breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 165,749 Δ1 bp intergenic (+42/‑5) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn
RA 165,751 Δ1 bp intergenic (+44/‑3) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn
RA 165,826 +C intergenic (+1/‑4) trnI‑Asn → / → trnI‑Thr tRNA‑Asn/tRNA‑Thr
RA 166,037 +T intergenic (+4/‑27) trnI‑Gly → / → trnI‑Arg tRNA‑Gly/tRNA‑Arg
RA 166,345 Δ1 bp intergenic (+17/‑155) trnI‑Ala → / → rrnH‑16S tRNA‑Ala/ribosomal RNA‑16S
RA 490,812 G→T P78T (CCT→ACT)  ydzK ← hypothetical protein
RA 557,870 (T)5→6 intergenic (+421/‑3) yddT → / → ydzN putative exported phage protein/hypothetical protein
RA 608,219 (A)5→6 intergenic (‑144/+27) ydgF ← / ← dinB putative amino acid permease/nuclease inhibitor
RA 756,382 C→A P82T (CCA→ACA)  cotJB → component of the inner spore coat
RA 1,317,152 +GT intergenic (+23/+5) phrA → / ← yjpA secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
RA 1,317,158 (T)5→6 coding (257/258 nt) yjpA ← putative enzyme
RA 1,769,727 T→C F263L (TTT→CTT)  pdeB → 2'3' and 3'5' cyclic nucleotide monophosphates phosphodiesterase involved in biofilm formation
RA 1,769,799 T→C intergenic (+64/‑136) pdeB → / → spoVS 2'3' and 3'5' cyclic nucleotide monophosphates phosphodiesterase involved in biofilm formation/regulator required for dehydratation of the spore core and assembly of the coat (stage V sporulation)
RA 1,769,812 A→G intergenic (+77/‑123) pdeB → / → spoVS 2'3' and 3'5' cyclic nucleotide monophosphates phosphodiesterase involved in biofilm formation/regulator required for dehydratation of the spore core and assembly of the coat (stage V sporulation)
RA 2,097,087 (A)7→8 intergenic (‑111/+39) azoJ ← / ← yocK FMN‑dependent NADH‑azoreductase/putative general stress protein
RA 2,271,424 T→C R78R (AGA→AGG uvrX ← lesion bypass phage DNA polymerase; phage SPbeta
RA 2,271,505 C→T V51V (GTG→GTA uvrX ← lesion bypass phage DNA polymerase; phage SPbeta
RA 2,271,523 A→C D45E (GAT→GAG uvrX ← lesion bypass phage DNA polymerase; phage SPbeta
RA 2,480,646 2 bp→AT intergenic (+21/+103) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,480,654 Δ1 bp intergenic (+29/+96) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,480,667 Δ1 bp intergenic (+42/+83) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,581,732 (T)6→7 intergenic (‑115/+39) pstS ← / ← pbpA phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)
RA 3,770,066 (A)8→9 intergenic (‑613/+38) ywzE ← / ← csbD conserved protein of unknown function (putative phage interference protein)/stress response protein
RA 3,935,825 Δ1 bp coding (1190/1191 nt) rlmI ← large subunit ribosomal RNA m5C1962 methyltransferase
RA 3,948,008 T→A L284H (CTT→CAT)  tyrZ → tyrosyl‑tRNA synthetase
RA 4,155,395 (A)5→6 intergenic (‑186/+38) trnY‑Lys ← / ← purA tRNA‑Lys/adenylosuccinate synthetase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000964 161181–161238 161505–161245 8–325 8 [7] [7] 8 rrnI‑16S ribosomal RNA‑16S
* * ÷ NC_000964 161512–164866 165465–164903 38–3954 8 [7] [5] 8 [rrnI‑16S]–[rrnI‑23S] [rrnI‑16S],[rrnI‑23S]
* * ÷ NC_000964 166715–166766 171084–170392 3627–4370 8 [7] [7] 8 [rrnH‑16S]–[rrnH‑23S] [rrnH‑16S],[rrnH‑23S]
* * ÷ NC_000964 1769908 1868374 98467 8 [7] [0] 30 spoVS–[ymzA] spoVS,tdh,kbl,miaB,ricA,cotE,mutS,mutL,ymzD,ymcC,pksA,pksB,BSU_17099,pksC,pksD,pksE,acpK,pksF,pksG,pksH,pksI,pksJ,pksL,pksM,pksN,pksR,pksS,ymzB,ymaE,aprX,pghC,ymaD,ebrB,ebrA,ymaG,ymaF,miaA,hfq,ymzC,[ymzA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000964 1868375 =0 (0.000)25 (0.590)
+36 bp
24/412 0.8 59.5% intergenic (+1/‑242) ymzA/nrdI hypothetical protein/co‑factor of ribonucleotide diphosphate reductase
?NC_000964 3788113 = 40 (0.800)intergenic (‑110/+313) atpI/upp ATP synthase (subunit i)/uracil phosphoribosyltransferase