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis.\"Microbiology. 2004 Jan;150(Pt 1):163-71." cat LB medium 50390 MBS567 TGL8G tgl gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.\"J Biochem. 2006 May;139(5):887-901. " cat LB medium 50391 MBS568 YCSK8G ycsK gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.\"J Bacteriol. 2007 Mar;189(6):2369-75. Epub 2007 Jan 12." cat LB medium 50392 MBS569 YXEE8G yxeE gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression, localization and modification of YxeE spore coat protein in Bacillus subtilis.\"J Biochem. 2007 Dec;142(6):681-9. Epub 2007 Sep 28." cat LB medium 50393 MBS570 CGEA8G cgeA gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS571 COTA8G cotA gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS572 COTE8G cotE gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS573 COTT8G cotT gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS574 YABG8G yabG gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS575 YEEK8G yeeK gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS576 YHCN8G yhcN gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.\"J Bacteriol. 2009 Feb;191(4):1220-9. doi: 10.1128/JB.01269-08. Epub 2008 Dec 5." cat LB medium 50394 MBS577 COTB8G cotB gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS578 COTC8G cotC gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS579 COTD8G cotD gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS580 COTF8G cotF gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS581 COTZ8G cotZ gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS582 GERQ8G gerQ gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS583 YAAH8G yaaH gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS584 YMAG8G ymaG gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS585 YSND8G ysnD gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS586 YTXO8G ytxO gfp::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Localization of proteins to different layers and regions of Bacillus subtilis spore coats.\"J Bacteriol. 2010 Jan;192(2):518-24. doi: 10.1128/JB.01103-09. Epub 2009 Nov 20." cat LB medium 50395 MBS590 YTFJ8GA2 ytfJ gfp amyE::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The GerW protein is essential for L-alanine-stimulated germination of Bacillus subtilis spores.\"J Biochem. 2013 Nov;154(5):409-17. doi: 10.1093/jb/mvt072. Epub 2013 Aug 6." cat LB medium 50396 MBS591 ASK2001 trpC2 sigM::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS592 ASK2002 trpC2 sigV::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS593 ASK2003 trpC2 sigW::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS594 ASK2004 trpC2 sigX::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS595 ASK2005 trpC2 sigY::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS596 ASK2006 trpC2 sigZ::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS597 ASK2007 trpC2 ylaC::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS598 ASK2008 trpC2 sigI::cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." cat LB medium 53803 MBS599 ASK3200 UOT1285 RM::cat UOT1285 The procedure for the construction of the strain is described in the paper entitled “Inhibitory effect of prophage SPβ fragments on phage SP10 ribonucleotide reductase function and its multiplication in Bacillus subtilis.” Genes Genet Syst. 2011;86(1):7-18. cat LB medium 53804 MBS600 ASK201 trpC2 spoIVCB::erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." erm LB medium 53798 MBS601 ASK202 trpC2 spollGAB::kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." kan LB medium 53798 MBS602 ASK203 trpC2 spoIIIG::kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." kan LB medium 53798 MBS603 ASK204 trpC2 spollAC::kan Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." kan LB medium 53798 MBS604 ASK205 trpC2 spo0H::erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." erm LB medium 53798 MBS605 BSU31 trpC2 amyE::(cat PsigMlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS606 BSU32 trpC2 amyE::(cat PsigVlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS607 BSU33 trpC2 amyE::(cat PsigWlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS608 BSU34 trpC2 amyE::(cat PsigXlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS609 BSU35 trpC2 amyE::(cat PsigYlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS610 BSU36 trpC2 amyE::(cat PsigZlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS611 ASK4400 trpC2 amyE::(cat PyrpGlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Anti-sigma factor-mediated cell surface stress responses in Bacillus subtilis. Genes Genet Syst. 2018 Jan 17. doi: 10.1266/ggs.17-00046. [Epub ahead of print]" cat LB medium 53806 MBS612 BSU37 trpC2 amyE::(cat PylaAlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." cat LB medium 33540 MBS613 BSU62 trpC2 amyE::(cat PylaClacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Transcriptional analysis of the ylaABCD operon of Bacillus subtilis encoding a sigma factor of extracytoplasmic function family.” Genes Genet Syst. 2005 Dec;80(6):385-93." cat LB medium 53800 MBS614 trpC2 amyE::(cat PsigIlacZ) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat LB medium 53801 MBS615 BSU41 trpC2 amyE::PsigM′ (-80 to +296 region of sigM)-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.” FEMS Microbiol Lett. 2004 Mar 12;232(1):93-9." cat LB medium 53799 MBS616 BSU42 trpC2 amyE::PsigW′ (-101 to +155 region of sigW)-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.” FEMS Microbiol Lett. 2004 Mar 12;232(1):93-9." cat LB medium 53799 MBS617 BSU43 trpC2 amyE::PsigX′ (-60 to +245 region of sigX)-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.” FEMS Microbiol Lett. 2004 Mar 12;232(1):93-9." cat LB medium 53799 MBS618 ASK306 trpC2 pDG148 Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS619 