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The services on our website will be stopped during the period of time stated below due to the network maintenance work of NIG. We apologize for the inconvenience and we thank you for your understanding in this matter. Sat. 6th Jun. 2020 8:30 -17:30

Notice: Suspension of Services Due to the Network maintenance work of NIG

The services on our website will be stopped during the period of time stated below due to the network maintenance work of NIG. We apologize for the inconvenience and we thank you for your understanding in this matter.

Sat. 6th Jun. 2020 8:30 -17:30

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Gene Disrupted Strains - List

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Gene Disrupted Strains

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Strain No	Genotype	Original Name	Gene (Subtiwiki)	Locus Tag	Product	Product (Old Name)	Request
MGNA-A001	trpC2	168
MGNA-A002	spmA	BFS5	spmA	BSU23180	spore maturation protein	spore maturation protein
MGNA-A003	spmB	BFS6	spmB	BSU23170	spore maturation protein	spore maturation protein
MGNA-A004	ytbD	BFS34	ytbD	BSU29040	putative transporter	similar to antibiotic resistance protein
MGNA-A005	yteI	BFS23	sppA	BSU29530	signal peptide peptidase	signal peptide peptidase
MGNA-A006	spoIISA	BFS1801	spoIISA	BSU12830	two-component apoptosis factor	lethal when synthesized during vegetative growth in the absence of SpoIISB
MGNA-A007	yrrO	BFS2069	yrrO	BSU27340	putative hydrolase	similar to protease
MGNA-A008	yrrN	BFS2070	yrrN	BSU27350	putative hydrolase	similar to protease
MGNA-A009	yrbC	BFS2029	yrbC	BSU27820	conserved hypothetical protein	conserved protein
MGNA-A010	yrbB	BFS2028	coxA	BSU27830	spore cortex protein	spore cortex protein
MGNA-A011	yrbA	BFS2027	safA	BSU27840	morphogenetic protein associated with SpoVID	SpoVID associated factor A
MGNA-A012	ytgP	BFS2022	ytgP	BSU30050	Lipid II flippase having an ability to complement E. coli mviN , mutants lacking YtgP produce long cells and chains of cells	possible polysaccharide transporter, similar to spoVB
MGNA-A013	rapH	BFS2307	rapH	BSU06830	response regulator aspartate phosphatase	response regulator aspartate phosphatase
MGNA-A014	lytT	BFS2432	lytT	BSU28920	two-component response regulator	two-component response regulator. potential cognate sensor kinase is LytS
MGNA-A015	lytS	BFS2431	lytS	BSU28930	two-component sensor histidine kinase	two-component sensor histidine kinase. potential cognate response regulator is LytT
MGNA-A016	pksC	BFS2663	pksC	BSU17100	malonyl-CoA-acyltransferase involved inpolyketide synthesis	involved in polyketide synthesis
MGNA-A017	pksG	BFS2609	pksG	BSU17150	acetyl-S-AcpK beta-ketothioester polyketideintermediate transferase	putative polyketide biosynthesis protein pksG
MGNA-A018	pksR	BFS2610	pksR	BSU17220	polyketide synthase	polyketide synthase
MGNA-A019	aprX	BFS2601	aprX	BSU17260	alkaline serine protease	alkaline serine protease	Not available
MGNA-A020	ynbA	BFS2651	ynbA	BSU17430	putative GTP-binding protein protease modulator	similar to GTP-binding protein protease modulator
MGNA-A021	ynzH	BFS2669	ynzH	BSU17670		spore coat protein	Not available
MGNA-A022	yndD	BFS2671	yndD	BSU17750	putative spore germination protein	similar to spore germination protein
MGNA-A023	yndE	BFS2677	yndE	BSU17760	putative spore germination integral inner membrane protein	similar to spore germination protein
MGNA-A024	yndF	BFS2678	yndF	BSU17770	putative spore germination lipoprotein	similar to spore germination protein
MGNA-A025	ymfH	BFS2805	ymfH	BSU16860	putative processing protease	similar to processing protease
MGNA-A026	yvtB	BFS3042		BSU33000			Not available
MGNA-A027	yubE	BFS248	yubE	BSU31120		major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth
MGNA-A028	yraF	BFS432	yraF	BSU26960	putative spore coat protein	similar to spore coat protein
MGNA-A029	ytkC	BFS626	ytkC	BSU30640	putative autolytic amidase	conserved membrane protein
MGNA-A030	ytaA	BFS602	ytaA	BSU30920		spore coat protein
MGNA-A031	yjoB	BFS838	yjoB	BSU12420	ATPase possibly involved in protein degradation	similar to FtsH
MGNA-A032	yvoB	BFS871	hprK	BSU35000	serine/threonine protein kinase/phosphorylase	HPr(ser) kinase/phosphatase	Not available
MGNA-A033	yvgV	BFS1074	bdbD	BSU33480	thiol-disulfide oxidoreductase	thiol-disulfide oxidoreductases
MGNA-A034	ywqC	BFS1325	ywqC	BSU36260	modulator of YwqD protein tyrosine kinase activity	Modulator of YwqD tyrosine kinase
MGNA-A035	rapF	BFS1211	rapF	BSU37460	response regulator aspartate phosphatase	response regulator aspartate phosphatase
MGNA-A036	yukM	BFS1428					Not available
MGNA-A037	yfkR	YFKRd	yfkR	BSU07780	putative spore germination protein	similar to spore germination protein
MGNA-A038	yfkT	YFKTd	yfkT	BSU07760	putative spore germination integral inner membrane protein	similar to spore germination protein
MGNA-A039	yfkQ	YFKQd	yfkQ	BSU07790	putative spore germination protein	similar to spore germination protein	Not available
MGNA-A040	sspE	SSPEd	sspE	BSU08660	small acid-soluble spore protein (gamma-typeSASP)	small acid-soluble spore protein (major gamma-type SASP)
MGNA-A041	yqjG	YQJGd	yqjG	BSU23890		translocase for membrane proteins
MGNA-A042	pbpA	PBPA	pbpA	BSU25000	transpeptidase (penicillin-binding protein 2A)	penicillin-binding protein 2A
MGNA-A043	sipW	SIPWd	sipW	BSU24630	type I signal peptidase	type I signal peptidase
MGNA-A044	tasA	COTNd	tasA	BSU24620	major biofilm matrix component	translocation-dependent spore component
MGNA-A045	yqgT	YQGTd	yqgT	BSU24830	putative gamma-D-glutamyl-L-diamino acid endopeptidase	probable gamma-D-glutamyl-L-diamino acid endopeptidase I, peptidoglycan hydrolase
MGNA-A046	ccpB	YYAGd(CCPBd)	ccpB	BSU40870	transcriptional regulator involved in catabolite repression (LacI family)	transcriptional regulator involved in catabolite repression (LacI family)
MGNA-A047	yyxA	YYXAd	yyxA	BSU40360		similar to serine protease Do
MGNA-A048	phrG	PHRId	phrG	BSU40310	secreted regulator of the activity ofphosphatase RapG	regulator of the activity of phosphatase RapG
MGNA-A049	rapG	RAPGd	rapG	BSU40300	response regulator aspartate phosphatase	response regulator aspartate phosphatase
MGNA-A050	yaaT	YAATd	yaaT	BSU00320	involves in the transduction of signals to the phosphorelay for initiation of sporulation, similar to signal peptidase II	involves in the transduction of signals to the phosphorelay for initiation of sporulation, similar to signal peptidase II

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Gene Disrupted Strains - List