Inspection	PCR Product
Strain No		MGNA-A006
Constructor		DEVINE
Genotype		spoIISA
Original Name		BFS1801
BG		BG12677
Gene (Subtiwiki)		spoIISA
Locus Tag		BSU12830
Product		two-component apoptosis factor
Product (Old Name)		lethal when synthesized during vegetative growth in the absence of SpoIISB
BSORF		spoIISA
Length		248
Mutant		BFS1801
Forward Length		20
Forward Primer		ACAACCCACGTCATTTCTTC
Reverse Length		20
Reverse Primer		GATTCTTGCTGTATGCGGAC
The First Inspection	Length-1 (bp)	900
The First Inspection	Length-2 (bp)	300
The Second Inspection	Length-3 (bp)
Other Remarks
Reference		Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, Boland F, Brignell SC, Bron S, Bunai K, Chapuis J, Christiansen LC, Danchin A, Débarbouille M, Dervyn E, Deuerling E, Devine K, Devine SK, Dreesen O, Errington J, Fillinger S, Foster SJ, Fujita Y, Galizzi A, Gardan R, Eschevins C, Fukushima T, Haga K, Harwood CR, Hecker M, Hosoya D, Hullo MF, Kakeshita H, Karamata D, Kasahara Y, Kawamura F, Koga K, Koski P, Kuwana R, Imamura D, Ishimaru M, Ishikawa S, Ishio I, Le Coq D, Masson A, Mauël C, Meima R, Mellado RP, Moir A, Moriya S, Nagakawa E, Nanamiya H, Nakai S, Nygaard P, Ogura M, Ohanan T, O'Reilly M, O'Rourke M, Pragai Z, Pooley HM, Rapoport G, Rawlins JP, Rivas LA, Rivolta C, Sadaie A, Sadaie Y, Sarvas M, Sato T, Saxild HH, Scanlan E, Schumann W, Seegers JF, Sekiguchi J, Sekowska A, Séror SJ, Simon M, Stragier P, Studer R, Takamatsu H, Tanaka T, Takeuchi M, Thomaides HB, Vagner V, van Dijl JM, Watabe K, Wipat A, Yamamoto H, Yamamoto M, Yamamoto Y, Yamane K, Yata K, Yoshida K, Yoshikawa H, Zuber U, Ogasawara N. Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A (2003) 100(8) 4678-83 [PubMed ID = 12682299] [RRC reference]
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Gene Disrupted Strains - Detail