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BKE Genome-Scale deletion library (Gene-replacement by antibiotics resistance gene) - List

About BKE Genome-Scale deletion library
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We can distribute the strains which has buttons. The others will be ready when the strains are ready for distribute.
If you request all available gene disrupted strains, please push the order all button.

Order All	Request
BKE Genome-Scale deletion library (Gene-replacement by antibiotics resistance gene)	Agar plates, shipping at normal-temperature
	Glycerol stocks, shipping with dry ice

-Notes-
Although enough amount of dry ice will be included with the glycerol stocks,
the quality of the stocks and sublimation of dry ice due to transport delay cannot be guaranteed.
The glycerol stocks might be difficult to be delivered while dry ice is remaining depending on your country.
Please contact us before ordering.

We can distribute BKE library solely to researchers at academic and non-profit organizations for non-commercial and research purposes.
Please contact NIG INNOVATION in National Institute of Genetics (chizai [at] nig.ac.jp) if you belong to profit organization.

We don’t distribute the parental strain which was used to construct BKE library.
Please purchase one from BGSC (Bacillus Genetic Stock Center; http://bgsc.org), US.
The strain No. is BGSC No. 1A1.

How to find the strain information
All List | Request Available Strains

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Strain No	Genotype	Original Name	Gene (Subtiwiki)	Locus Tag	Product	Request
BKE00100	dacA	BKE00100	dacA	BSU00100	D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5)
BKE38640	yxlH	BKE38640	yxlH	BSU38640	putative efflux transporter
BKE01660	ybbD	BKE01660	ybbD	BSU01660	putative beta-hexosaminidase, putative lipoprotein
BKE01960	skfF	BKE01960	skfF	BSU01960	sporulation killing factor biosynthesis and export; ABC transporter (permease)
BKE02640	tatCD	BKE02640	tatCD	BSU02640	component of the twin-arginine pre-protein translocation pathway
BKE02760	natB	BKE02760	natB	BSU09900	Na+ ABC efflux transporter (permease)
BKE02980	opuAA	BKE02980	opuAA	BSU02980	glycine betaine ABC transporter (ATP-bindingprotein)
BKE03380	yckB	BKE03380	yckB	BSU03380	putative ABC transporter (binding lipoprotein)
BKE03810	yclO	BKE03810	yclO	BSU03810	transporter for petrobactin (PB), a photoreactive 3,4-catecholate siderophore, permease protein, Fur regulon
BKE04480	ydbI	BKE04480	ydbI	BSU04480	putative integral inner membrane protein
BKE05420	ydfI	BKE05420	ydfI	BSU05420	two-component response regulator
BKE06400	yebE	BKE06400	yebE	BSU06400	conserved hypothetical protein
BKE00490	spoVG	BKE00490	spoVG	BSU00490	regulator required for spore cortex synthesis (stage V sporulation)
BKE01460	ybaE	BKE01460	ybaE	BSU01460	ABC efflux transporter (ATP-binding protein)
BKE01670	ybbE	BKE01670	ybbE	BSU01670	putative protein precursor; similar to beta-lactamase
BKE02010	ybdK	BKE02010	ybdK	BSU02010	two-component sensor histidine kinase
BKE02670	lmrB	BKE02670	lmrB	BSU02670	drug-export protein, lincomycin-resistance protein
BKE02780	ycdA	BKE02780	ycdA	BSU02780	putative lipoprotein
BKE02990	opuAB	BKE02990	opuAB	BSU02990	glycine betaine ABC transporter (permease)
BKE03520	srfAD	BKE03520	srfAD	BSU03520	surfactin synthetase
BKE03820	yclP	BKE03820	yclP	BSU03820	transporter for petrobactin (PB), a photoreactive 3,4-catecholate siderophore, ATP-binding protein, Fur regulon
BKE04490	ydbJ	BKE04490	ydbJ	BSU04490	putative ABC transporter (ATP-binding protein)
BKE05430	ydfJ	BKE05430	ydfJ	BSU05430	putative proton metabolite efflux transporter
BKE06570	yerB	BKE06570	yerB	BSU06570	putative lipoprotein
BKE38540	dltE	BKE38540	dltE	BSU38540	putative oxidoreductase
BKE01530	cwlD	BKE01530	cwlD	BSU01530	N-acetylmuramoyl-L-alanine amidase, peptidoglycan hydrolase
BKE01700	murQ	BKE01700	murQ	BSU01700	D-lactyl ether N-acetylmuramic-6-phosphate acidetherase
BKE02250	ybfJ	BKE02250	ybfJ	BSU02250	putative lipoprotein
BKE02690	ansZ	BKE02690	ansZ	BSU02690	L-asparaginase (putative lipoprotein), activated during nitrogen-limited growth by TnrA
BKE02810	cwlK	BKE02810	cwlK	BSU02810	murein L,D:-endopeptidase
BKE03000	opuAC	BKE03000	opuAC	BSU03000	glycine betaine ABC transporter (glycinebetaine-binding lipoprotein)
BKE03530	ycxA	BKE03530	ycxA	BSU03530	putative permease
BKE03830	yclQ	BKE03830	yclQ	BSU03830	transporter for petrobactin (PB), a photoreactive 3,4-catecholate siderophore, substrate-binding protein, Fur regulon
BKE04540	ydbO	BKE04540	ydbO	BSU04540	putative cation efflux system
BKE05470	ydfM	BKE05470	ydfM	BSU05470	putative divalent cation efflux transporter
BKE06630	yerH	BKE06630	yerH	BSU06630	putative lipoprotein
BKE00560	spoVT	BKE00560	spoVT	BSU00560	transcriptional regulator of sigma-G-dependent genes
BKE01550	gerD	BKE01550	gerD	BSU01550	lipoprotein with a role in spores' rapid response to nutrient germinants
BKE01740	rsiW	BKE01740	rsiW	BSU01740	anti-sigma(W) factor
BKE02480	gudP	BKE02480	gudP	BSU02480	glucarate transporter, D-glucarate/galactarate utilization
BKE02700	estA	BKE02700	estA	BSU02700	secreted alkaliphilic lipase
BKE02850	adcA	BKE02850	adcA	BSU02850	Zn(II)-binding lipoprotein, Zur regulon
BKE03020	ycgA	BKE03020	ycgA	BSU03020	putative integral inner membrane protein
BKE03590	tcyC	BKE03590	tcyC	BSU03590	low-affinity cystine ABC transporter (ATP-binding protein)
BKE03920	glcU	BKE03920	glcU	BSU03920	glucose permease
BKE38690	yxlC	BKE38690	yxlC	BSU38690	sigma-Y antisigma factor	Not available
BKE05680	ydgK	BKE05680	ydgK	BSU05680	putative efflux transporter
BKE06660	opuE	BKE06660	opuE	BSU06660	proline transporter
BKE00570	yabM	BKE00570	yabM	BSU00570	possible polysaccharide transporter, similar to Lipid II flippase, mutants lacking YabM exhibit sensitivity to moenomycin
BKE01580	ybaR	BKE01580	ybaR	BSU01580	putative permease

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BKE Genome-Scale deletion library (Gene-replacement by antibiotics resistance gene) - List