MBS (Other Mutants) - Detail

Strain No MBS4
Strain Name RIK542
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers trpC2 delta-rrnHG1 delta-rrnO1 delta-rrnD1 delta-rrnE1 delta-rrnB2 delta-rrnA1 delta-rrnW2 delta-rrnJ1 :: cat
Selection Chloramphenicol resistance gene (cat); pC194 derivative
Lastest Markers Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat)
Parent (Donor)
Parent (Recipient) Bacillus subtilis 168
Method The detailed procedures for the construction of the strain are described in the paper entitled "Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growthand sporulation (Microbiology. [2010] 156 : 2944 - 2952)"
Source
Culture Condition L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on.
Other Remarks
Reference Nanamiya H, Sato M, Masuda K, Sato M, Wada T, Suzuki S, Natori Y, Katano M, Akanuma G, Kawamura F.
Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation.
Microbiology (Reading)  (2010)  156(Pt 10)   2944-2952  
[PubMed ID = 20634236]  [RRC reference
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