ASK313 trpC2 pDG148‐sigM Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS620 ASK310 trpC2 pDG148‐sigV Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS621 ASK314 trpC2 pDG148‐sigW Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS622 ASK315 trpC2 pDG148‐sigX Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS623 ASK312 trpC2 pDG148‐sigZ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS624 ASK311 trpC2 pDG148‐ylaC Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS625 ASK307 trpC2 pDG148‐sigB Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS626 ASK308 trpC2 pDG148‐sigD Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS627 ASK309 trpC2 pDG148‐sigH Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS628 ASK317 trpC2 pDG148‐sigI Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS629 ASK318 trpC2 pDG148‐sigL Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS630 ASK316 trpC2 pDG148‐xpf Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family..” FEMS Microbiol Lett. 2003 Mar 14;220(1):155-60." kan LB medium 33540 MBS631 BSU11 trpC2 sigI :: pMutinT3 Emr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." erm LB medium 53801 MBS632 BSU12 trpC2 rsgI :: pMutinT3 Emr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." erm LB medium 53801 MBS633 BSU13 trpC2 rsgI :: erm Emr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." erm LB medium 53801 MBS634 BSU15 trpC2 amyE :: P sigI -bgaB Cmr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat LB medium 53801 MBS635 BSU16 trpC2 amyE :: P sigI -bgaB rsgI :: pMutinT3 Cmr Emr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat erm LB medium 53801 MBS636 BSU17 trpC2 amyE :: P sigI -bgaB rsgI :: erm Cmr Emr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat erm LB medium 53801 MBS637 BSU18 trpC2 amyE :: P sigI -bgaB sigI :: pMutinT3 Cmr Emr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat erm LB medium 53801 MBS638 BSU19 trpC2 amyE :: P sigI -bgaB sigI :: pMutinT3 rsgI :: pMutinT3 :: spc Cmr Emr Spr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat erm spc LB medium 53801 MBS639 BSU24 trpC2 amyE :: P(-10) sigI -bgaB Cmr Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." cat LB medium 53801 MBS640 BSU26 trpC2 sigI :: erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." erm LB medium 53801 MBS641 BSU27 trpC2 sigI-rsgI :: erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Regulatory role of RsgI in sigI expression in Bacillus subtilis.” Microbiology. 2007 Jan;153(Pt 1):92-101." erm LB medium 53801 MBS642 ASK215 trpC2 spoIIQ-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." cat LB medium 53798 MBS643 ASK216 trpC2 spoIID-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." cat LB medium 53798 MBS644 ASK217 trpC2 sspE-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." cat LB medium 53798 MBS645 ASK218 trpC2 cotA-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.\" J Bacteriol. 1999 Aug;181(15):4584-91." cat LB medium 53798 MBS646 ASK4701 trpC2sigY Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS647 ASK4702 trpC2sigZ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS648 ASK4704 trpC2ylaC Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS649 ASK4730 trpC2sigYsigZ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS650 ASK4731 trpC2sigYylaC Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS651 ASK4732 trpC2ylaCsigZ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS652 ASK4733 trpC2sigYsigZsigV Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS653 ASK4734 trpC2sigYylaCsigV Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS654 ASK4735 trpC2sigYylaCsigZ Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS655 ASK4736 trpC2sigMsigV Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS656 ASK4737 trpC2sigWsigX Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS657 ASK4738 trpC2sigYsigZsigVsigXsigM Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS658 ASK4739 trpC2sigYsigZsigVylaCsigM Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS659 ASK4740 trpC2sigYsigZsigVylaCsigXsigM Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS660 BSU2007 trpC2sigYsigZsigVylaCsigXsigMsigW Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS661 ASK4741 hisHsigYsigZsigVylaCsigXsigMsigW Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.” J Bacteriol. 2008 Apr;190(7):2633-6." LB medium 53803 MBS662 UOT1845 UOT1285 prfB45 UOT1285 "The procedure for the construction of the strain is described in the paper entitled \"Isolation and characterization of sporulation-initiation mutation in the Bacillus subtilis prfB gene.\" Biosci Biotechnol Biochem. 2007 Feb;71(2):397-406. " LB medium 53802 MBS663 RIK10 UOT1285 spo0A(Ps)-bgaB cat UOT1285 "The procedure for the construction of the strain is described in the paper entitled \"ClpC regulates the fate of a sporulation initiation sigma factor, sigmaH protein, in Bacillus subtilis at elevated temperatures.\" Mol Microbiol. 1998 Jul;29(2):505-13." cat LB medium 53797, 74405 MBS664 ASK102 UOT1285 kinA-bgaB cat UOT1285 "The procedure for the construction of the strain is described in the paper entitled \"ClpC regulates the fate of a sporulation initiation sigma factor, sigmaH protein, in Bacillus subtilis at elevated temperatures.\" Mol Microbiol. 1998 Jul;29(2):505-13." cat LB medium 53797 MBS665 RIK50 UOT1285 spo0H-bgaB cat UOT1285 "The procedure for the construction of the strain is described in the paper entitled \"ClpC regulates the fate of a sporulation initiation sigma factor, sigmaH protein, in Bacillus subtilis at elevated temperatures.\" Mol Microbiol. 