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MBS (Other Mutants) - Detail

Strain No MBS13
Strain Name RIK1001
Sex
Plasmid Integrated
Plasmid Autonoumous
Prophage
Chromosomal Markers trpC2 delta-yjbM relA :: erm
Selection
Lastest Markers
Parent (Donor)
Parent (Recipient) Bacillus subtilis 168
Method The detailed procedures for the construction of the strain are described in the paper entitled "Identification and functional analysis of novel [p]ppGpp synthetase genes in Bacillus subtilis (Molecular Microbiology, [2008] 67 : 291 - 304)"
Source
Culture Condition L-broth, Difco sporulation medium (DSM), two-strength Schaeffer's sporulation medium supplemented with glucose (2xSG), and so on.
Other Remarks
Reference Nanamiya H, Kasai K, Nozawa A, Yun CS, Narisawa T, Murakami K, Natori Y, Kawamura F, Tozawa Y.
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol  (2008)  67(2)   291-304  
[PubMed ID = 18067544]  [RRC reference
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