breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 165,749 Δ1 bp intergenic (+42/‑5) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn
RA 165,751 Δ1 bp intergenic (+44/‑3) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn
RA 165,826 +C intergenic (+1/‑4) trnI‑Asn → / → trnI‑Thr tRNA‑Asn/tRNA‑Thr
RA 166,037 +T intergenic (+4/‑27) trnI‑Gly → / → trnI‑Arg tRNA‑Gly/tRNA‑Arg
RA 166,345 Δ1 bp intergenic (+17/‑155) trnI‑Ala → / → rrnH‑16S tRNA‑Ala/ribosomal RNA‑16S
RA 490,812 G→T P78T (CCT→ACT)  ydzK ← hypothetical protein
RA 557,870 (T)5→6 intergenic (+421/‑3) yddT → / → ydzN putative exported phage protein/hypothetical protein
RA 608,219 (A)5→6 intergenic (‑144/+27) ydgF ← / ← dinB putative amino acid permease/nuclease inhibitor
RA 756,382 C→A P82T (CCA→ACA)  cotJB → component of the inner spore coat
RA 1,176,825 A→G L143L (CTT→CTC yitG ← putative efflux transporter
RA 1,176,945 A→G Y103Y (TAT→TAC yitG ← putative efflux transporter
RA 1,177,136 G→A L40F (CTT→TTT)  yitG ← putative efflux transporter
RA 1,194,908 (T)8→9 intergenic (+81/‑126) yitZ → / → argC putative transport protein/N‑acetylglutamate gamma‑semialdehyde dehydrogenase
RA 1,195,173 A→G H47R (CAT→CGT)  argC → N‑acetylglutamate gamma‑semialdehyde dehydrogenase
RA 1,195,446 T→C I138T (ATT→ACT)  argC → N‑acetylglutamate gamma‑semialdehyde dehydrogenase
RA 1,317,152 +GT intergenic (+23/+5) phrA → / ← yjpA secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
RA 1,317,158 (T)5→6 coding (257/258 nt) yjpA ← putative enzyme
RA 2,097,087 (A)7→8 intergenic (‑111/+39) azoJ ← / ← yocK FMN‑dependent NADH‑azoreductase/putative general stress protein
RA 2,271,424 T→C R78R (AGA→AGG uvrX ← lesion bypass phage DNA polymerase; phage SPbeta
RA 2,271,505 C→T V51V (GTG→GTA uvrX ← lesion bypass phage DNA polymerase; phage SPbeta
RA 2,271,523 A→C D45E (GAT→GAG uvrX ← lesion bypass phage DNA polymerase; phage SPbeta
RA 2,480,646 2 bp→AT intergenic (+21/+103) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,480,654 Δ1 bp intergenic (+29/+96) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,480,667 Δ1 bp intergenic (+42/+83) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,581,732 (T)6→7 intergenic (‑115/+39) pstS ← / ← pbpA phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)
RA 3,770,066 (A)8→9 intergenic (‑613/+38) ywzE ← / ← csbD conserved protein of unknown function (putative phage interference protein)/stress response protein
RA 3,935,825 Δ1 bp coding (1190/1191 nt) rlmI ← large subunit ribosomal RNA m5C1962 methyltransferase
RA 3,948,008 T→A L284H (CTT→CAT)  tyrZ → tyrosyl‑tRNA synthetase
RA 4,155,395 (A)5→6 intergenic (‑186/+38) trnY‑Lys ← / ← purA tRNA‑Lys/adenylosuccinate synthetase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000964 161166–161240 161471 232–306 4 [3] [3] 4 rrnI‑16S ribosomal RNA‑16S
* * ÷ NC_000964 161535–164898 165474–164903 6–3940 4 [3] [3] 6 [rrnI‑16S]–[rrnI‑23S] [rrnI‑16S],[rrnI‑23S]
* * ÷ NC_000964 166747 171073–170393 3647–4327 4 [3] [3] 4 [rrnH‑16S]–[rrnH‑23S] [rrnH‑16S],[rrnH‑23S]
* * ÷ NC_000964 171577–171679 176091–172533 855–4515 4 [3] [3] 5 [rrnG‑16S]–[rrnG‑23S] [rrnG‑16S],[rrnG‑23S]
* * ÷ NC_000964 1177359 1194824 17466 4 [3] [0] 21 yitH–[yitZ] yitH,yitI,samT,yitK,yitL,yitM,yitO,yitP,yizB,yitQ,yitR,nprB,fakBB,yitT,ipi,yizC,ribZC,yitV,yitW,yitY,[yitZ]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000964 = 115370210 (0.260)5 (0.140) 5/478 2.8 34.8% intergenic (+81/‑87) yisL/wprA putative integral membrane protein/cell wall‑associated protease
?NC_000964 1153714 = 9 (0.240)intergenic (+93/‑75) yisL/wprA putative integral membrane protein/cell wall‑associated protease
* ? NC_000964 1194825 =0 (0.000)18 (0.550)
+36 bp
17/420 0.8 61.9% coding (493/495 nt) yitZ putative transport protein
?NC_000964 3788113 = 26 (0.680)intergenic (‑110/+313) atpI/upp ATP synthase (subunit i)/uracil phosphoribosyltransferase