1998 Jul;29(2):505-13." cat LB medium 53797 MBS666 ASK3000 UOT1285 ΔRM UOT1285 The procedure for the construction of the strain is described in the paper entitled “Inhibitory effect of prophage SPβ fragments on phage SP10 ribonucleotide reductase function and its multiplication in Bacillus subtilis.” Genes Genet Syst. 2011;86(1):7-18. LB medium 53804 MBS667 HRI002 trpC2 ΔcsbB Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " LB medium 53805 MBS668 HRI003 trpC2 ΔyfhO Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " LB medium 53805 MBS669 HRI004 trpC2 ΔcsbB ΔyfhO Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " LB medium 53805 MBS670 HRI005 trpC2 ΔcsbB PsigM'-lacZ cat :: erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " erm LB medium 53805 MBS671 HRI006 trpC2 ΔyfhO PsigM'-lacZ cat :: erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " erm LB medium 53805 MBS672 HRI007 trpC2 ΔcsbB-yfhO PsigM'-lacZ cat :: erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " erm LB medium 53805 MBS673 HRI001 trpC2 amyE :: PsigM'-lacZ cat :: erm Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO.” Biochem Biophys Res Commun. 2013 Jun 21;436(1):6-11. " erm LB medium 53805 MBS674 ASK2070 hisHsigW ybbM::Em (1-112) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Anti-sigma factor-mediated cell surface stress responses in Bacillus subtilis.\" Genes Genet Syst. 2018 Jan 17. doi: 10.1266/ggs.17-00046. [Epub ahead of print]" erm LB medium 53806 MBS675 ASK2071 hisHsigW ybbM::Em (1-160) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Anti-sigma factor-mediated cell surface stress responses in Bacillus subtilis.\" Genes Genet Syst. 2018 Jan 17. doi: 10.1266/ggs.17-00046. [Epub ahead of print]" erm LB medium 53806 MBS676 ASK2072 hisHsigW ybbM::Em (1-178) Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Anti-sigma factor-mediated cell surface stress responses in Bacillus subtilis.\" Genes Genet Syst. 2018 Jan 17. doi: 10.1266/ggs.17-00046. [Epub ahead of print]" erm LB medium 53806 MBS677 Bacillus subtilis (natto) NAFM5 bio-, γPGA+ wild type natto MBS678 Bacillus subtilis (natto) NAFM73 bio-, γPGA-, degQ::erm Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium MBS679 Bacillus subtilis (natto) NAFM79 bio-, γPGA+, amyE::pgsB-lacZ cm Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto)\" (Biosci Biotechnol Biochem. (2009) 73(5),1149-55. The promoter region og pgsB gene was ligated with lacZ and introduced in amyE locus with cm. " cm LB medium 54962 MBS680 Bacillus subtilis (natto) NAFM731 bio-, γPGA+, degQ::erm, sup1 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS681 Bacillus subtilis (natto) NAFM732 bio-, γPGA+, degQ::erm, sup2 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS682 Bacillus subtilis (natto) NAFM733 bio-, γPGA+, degQ::erm, sup3 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS683 Bacillus subtilis (natto) NAFM734 bio-, γPGA+, degQ::erm, sup4 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS684 Bacillus subtilis (natto) NAFM735 bio-, γPGA+, degQ::erm, sup5 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS685 Bacillus subtilis (natto) NAFM736 bio-, γPGA+, degQ::erm, sup6 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS686 Bacillus subtilis (natto) NAFM737 bio-, γPGA+, degQ::erm, sup7 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS687 Bacillus subtilis (natto) NAFM738 bio-, γPGA+, degQ::erm, sup8 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS688 Bacillus subtilis (natto) NAFM739 bio-, γPGA+, degQ::erm, sup9 Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degQ gene was interrupted with erm. " erm LB medium 54963 MBS689 Bacillus subtilis (natto) NAFM104 bio-, γPGA-, degU::km Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The degu gene was interrupted with km. " km LB medium 54963 MBS690 Bacillus subtilis (natto) NAFM114 bio-, γPGA+, yvyE::spc Bacillus subtilis (natto) NAFM5 "The procedure for the construction of the strain is described in the paper entitled \"Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis\" (Applied and Environmental Microbiology. (2011)77, 8249-8258). The yvyE gene was interrupted with spc. " km LB medium 54963 MBS691 Bacillus subtilis (natto) NAFM226 bio-, γPGA+, yabJ::spc "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The yvyE gene was interrupted with spc. The yabJ gene was interupted with spc." spc LB medium 54965 MBS692 Bacillus subtilis (natto) NAFM235 bio-, γPGA-, degQ::erm, yabJ::km, aprE::pyabJ-yabJ(WT) spc "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The genes, degQ, yabJ, and aprE were interrupted with erm, km, and spc, respectively. " erm, km, spc LB medium 54965 MBS693 Bacillus subtilis (natto) NAFM236 bio-, γPGA+, degQ::erm, yabJ::km, aprE::pyabJ-yabJ(S103F) spc "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The genes, degQ, yabJ, and aprE were interrupted with erm, km, and spc, respectively. " erm, km, spc LB medium 54965 MBS694 Bacillus subtilis (natto) NAFM246 bio-, γPGA+, yabJ::km "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The yabJ gene was interrupted with km. " km, LB medium 54965 MBS695 Bacillus subtilis (natto) NAFM250 bio-, γPGA+, yabJ::km, aprE::pyabJ-yabJ(WT) spc "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The genes yabJ and aprE were interrupted with km and spc, respectively. " km, spc LB medium 54965 MBS696 Bacillus subtilis (natto) NAFM251 bio-, γPGA+, yabJ::km, aprE::pyabJ-yabJ(S103F) spc "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The genes yabJ and aprE were interrupted with km and spc, respectively. " km, spc LB medium 54965 MBS697 Bacillus subtilis (natto) NAFM254 bio-, γPGA-, degQ::erm, yabJ::km, aprE::pspac-yabJ(S103F) spc "The procedure for the construction of the strain is described in the paper entitled \"Poly-γ-glutamic acid production of Bacillus subtilis (natto) in the absence of DegQ: A gain-of-function mutation in yabJ gene\" (submitted to Journal of Bioscience and Bioengeneering)The genes, degQ, yabJ, and aprE were interrupted with erm, km, and