Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	2,480,646	2 bp→AT	intergenic (+21/+103)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	2,480,646	0	T	A	100.0%	93.1 / NA	26	intergenic (+21/+104)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/11);  total (15/11)
*	NC_000964	2,480,647	0	A	T	100.0%	96.2 / NA	26	intergenic (+22/+103)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/11);  total (15/11)

CCCTGAAGCATATGAAAAACTAATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCTT  >  NC_000964/2480403‑2480891                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                     cccTGAAGCATATGAAAAACTAATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAATACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGAGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAc                                                                                                                                                                                                                                                >  2:158996/1‑251 (MQ=255)     gAAGCATATGAAAAACTAATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAcaagc                                                                                                                                                                                                                                            <  1:45627/251‑5 (MQ=255)            atGAAAAACTAATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTc                                                                                                                                                                                                                                     <  1:42939/250‑1 (MQ=255)                      aaTTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTa                                                                                                                                                                                                                          <  1:290223/250‑1 (MQ=255)                          cACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTg                                                                                                                                                                                                                     >  2:105595/1‑251 (MQ=255)                            cGACTGTCTTCTTGGCGATGCAACCAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGa                                                                                                                                                                                                                    >  1:78821/1‑250 (MQ=255)                                           cGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                                                                                                                    >  2:20434/1‑251 (MQ=255)                                            gATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTAGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                                                                                                                   >  1:185081/1‑251 (MQ=255)                                                     aaCTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaata                                                                                                                                                                                          <  2:268378/251‑1 (MQ=255)                                                        tttGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAAc                                                                                                                                                                                        >  1:8131/1‑250 (MQ=255)                                                                            tGCCCTTTCTTTGAGCTTTGTCGTCTCGATTTCTGAAACCTGGGCAGCAAACAAAACCCTAACCCCTAACCACGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTTGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTTC‑AAGCATTTTCGG‑CTTTTTTACGCCGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAaga                                                                                                                                                                    <  1:262834/250‑1 (MQ=255)                                                                               ccTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                                                                                                                          <  1:147802/209‑1 (MQ=255)                                                                               ccTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                                                                                                                          >  2:147802/1‑209 (MQ=255)                                                                                       ggAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGtt                                                                                                                                                        >  1:151752/1‑251 (MQ=255)                                                                                                        acttCTGAAACAAGGGCAACAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATTGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCCTACACTGGGGGAACATATAATAAGAAAAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAATAACTTCGGGGGGTTCTATTGATTACTTc                                                                                                                                         <  1:247417/247‑1 (MQ=255)                                                                                                              gAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                                                                                                                  <  1:343289/250‑1 (MQ=255)                                                                                                                                  aaCCTTATCTCCTAACTACGAAGCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGGTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCcttctt                                                                                                             >  1:38283/1‑251 (MQ=255)                                                                                                                                          ctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGt                                                                                                      >  1:266245/1‑250 (MQ=255)                                                                                                                                             cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGc                                                                                                  >  2:202170/1‑251 (MQ=255)                                                                                                                                                aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTc                                                                                                >  1:218225/1‑250 (MQ=255)                                                                                                                                                                 aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCaaa                                                                              <  1:100556/251‑1 (MQ=255)                                                                                                                                                                    ggACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAagt                                                                            >  1:276480/1‑250 (MQ=255)                                                                                                                                                                                                            tACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGc                                    <  1:183265/250‑1 (MQ=255)                                                                                                                                                                                                                   gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGt                            >  1:18925/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAAt        <  1:196420/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGAATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCtt  >  2:289019/1‑251 (MQ=255)                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                     CCCTGAAGCATATGAAAAACTAATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCTT  >  NC_000964/2480403‑2480891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