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MBS (Other Mutants) - Detail

Strain No MBS21
Strain Name RIK827
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers trpC2 rplV::cat
Selection Chloramphenicol resistance gene (cat); pC194 derivative
Lastest Markers Tryptophan auxotroph (trpC2) and chloramphenicol resistance (cat)
Parent (Donor)
Parent (Recipient) Bacillus subtilis 168
Method The detailed procedures for the construction of the strain are described in the paper entitled "Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. (Journal of Bacteriology. [2012] 194(22): 6282-6291)"
Source
Culture Condition L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on.
Other Remarks
Reference Akanuma G, Nanamiya H, Natori Y, Yano K, Suzuki S, Omata S, Ishizuka M, Sekine Y, Kawamura F.
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol  (2012)  194(22)   6282-91  
[PubMed ID = 23002217]  [RRC reference
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