spc, respectively. " erm, km, spc LB medium 54965 MBS698 Bacillus subtilis (natto) NAFM263 bio-, γPGA+, yueB::erm "The procedure for the construction of the strain is described in the paper entitled \"A Survey of Phage Contamination in Natto-producing Factories and Development of Phage-resistant Bacillus subtilis (natto) Strains\" (Food Science and Technology Research. (2018)24, 485-492). The yueB gene was interrupted with erm. " erm LB medium 54964 MBS699 NBS245 trpC2 ftsA::cat Bacillus subtilis 168 Homologous recombination Cm LB 30°C 59819 MBS700 NBS367 trpC2 aprE::(PftsAZ-gfp-ftsZ cat) Bacillus subtilis 168 Homologous recombination Cm LB 30°C 59819 MBS701 NBS402 trpC2 PftsAZ-ftsA-gfp-cat Bacillus subtilis 168 Homologous recombination Cm LB 30°C 59819 MBS702 NBS800 trpC2 amyE::(Pxyl-gfp-plsX spc) Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS703 NBS1011 trpC2 plsX::pMT3plsX (Pspac-plsX erm) fabD::pfabD15(PrepU-neo-fabD-fabG) PftsAZ-ftsA-gfp cat NBS402 NBS1014 Homologous recombination Em,Nm,Cm LB 30°C 59819 MBS704 NBS1012 trpC2 plsX::pMT3plsX (Pspac-plsX erm) fabD::pfabD15(PrepU-neo-fabD-fabG) aprE::(PftsAZ-gfp-ftsZ cat) NBS367 NBS1014 Homologous recombination Em,Nm,Cm LB 30°C 59819 MBS705 NBS1014 trpC2 plsX::pMT3plsX (Pspac-plsX erm) fabD::pfabD15(PrepU-neo-fabD-fabG) Bacillus subtilis 168 Homologous recombination Em,Nm LB 30°C 59819 MBS706 NBS1327 trpC2 plsX spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS707 NBS1328 trpC2 plsX [D59G] spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS708 NBS1329 trpC2 plsX [L104S] spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS709 NBS1341 trpC2 amyE::(Pxyl-gfp-plsX spc) Pspac-ftsZ erm NBS1330 NBS1008 Homologous recombination Sp,Em LB 30°C 59819 MBS710 NBS1359 trpC2 amyE::(Pxyl-gfp-plsX spc::cat) Bacillus subtilis 168 Homologous recombination Cm LB 30°C 59819 MBS711 NBS1362 trpC2 plsX::Pless-spc amyE::(Pxyl-gfp-plsX spc::cat) Bacillus subtilis 168 Homologous recombination Sp, Cm LB 30°C 59819 MBS712 NBS1365 trpC2 minC::tet amyE::(Pxyl-gfp-plsX spc) NBS1342 NBS800 Homologous recombination Tc, Sp LB 30°C 59819 MBS713 NBS1371 trpC2 ftsA::cat amyE::(Pxyl-gfp-plsX spc) NBS245 NBS800 Homologous recombination Sp, Cm LB 30°C 59819 MBS714 NBS1372 trpC2 plsX spc amyE::(PftsAZ-gfp-ftsZ cat) NBS1327 NBS367 Homologous recombination Sp, Cm LB 30°C 59819 MBS715 NBS1374 trpC2 plsX spc, PftsAZ-ftsA-gfp-cat NBS1327 NBS402 Homologous recombination Sp, Cm LB 30°C 59819 MBS716 NBS1375 trpC2 plsX103 [D59G, L104S] spc PftsAZ-ftsA-gfp cat NBS1010 NBS402 Homologous recombination Sp, Cm LB 30°C 59819 MBS717 NBS1398 plsC-C△7 spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS718 NBS1399 plsC spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS719 NBS1517 trpC2 plsX-his12 spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS720 NBS1569 trpC2 thrC::(Phy-spank-ftsA erm) Bacillus subtilis 168 Homologous recombination Em LB 30°C 59819 MBS721 NBS1571 trpC2 thrC::(Phy-spank-mciZ erm) CU1050 Homologous recombination Em LB 30°C 59819 MBS722 NBS1572 trpC2 thrC::(Phy-spank-sirA erm) Bacillus subtilis 168 Introduced by electroporation Em LB 30°C 59819 MBS723 NBS1578 trpC2 thrC::(Phy-spank-ftsA erm) spec-gfp-ftsA NBS1569 NBS1576 Homologous recombination Em, Sp LB 30°C 59819 MBS724 NBS1579 trpC2 thrC::(Phy-spank-ftsA erm) amyE::(Pxyl-gfp-plsX spc) NBS1569 NBS800 Homologous recombination Em, Sp LB 30°C 59819 MBS725 NBS1875 trpC2 thrC::(Phy-spank-mciZ erm) amyE::(Pxyl-gfp-plsX spc) minCD::tet NBS1342 NBS1583 Homologous recombination Em, Sp, Tc LB 30°C 59819 MBS726 NBS1876 trpC2 amyE::(Pxyl-gfpA206K-plsX spc) Homologous recombination Sp LB 30°C 59819 MBS727 NBS1877 trpC2 thrC::(Phy-spank-gfp-plsX erm) Homologous recombination Em LB 30°C 59819 MBS728 NBS1880 trpC2 spec-cfp(Bs)-ftsA amyE::(Pxyl-gfp-plsX spc::cat) thrC::(Phy-spanK-ftsA erm) NBS1569 NBS1879 Homologous recombination Sp, Cm, Em LB 30°C 59819 MBS729 NBS801 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE Homologous recombination Sp LB 37°C 59820 MBS730 NBS2648 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE comK::tet Homologous recombination Sp Cm LB 37°C 59820 MBS731 NBS2649 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE comP::tet Homologous recombination Sp Tc LB 37°C 59820 MBS732 NBS2650 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE comA::tet Homologous recombination Sp Tc LB 37°C 59820 MBS733 NBS2651 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE rok::tet Homologous recombination Sp Tc LB 37°C 59820 MBS734 NBS2652 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA::tet Homologous recombination Sp Tc LB 37°C 59820 MBS735 NBS2653 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recO::erm Homologous recombination Sp Em LB 37°C 59820 MBS736 NBS2654 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recU::erm Homologous recombination Sp Em LB 37°C 59820 MBS737 NBS2655 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA K70R-erm Homologous recombination Sp Em LB 37°C 59820 MBS738 NBS2656 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA R58C-erm Homologous recombination Sp Em LB 37°C 59820 MBS739 NBS2657 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA E154R-erm Homologous recombination Sp Em LB 37°C 59820 MBS740 NBS2658 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA E154V-erm Homologous recombination Sp Em LB 37°C 59820 MBS741 NBS2659 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA G155P-erm Homologous recombination Sp Em LB 37°C 59820 MBS742 NBS2660 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA G155R-erm Homologous recombination Sp Em LB 37°C 59820 MBS743 NBS2661 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA D159A-erm Homologous recombination Sp Em LB 37°C 59820 MBS744 NBS2662 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA G202I-erm Homologous