MBS (Other Mutants) - Detail

Strain No MBS34
Strain Name RIK1375
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers trpC2 delta-yjbM ywaC::cat relA::erm aprE::Pspac-ywaC D87G spc
Selection
Lastest Markers
Parent (Donor)
Parent (Recipient) Bacillus subtilis 168
Method The detailed procedures for the construction of the strain are described in the paper entitled "Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. (Microbiologyopen, [2012] 1(2): 115-134)"
Source
Culture Condition L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on.
Other Remarks
Reference Tagami K, Nanamiya H, Kazo Y, Maehashi M, Suzuki S, Namba E, Hoshiya M, Hanai R, Tozawa Y, Morimoto T, Ogasawara N, Kageyama Y, Ara K, Ozaki K, Yoshida M, Kuroiwa H, Kuroiwa T, Ohashi Y, Kawamura F.
Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp(0) mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome.
Microbiologyopen  (2012)  1(2)   115-34  
[PubMed ID = 22950019]  [RRC reference
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