recombination Sp Em LB 37°C 59820 MBS745 NBS2663 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA F215Q-erm Homologous recombination Sp Em LB 37°C 59820 MBS746 NBS2664 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA K241Q K243N-erm Homologous recombination Sp Em LB 37°C 59820 MBS747 NBS2665 trpC2 amyE'::(Phyper-spank IS256Bsu1 tnp IRL-cat-IRR spc)::'amyE recA::muB-tet Homologous recombination Sp Tc LB 37°C 59820 MBS861 NBS1440 trpC2 relA::erm yjbM::tet ywaC::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Spc/Tet LB medium 37°C 60839 MBS862 NBS2408 trpC2 relA::erm ΔyjbM ΔywaC Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1 Em LB medium 37°C 60839 MBS863 NBS2396 trpC2 relA::erm yjbM::tet ywaC::spc prs913C>T-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em/Spc/tet LB medium 37°C 60839 MBS864 NBS2397 trpC2 relA::erm yjbM::tet ywaC::spc prs443T>G-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em/Spc/tet LB medium 37°C 60839 MBS865 NBS2895 trpC2 relA::erm yjbM::tet ywaC::spc gmk104A>C-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em/Spc/tet LB medium 37°C 60839 MBS866 NBS2391 trpC2 relA::erm yjbM::tet ywaC::spc hprT209A>G-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em/Spc/tet LB medium 37°C 60839 MBS867 NBS2464 trpC2 relA::erm yjbM::tet ywaC::spc purF32A>G-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em/Spc/tet LB medium 37°C 60839 MBS868 NBS3474 trpC2 ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5 Cm LB medium 37°C 60839 MBS869 NBS3475 trpC2 rpoC1276T>C-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5 Cm LB medium 37°C 60839 MBS870 NBS3476 trpC2 rpoC968A>G-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5 Cm LB medium 37°C 60839 MBS871 NBS3477 trpC2 relA::erm ΔyjbM ΔywaC ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em LB medium 37°C 60839 MBS872 NBS3478 trpC2 relA::erm ΔyjbM ΔywaC rpoC1276T>C-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em LB medium 37°C 60839 MBS873 NBS3479 trpC2 relA::erm ΔyjbM ΔywaC rpoC968A>G-cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Em1 Cm/Em LB medium 37°C 60839 MBS874 NBS2337 trpC2 codY::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Spc100 Spc LB medium 37°C 60839 MBS875 NBS3486 trpC2 relA::erm ΔyjbM ΔywaC codY::spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em/Spc100 Em/Spc LB medium 37°C 60839 MBS876 NBS3487 trpC2 relA::erm ΔyjbM ΔywaC codY::spc ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Spc100 Cm/Spc/Em LB medium 37°C 60839 MBS877 NBS2878 trpC2 guaB::tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Tet15 Tet LB medium 37°C 60839 MBS878 NBS3480 trpC2 guaB416T>C-spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Spc100 Spc LB medium 37°C 60839 MBS879 NBS3481 trpC2 guaB362C>T-spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Spc100 Spc LB medium 37°C 60839 MBS880 NBS3493 trpC2 guaB586A>G-spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Spc100 Spc LB medium 37°C 60839 MBS881 NBS3482 trpC2 relA::erm ΔyjbM ΔywaC guaB416T>C-spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Spc LB medium 37°C 60839 MBS882 NBS3483 trpC2 relA::erm ΔyjbM ΔywaC guaB362C>T-spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Spc LB medium 37°C 60839 MBS883 NBS3494 trpC2 relA::erm ΔyjbM ΔywaC guaB586A>G-spc Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Spc LB medium 37°C 60839 MBS884 NBS3484 trpC2 relA::erm ΔyjbM ΔywaC guaB416T>C-spc ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Cm/Spc LB medium 37°C 60839 MBS885 NBS3485 trpC2 relA::erm ΔyjbM ΔywaC guaB362C>T-spc ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Cm/Spc LB medium 37°C 60839 MBS886 NBS3495 trpC2 relA::erm ΔyjbM ΔywaC guaB586A>G-spc ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Em1/Spc100 Em/Cm/Spc LB medium 37°C 60839 MBS887 NBS3496 pDL2 trpC2 amyE::PyitJ-lacZ cat::tet Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Tet15 Tet LB medium 37°C 60839 MBS888 NBS3497 pDL2 trpC2 amyE::PyitJ-lacZ cat::tet relA::erm ΔyjbM ΔywaC ybxB::cat-rpoB1865C>T Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5/Tet15 Cm/Em/Tet LB medium 37°C 60839 MBS889 NBS3498 pDL2 trpC2 amyE::PyitJ-lacZ cat Bacillus subtilis 168 "The procedure for the construction of the strain is described in the paper entitled \"Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. \"(Mol. Microbiol.(2020), in press)" Cm5 Cm LB medium 37°C 60839 MBS749 BEST386 pHY300PLK leuB8 arg-15 hsdRM Bacillus subtilis 168 tet tet LB medium 37°C 61169 MBS750 BEST387 pHY300PLK leuB8 arg-15 hsdRM Bacillus subtilis 168 tet tet LB medium 37°C 61169 MBS751 BEST40595 pUB110 leuB8 arg-15 hsdRM Bacillus subtilis 168 kan phl kan LB medium 37°C 61169 MBS752 BEST23124 pLS20hyg leuB8 arg-15 hsdRM Bacillus subtilis 168 hyg kan LB medium 37°C 61169 MBS753 BEST4301 proB::pBRoriN32 yjcI::ne-spc pycA::eo-bsr Bacillus subtilis 168 SmR, BsR, SpcR LB medium 37°C 61169 MBS754 BEST4302 xkdE::oriT110-stm unit yjcI::ne-spc, pycA::eo-bsr Bacillus subtilis 168 SmR, BsR, SpcR LB medium 37°C 61169 MBS755 BEST4305 pLS20hyg BEST4301 plus pLS20hyg Bacillus subtilis 168 SmR, BsR, SpcR, HmR LB medium 37°C 61169 MBS756 BEST4306 pLS20hyg BEST4302 plus pLS20hyg Bacillus subtilis 168 SmR, BsR, SpcR, HmR LB medium 37°C 61169 MBS757 BEST6621 oriT110-stm Bacillus subtilis 168 SmR SpcR LB medium 37°C 61170 MBS758 BEST6627 pLS20hyg oriT110-stm plus pLS20hyg Bacillus subtilis 168 HmR SmR SpcR LB medium 37°C 61170 MBS759 BEST6623 oriT110-stm Bacillus subtilis 168 SmR BSR LB medium 37°C 61170 MBS760 BEST6629 pLS20hyg oriT110-stm plus pLS20hyg Bacillus subtilis 168 HmR SmR BSR LB medium 37°C 61170 MBS761 BEST6625 oriT110-stm Bacillus subtilis 168 SmR BSR SpcR LB medium 37°C 61170 MBS762 BEST6631 pLS20hyg oriT110-stm plus pLS20hyg Bacillus subtilis 168 HmR SmR BSR SpcR LB medium 37°C 61170 MBS763 BEST7817 oriT110-stm Bacillus subtilis 168 SmR SpcR EmR LB medium 37°C 61170 MBS764 BEST7821 oriT110-stm Bacillus subtilis 168 SmR SpcR EmR LB medium 37°C 61170 MBS766 BEST7819 oriT110-stm Bacillus subtilis 168 SmR SpcR EmR LB medium 37°C 61170 MBS767 BEST7834 pLS20hyg oriT110-stm plus pLS20hyg Bacillus subtilis 168 HmR SmR SpcR EmR LB medium 37°C 61170 MBS768 RM125 leuB8 arg-15 hsdRM Bacillus subtilis 168 LB medium 37°C 61169 MBS769 BEST215 pBSVG101 trpC2 met::pBREm Bacillus subtilis 168 TcR LB medium 37°C 61162 MBS770 BEST3156 pBSVG104 trpC2 met::pBREm Bacillus subtilis 168 CmR LB medium 37°C 61162 MBS771 BEST40401 pLS20cat leuB8 arg-15 hsdRM Bacillus subtilis 168 LB medium 37°C 61164 MBS772 BEST2125 trpC2 proB::pBRTc Bacillus subtilis 168 TcR LB medium 37°C 61164 MBS773 BEST8630 trpC2 recA362::tet Bacillus subtilis 168 TcR LB medium 37°C 61164 MBS774 CU741 leuC7 trpC2 Bacillus subtilis 168 LB medium 37°C 61167 MBS775 BEST3074 CU741 plus acoL::neo Bacillus subtilis 168 NmR LB medium 37°C 61167 MBS776 BEST3075 CU741 plus acoL::I luB::tet Bacillus subtilis 168 TcR LB medium 37°C 61167 MBS777 BEST3076 CU741 plus acoL::I luB::tet Bacillus subtilis 168 NmR LB medium 37°C 61167 MBS778 BEST3077 Bacillus subtilis 168 TcR LB medium 37°C 61167 MBS779 BEST3084 Bacillus subtilis 168 NmR LB medium 37°C 61167 MBS780 BEST3085 Bacillus subtilis 168 TcR LB medium 37°C 61167 MBS781 BEST3087 Bacillus subtilis 168 TcR LB medium 37°C 61167 MBS782 BEST3088 Bacillus subtilis 168 NmR LB medium 37°C 61167 MBS783 BEST3091 Bacillus subtilis 168 BsR LB medium 37°C 61167 MBS784 OA101 prototroph Bacillus subtilis 168 LB medium 37°C 61172 MBS785 BEST3095 Bacillus subtilis 168 CmR, NmR, TcR LB medium 37°C 61172 MBS786 BEST3096 Bacillus subtilis 168 CmR LB medium 37°C 61172 MBS787 BEST3102 Bacillus subtilis 168 LB medium 37°C 61172 MBS788 BEST3106 Bacillus subtilis 168 LB medium 37°C 61172 MBS789 BEST3109 Bacillus subtilis 168 LB medium 37°C 61172 MBS790 BEST3125 Bacillus subtilis 168 TcR LB medium 37°C 61172 MBS791 BEST3136 Bacillus subtilis 168 NmR LB medium 37°C 61172 MBS792 BEST3145 Bacillus subtilis 168 NmR、TcR LB medium 37°C 61172 MBS793 BEST195 Bacillus subtilis natto (BEST195) LB medium 37°C 61172 MBS794 BEST21274 pLS20cat leu arg hsdRM Δ(yvfC-yveP)::pr-neo Bacillus subtilis 168 NmR, CmR LB medium 37°C 61165 MBS795 BEST21278 pLSGETS101spc leu, arg, hsdRM Δ(yvfC-yveP)::pr-neo Bacillus subtilis 168 SpcR LB medium 37°C 61165 MBS796 BEST21290 pLSGETS101spc leu arg hsdRM Δ(yvfC-yveP)::pr-neo proB::pBRTc Bacillus subtilis 168 TcR, SpcR, NmS LB medium 37°C 61165 MBS797 BEST21292 pLSGETS103tet hyper leu, arg, hsdRM Δ(yvfC-yveP)::pr-neo proB::pBRTc TetR, NmR, SpecS, CmR [CReT] BEST21290 Bacillus subtilis 168 TcR NmR SpcS LB medium 37°C 61165 MBS798 BEST21299 pLSGETS101spc trpC2 Bacillus subtilis 168 SpcR, CmR LB medium 37°C 61165 MBS799 BEST21300 pLSGETS103tet trpC2 Bacillus subtilis 168 TcR, CmR LB medium 37°C 61165 MBS800 BEST21317 pLSGETS101spc trpC2 yjcI::cat Bacillus subtilis 168 SpcR, CmR LB medium 37°C 61165 MBS801 BEST21305 pLSGETS2001 hyper leu arg hsdRM proB::pBR(25.7 kb: spc) Bacillus subtilis 168 SpcR, CmR, TcS LB medium 37°C 61165 MBS802 BEST21320 pLSGETS2002 hyper leu arg hsdRM proB::pBR(39.7 kb: spc) Bacillus subtilis 168 SpcR, CmR, TcS LB medium 37°C 61165 MBS803 BEST21322 pLSGETS2003 hyper leu arg hsdRM proB::pBR(54.2 kb: spc) Bacillus subtilis 168 SpcR, CmR, TcS LB medium 37°C 61165 MBS804 BEST21335 pLSGETS2004 hyper leu arg hsdRM proB::pBR(90.3 kb: spc) Bacillus subtilis 168 SpcR, CmR, TcS LB medium 37°C 61165 MBS805 BEST21309 trpC2 leuB::pBRTc proB::pBRBS yjcI::pBREm leuB::neo Bacillus subtilis 168 LB medium 37°C 61165 MBS806 BEST2137 trpC2 leuB::pBRTc proB::pBRBS yjcI::pBREm leuB::neo Bacillus subtilis 168 TcR, NmR LB medium 37°C 61165 MBS807 BEST21434 trpC2 [3]::te+ [8]::et pBEAZ191 pBEAZ195 168 Bacillus subtilis 168 PhlR, EmR LB medium 37°C 61166 MBS814 BEST21437 trpC2 inv[3-8]T Bacillus subtilis 168 TcR LB medium 37°C 61166 MBS815 BEST21465 trpC2 inv[3-8]T [1]::eo+, [4]::ne- Bacillus subtilis 168 TcR LB medium 37°C 61166 MBS816 BEST21503 trpC2 inv[3-8]T [2]::eo+, [4]::ne- Bacillus subtilis 168 TcR LB medium 37°C 61166 MBS817 BEST21593 trpC2 inv[1-4]N, [3]::te- [8]::et+ Bacillus subtilis 168 NmR LB medium 37°C 61166 MBS818 BEST21575 trpC2 inv[2-4]N, [3]::te- [8]::et+ Bacillus subtilis 168 NmR LB medium 37°C 61166 MBS819 BEST21474 trpC2 inv[3-8]T/[1-4]N Bacillus subtilis 168 NmR, TcR LB medium 37°C 61166 MBS820 BEST21524 trpC2 inv[3-8]T/[2 -4]N Bacillus subtilis 168 NmR, TcR LB medium 37°C 61166 MBS822 BEST21581 trpC2 inv[2-4]N/[3-8]T Bacillus subtilis 168 NmR, TcR LB medium 37°C 61166 MBS823 BEST3015 OA101 based Bacillus subtilis 168 CmR LB medium 37°C 61161 MBS824 BEST3028 OA101 based Bacillus subtilis 168 CmR LB medium 37°C 61161 MBS825 BEST3055 OA101 based Bacillus subtilis 168 CmR、NmR, BsR LB medium 37°C 61161 MBS826 BEST4041 CU741-based Bacillus subtilis 168 CmR LB medium 37°C 61161 MBS827 BEST4087 CU741-based Bacillus subtilis 168 CmR LB medium 37°C 61161 MBS828 BEST4133 CU741-based Bacillus subtilis 168 CmR、NmR, BsR LB medium 37°C 61161 MBS829 BEST9405 metB51 Bacillus subtilis 168 BsR, ts LB medium 37°C 61171 MBS830 BEST9412 metB51 Bacillus subtilis 168 BsR, ts, SmR LB medium 37°C 61171 MBS831 BEST9410 trpC2-based Bacillus subtilis 168 BsR, ts LB medium 37°C 61171 MBS832 BEST9413 trpC2-based Bacillus subtilis 168 BsR, ts, SmR LB medium 37°C 61171 MBS833 BEST9416 trpC2-based Bacillus subtilis 168 GFP LB medium 37°C 61171 MBS834 BEST9411 leu arg Bacillus subtilis 168 BsR, ts LB medium 37°C 61171 MBS835 BEST9414 leu arg Bacillus subtilis 168 BsR, ts, SmR LB medium 37°C 61171 MBS836 BEST9417 leu arg Bacillus subtilis 168 GFP LB medium 37°C 61171 MBS837 BEST9418 leu arg recA::cat Bacillus subtilis 168 CmR, MMCS, GFP LB medium 37°C 61171 MBS838 BEST9419 leu arg recA362::tet Bacillus subtilis 168 TcR, MMCS, GFP LB medium 37°C 61171 MBS839 BEST9421 leu arg recA4 Bacillus subtilis 168 MMCS, GFP LB medium 37°C 61171 MBS840 BEST40875 pLSBAC101 Bacillus subtilis RM125 CmR SpR LB medium 37°C 61168 MBS841 BEST41001 proB::pBR[BAC-1]cat Bacillus subtilis RM125 CmR SpS LB medium 37°C 61168 MBS842 BEST41002 proB::pBR[BAC-2]cat Bacillus subtilis RM125 CmR SpS LB medium 37°C 61168 MBS843 BEST41003 proB::pBR[BAC-3]cat Bacillus subtilis RM125 CmR SpS LB medium 37°C 61168 MBS844 BEST41004 proB::pBR[BAC-4]cat Bacillus subtilis RM125 CmR SpS LB medium 37°C 61168 MBS845 BEST41005 proB::pBR[BAC-1]erm Bacillus subtilis RM125 CmS EmR SpR LB medium 37°C 61168 MBS846 BEST41006 proB::pBRtet[BAC-1]erm Bacillus subtilis RM125 TcR EmR SpR LB medium 37°C 61168 MBS847 BEST41007 proB::pBR[BAC-3]erm Bacillus subtilis RM125 CmS EmR SpR LB medium 37°C 61168 MBS848 BEST41008 proB::pBRtet[BAC-1+2]cat Bacillus subtilis RM125 TcR CmR EmS SpS LB medium 37°C 61168 MBS849 BEST41009 proB::pBRtet[BAC-1+2+3]erm Bacillus subtilis RM125 TcR CmS EmR SpR LB medium 37°C 61168 MBS850 BEST41024 pLSBAC(1) Bacillus subtilis RM125 TcR CmR EmS LB medium 37°C 61168 MBS851 BEST41025 pLSBAC(1+2) Bacillus subtilis RM125 TcR CmR EmS LB medium 37°C 61168 MBS902 BEST7003 Bacillus subtilis RM125 TcR、NmR LB medium 37°C 61163 MBS903 BEST7031 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS904 BEST7053 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS905 BEST7078 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS906 BEST7091 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS907 BEST7101 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS908 BEST7116 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS909 BEST7143 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS910 BEST7152 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS911 BEST7155 Bacillus subtilis RM125 NmR LB medium 37°C 61163 MBS912 BEST7324 Bacillus subtilis RM125 BsR LB medium 37°C 61163 MBS913 BEST7328 Bacillus subtilis RM125 CmR、BsR LB medium 37°C 61163 MBS914 BEST7337 Bacillus subtilis RM125 EmR, BsR LB medium 37°C 61163 MBS915 BEST7341 Bacillus subtilis RM125 CmR、BsR LB medium 37°C 61163 MBS916 BEST7345 Bacillus subtilis RM125 EmR, BsR LB medium 37°C 61163 MBS917 BEST7349 Bacillus subtilis RM125 CmR, BsR LB medium 37°C 61163 MBS918 BEST7352 Bacillus subtilis RM125 EmR, BsR LB medium 37°C 61163 MBS919 BEST7360 Bacillus subtilis RM125 EmR, BsR LB medium 37°C 61163 MBS920 BEST7374 Bacillus subtilis RM125 CmR、NmS, BsR LB medium 37°C 61163 MBS921 BEST7527 Bacillus subtilis RM125 CmS、NmR, BsR LB medium 37°C 61163 MBS922 BEST7532 Bacillus subtilis RM125 CmS、NmR, BsR LB medium 37°C 61163 MBS923 BEST7534 Bacillus subtilis RM125 CmR、NmR, BsR LB medium 37°C 61163 MBS924 BEST7538 Bacillus subtilis RM125 CmS、NmR, BsR LB medium 37°C 61163 MBS925 BEST7544 Bacillus subtilis RM125 CmR、NmR, BsR LB medium 37°C 61163 MBS926 BEST7546 Bacillus subtilis RM125 CmS、NmR, BsR LB medium 37°C 61163 MBS927 BEST7552 Bacillus subtilis RM125 CmR、NmR, BsR LB medium 37°C 61163 MBS928 BEST7562 Bacillus subtilis RM125 CmR、NmR, BsR LB medium 37°C 61163 MBS929 BEST7566 Bacillus subtilis RM125 CmS、NmR, BsR LB medium 37°C 61163 MBS930 BEST7488 Bacillus subtilis RM125 CmS、NmS, BsR LB medium 37°C 61163 MBS931 BEST7491 Bacillus subtilis RM125 CmR、NmS, BsR LB medium 37°C 61163 MBS932 BEST7497 Bacillus subtilis RM125 CmR、NmS, BsR LB medium 37°C 61163 MBS933 BEST7571 Bacillus subtilis RM125 CmS、NmS, BsR LB medium 37°C 61163 MBS934 BEST7574 Bacillus subtilis RM125 CmR、NmS, BsR LB medium 37°C 61163 MBS935 BEST7605 Bacillus subtilis RM125 CmS、NmR, BsR LB medium 37°C 61163 MBS936 BEST7613 Bacillus subtilis RM125 CmR、NmR, BsR LB medium 30°C 61163 MBS890 NBS1009 plsX::pMHAcPLSX (plsX-HA erm-Pspac-fabD-fabG) Bacillus subtilis 168 Homologous recombination Em, IPTG LB 30°C 59819 MBS891 NBS1010 trpC2 plsX103 [D59G, L104S] spc Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS892 NBS1330 trpC2 Pspac-ftsZ erm Bacillus subtilis 168 Homologous recombination Em, IPTG LB 30°C 59819 MBS893 NBS1342 minCD::tet Bacillus subtilis 168 Homologous recombination Tc LB 30°C 59819 MBS894 NBS1373 trpC2 plsX103 [D59G, L104S] spc aprE::(PftsAZ-gfp-ftsZ cat) NBS1010 NBS367 Homologous recombination Sp, Cm LB 30°C 59819 MBS895 NBS1576 spec-gfp-ftsA Bacillus subtilis 168 Homologous recombination Sp LB 30°C 59819 MBS896 NBS1583 trpC2 thrC::(Phy-spank-mciZ erm) amyE::(Pxyl-gfp-plsX spc) NBS1571 NBS800 Homologous recombination Em, Sp LB 30°C 59819 MBS897 NBS1584 trpC2 thrC::(Phy-spank-sirA erm) spec-gfp-ftsA NBS1572 NBS1576 Homologous recombination Em, Sp LB 30°C 59819 MBS898 NBS1585 trpC2 thrC::(Phy-spank-sirA erm) amyE::(Pxyl-gfp-plsX spc) NBS1572 NBS800 Homologous recombination Em, Sp LB 30°C 59819 MBS899 NBS1878 trpC2 spec-cfp(Bs)-ftsA Homologous recombination Sp LB 30°C 59819 MBS900 NBS1879 trpC2 spec-cfp(Bs)-ftsA amyE::(Pxyl-gfp-plsX spc::cat) NBS1878 NBS800 Homologous recombination Sp, Cm LB 30°C 59819 MBS901 NBS1881 trpC2 spec-cfp(Bs)-ftsA amyE::(Pxyl-gfp-plsX spc::cat) minCD::tet NBS1342 NBS1879 Homologous recombination Sp, Cm, Tc LB 30°C 59819 MBS937 NEST105 pHY300PLK, pLS30 Bacillus subtilis natto (BEST195) TcR 70265 MBS938 NEST116 pHY300PLK, pLS30, pLS20cat Bacillus subtilis natto (BEST195) CmR, TcR 70265 MBS939 NEST121 pHY300PLK, pLS30, pLS20hyg Bacillus subtilis natto (BEST195) HmR, TcR 70265 MBS940 NEST125 pHY300PLK, pLS30, pLS20neogfp Bacillus subtilis natto (BEST195) NmR, TcR 70265 MBS941 BEST40483 pLS20neo Bacillus subtilis RM125 NmR 70265 MBS942 BEST40503 pLS20neogfp Bacillus subtilis RM125 NmR 70265 MBS943 BEST40715 pLS20hyg Bacillus subtilis RM125 HmR 70265 MBS944 BEST2213 Bacillus subtilis 168 NmR SpR 70261 MBS945 BEST2215 Bacillus subtilis 168 NmR SpR 70261 MBS946 BEST2216 Bacillus subtilis 168 NmR SpR 70261 MBS947 BEST2217 Bacillus subtilis 168 NmR SpR 70261 MBS948 BEST2218 Bacillus subtilis 168 NmR SpR 70261 MBS949 BEST2219 Bacillus subtilis 168 NmR SpR 70261 MBS950 BEST2220 Bacillus subtilis 168 NmR SpR 70261 MBS951 BEST2221 Bacillus subtilis 168 NmR SpR 70261 MBS952 BEST23 rnhC151::cat CU741 Bacillus subtilis 168 CmR 70264 MBS953 BEST138 rnhB21::neo OA101 Bacillus subtilis 168 NmR 70264 MBS954 BEST206 ypdQ44::spc Bacillus subtilis 168 SpR 70264 MBS955 BEST218 rnhB21::neo Bacillus subtilis 168 NmR 70264 MBS956 BEST220 rnhC151::cat Bacillus subtilis 168 CmR 70264 MBS959 BEST2131 leuB::pBRTc Bacillus subtilis 168 TcR 70262 MBS960 BEST2007 proB::pBRCm Bacillus subtilis 168 CmR 70262 MBS961 BEST2012 proB::pBREm/Cm leu+ Bacillus subtilis 168 CmR EmR 70262 MBS962 BEST2037 proB::16.8Em leu+ Bacillus subtilis 168 EmR 70262 MBS963 BEST2045 proB::39.5Em/Cm leu+ Bacillus subtilis 168 CmR EmR 70262 MBS964 BEST2046 proB::48.5Em leu+ Bacillus subtilis 168 EmR 70262 MBS965 BEST2204 proB::pBREm/Cm leuB::tet Bacillus subtilis 168 CmR EmR TcR 70262 MBS966 BEST2207 proB::16.8EmR leuB::tet Bacillus subtilis 168 EmR TcR 70262 MBS967 BEST2042 proB::26.2Em/Cm leuB::tet Bacillus subtilis 168 CmR EmR TcR 70262 MBS968 BEST2205 proB::39.5Em/Cm leuB::tet Bacillus subtilis 168 CmR EmR TcR 70262 MBS969 BEST2206 proB::48.5Em leuB::tet Bacillus subtilis 168 EmR TcR 70262 MBS971 BEST2208 pro+ leuB::26.2Em/Cm Bacillus subtilis 168 CmR EmR 70262 MBS972 BEST2210 pro+ leuB::39.5Em/Cm Bacillus subtilis 168 CmR EmR 70262 MBS973 BEST2209 proB::26.2Em/Cm leuB::26.2Em/Cm Bacillus subtilis 168 CmR EmR 70262 MBS974 NBS1001 trpC2 Δtig::Cm Bacillus subtilis 168 Homologous recombination Cm LB 37°C 70266 MBS975 NBS1001S trpC2 Δtig::Spc Bacillus subtilis 168 Homologous recombination Sp LB 37°C Distribution is suspended because the strain is not described in the original paper.
If you would like to request, please contact us. 70266 MBS976 NBS1002 trpC2 ΔsigB::Em Bacillus subtilis 168 Homologous recombination Em LB 37°C 70266 MBS977 NBS2000 trpC2 ΔdnaK::Spc Bacillus subtilis 168 Homologous recombination Sp LB 37°C 70266 MBS978 NBS2001 trpC2 ΔdnaK-dnaJ::Spc Bacillus subtilis 168 Homologous recombination Sp LB 37°C 70266 MBS979 NBS2002 trpC2 ΔdnaK-dnaJ::Spc Δtig::Cm Bacillus subtilis 168 Homologous recombination Sp, Cm LB 37°C 70266 MBS980 NBS2003 trpC2 ΔgrpE-dnaK-dnaJ::Cm Bacillus subtilis 168 Homologous recombination Cm LB 37°C 70266 MBS981 NBS2004 trpC2 ΔgrpE-dnaK-dnaJ::Cm Δtig::Spc Bacillus subtilis 168 Homologous recombination Cm, Sp LB 37°C 70266 MBS982 NBS2005 trpC2 ΔdnaK-dnaJ::Spc ΔsigB::Em Bacillus subtilis 168 Homologous recombination Sp, Em LB 37°C 70266 MBS983 NBS2006 trpC2 ΔgrpE-dnaK-dnaJ::Spc ΔsigB::Em Bacillus subtilis 168 Homologous recombination Sp, Em LB 37°C 70266 MBS989 BEST23247 pLS20tshb arg, leu HmR, BSR, ts L medium 30°C 72351 MBS990 NEST130 pHY300PLK, pLS30, pLS20tshb pro BEST23247 [CT] NEST116 TcR, HmR, BSR, ts L medium 30°C 72351 MBS991 NEST134 pHY300PLK, pLS30 pro spontaneous loss of pLS20tshb TcR L medium 37°C 72351 MBS992 NEST136 pHY300PLK, pLS3001, pLS20cat, pLS30 pro BEST40411 [CT] NEST134 TcR, BSR, SpR, CmR L medium 37°C 72351 MBS993 NEST138 pLS20cat pro spontaneous loss of pHY300PLK, pLS3001, pLS30 CmR L medium 37°C 72351 MBS994 NEST140 pLS20tshb pro BEST23247 [CT] NEST138 BSR, HmR L medium 30°C 72351 MBS995 NEST141 pro spontaneous loss of pLS20tshb L medium 37°C 72351 MBS996 NEST150 pUB110 pro BEST40402 [CT] NEST141 KmR L medium 37°C 72351 MBS997 NEST151 pUB110, pLS20cat pro BEST40402 [CT] NEST141 KmR, CmR L medium 37°C 72351 MBS998 NEST152 pLS3001 pro BEST40411 [CT] NEST141 BSR, SpR, CmR L medium 37°C 72351 MBS999 NEST153 pLS3001, pLS20cat pro BEST40411 [CT] NEST141 BSR, SpR, CmR L medium 37°C 72351 MBS1000 NEST154 pLS20neogfp pro BEST40503 [CT] NEST141 NmR, Gfp L medium 37°C 72351 MBS1002 BEST377 leuB8 arg-15 hsdRM xkdE::pLS20coreH(HmR) Bacillus subtilis RM125 hygromycin resistance (HmR, 100 μg/mL) 80650 MBS1003 BEST378 leuB8 arg-15 hsdRM xkdE::pLS20coreH(HmR) Bacillus subtilis RM125 hygromycin resistance (HmR, 100 μg/mL) The orientation of pLS20coreH in BEST378 is opposite to that in BEST377. 80650 MBS1004 BESN1008 leuB8 arg-15 hsdRM xkdE::pLS20coreH(HmR) pycA::eo-bsr yjcI::ne-spc Bacillus subtilis RM125 hygromycin resistance (HmR, 100 μg/mL), spectinomycin resistance (SpR, 100 μg/mL), blasticidin S resistance (BSR, 500 μg/mL) 80650 MBS1005 BESN1009 Subgenome(356 kbp) leuB8 arg-15 hsdRM xkdE::pLS20coreH(HmR) neo Subgenome(356 kbp) Bacillus subtilis RM125 neomycin resistance (NmR, 15 μg/mL), hygromycin resistance (HmR, 100 μg/mL), spectinomycin resistance (SpR, 100 μg/mL), blasticidin S resistance (BSR, 500 μg/mL) The subgenome (356 kbp) is formed by pycA::eo-bsr yjcI::ne-spc. 80650 MBS1006 BESN1010 Subgenome(356 kbp) trpC2 recA362::tet Subgenome(356 kbp, HmR, bsr, spc) Bacillus subtilis 168 tetracycline resistance (TcR, 15 μg/mL), hygromycin resistance (HmR, 100 μg/mL), spectinomycin resistance (SpR, 100 μg/mL), blasticidin S resistance (BSR, 500 μg/mL) 80650 MBS1007 BESN1021 Subgenome(857 kbp) leuB8 arg-15 hsdRM xkdE::pLS20coreH(HmR) pycA::eo-bsr and yeeI-ne-spc-yeeK Bacillus subtilis RM125 neomycin resistance (NmR, 15 μg/mL), hygromycin resistance (HmR, 100 μg/mL), spectinomycin resistance (SpR, 100 μg/mL), blasticidin S resistance (BSR, 500 μg/mL) The subgenome (857 kbp) is formed by pycA::eo-bsr and yeeI-ne-spc-yeeK. 80650 MBS1008 BESN1024 Subgenome(857 kbp) trpC2 recA362::tet Subgenome(857 kbp, HmR, bsr, spc) Bacillus subtilis 168 tetracycline resistance (TcR, 15 μg/mL), hygromycin resistance (HmR, 100 μg/mL), spectinomycin resistance (SpR, 100 μg/mL), blasticidin S resistance (BSR, 500 μg/mL) 80650 MBS1009 ORe283 pUB307IPcoreH leuB8 arg-15 hsdRM pUB307IPcoreH Bacillus subtilis RM125 chloramphenicol resistance (5 μg/mL), tetracycline resistance (TcR, 15 μg/mL) 80650