Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	756,382	C→A	P82T (CCA→ACA)	cotJB →	component of the inner spore coat

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	756,382	0	C	A	100.0%	100.3 / NA	32	P82T (CCA→ACA)	cotJB	component of the inner spore coat
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/17);  total (15/17)

AAAGGTTGACGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGCCATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCATCGGCCTTTTAACAGATATCGGCACGGAGGAGTTTGCCCATTTGGAAATGAT  >  NC_000964/756147‑756628                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       aaaGGTTGACGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTTTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAg                                                                                                                                                                                                                                         <  2:109452/251‑1 (MQ=255)  aaGGTTGACGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGt                                                                                                                                                                                                                                        <  1:330073/251‑1 (MQ=255)   aGGTTGACGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGta                                                                                                                                                                                                                                       <  1:346443/251‑1 (MQ=255)   aGGTTGACGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGCGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCACTATTCCGGCTATTCAAGGCACTTAACAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCACGGATTGGGATTGGGGAAAAGGGACATGCCCGTGGCAAGt                                                                                                                                                                                                                                        >  1:104345/1‑250 (MQ=255)        gACGCCGAATATTAACGTAAGCTAGAGAAAATACAGGCGGCTGATTTTGAGCTTGTTGGGCTGAGACTTTATTTAAATACACATCCTCATGAGGAAGATGCGTTGAAGCAATTCAATCAATATTCAGGCTATTCAAGGCACTTAAAAAGCCAGTTAGAATCATCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGACGTGGCAAGTATAagg                                                                                                                                                                                                                                  >  2:63159/1‑251 (MQ=255)          cGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAaggag                                                                                                                                                                                                                                <  2:178659/251‑1 (MQ=255)                          catcTAGAGCAAATACAGGCTTCTGATTTTGTGCTGGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACGTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTACTGCAGTGCGGCACCCGCCCCGCGGGCAAGGGTGGGGACGGTGGAAAAGGGACATGGCCGTGGCAGGTATAAGGAGGAATGCCTGAAggtg                                                                                                                                                                                                                 <  2:298835/247‑1 (MQ=255)                          cAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCCAGTATAAGGCGGAATGCCTGAAtgggg                                                                                                                                                                                                                >  2:29848/1‑248 (MQ=255)                             cTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGAAGAGTCTTTCTTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGgtg                                                                                                                                                                                                             >  2:121162/1‑251 (MQ=255)                               agagCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGgtg                                                                                                                                                                                                             <  2:156553/249‑1 (MQ=255)                                gagCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTAt                                                                                                                                                                                                          <  1:261220/251‑1 (MQ=255)                                                  gATTTTGTGCTGGTTGAGATGAGTATTTATGTAAATACACAGCCTAATGATGAAGATGCGTGGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTGCGGACCGCTTCTGCAGTTCGGCAACAGGCCCGTGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGGAGAAGGAGGAATGACTGAATGTGGGGGTATGAAAAGAAGTTGCAATa                                                                                                                                                                                         <  2:291556/250‑1 (MQ=255)                                                         tgCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTc                                                                                                                                                                                  >  2:158221/1‑250 (MQ=255)                                                                     tGAGTCTTTCTTTAAATACACATCCTCATGATGCAGATGCGTTGACGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTACAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGCATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGt                                                                                                                                                                     >  2:261540/1‑251 (MQ=255)                                                                                    aTACACATCCTCAGGATGAAGATGCGGGGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCCCTTAAAAAGACATTTCGAATCCTCTTACTGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAGCCCGACGCTgg                                                                                                                                                      <  2:306264/251‑1 (MQ=255)                                                                                           tCCTCATGATGAAGATGCGTTGTAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGACTGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGt                                                                                                                                                >  2:282815/1‑250 (MQ=255)                                                                                                atgatgAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATttg                                                                                                                                           >  1:211225/1‑250 (MQ=255)                                                                                                     gaAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATttgattg                                                                                                                                       >  1:256650/1‑249 (MQ=255)                                                                                                      aaGATGCGATGAAGAAATTCAATCAATATTCCGGCTCTTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACAGCTTCTGCAGTTCGGCACCAGCCCTGCGGGCAAGGATTGGGATTGGGGAAATGGGACATGGCCGTGGCAAGTATAAGGAGGAAAGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATCCCCTGTCAAGGTCAGTACGCGCAACCCGACGCTGGCGAAGTATTTGATTGAGc                                                                                                                                    >  2:105237/1‑251 (MQ=255)                                                                                                                gAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGgcgg                                                                                                                          >  1:247388/1‑251 (MQ=255)                                                                                                                  aGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGgcgg                                                                                                                          >  2:137402/1‑249 (MQ=255)                                                                                                                                                           aGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAAcc                                                                                 >  1:163411/1‑249 (MQ=255)                                                                                                                                                                   cGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAGAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTtata                                                                        <  1:260534/250‑1 (MQ=255)                                                                                                                                                                   cGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATAc                                                                       >  1:83652/1‑251 (MQ=255)                                                                                                                                                                             tACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTg                                                               <  2:113507/249‑1 (MQ=255)                                                                                                                                                                              actgtgcGCTTCTGCAGGTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGTGGAATGCCTGAATGGGGGTGTATGAAAAGAAGCTGCAATACCCTGACAAGGTCAGTACGTGCAACCCTACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTTGCCGCGGCTATCCGGTATTTGAACCAGCGTTATACTATTGCTGAt                                                             <  2:104345/244‑1 (MQ=255)                                                                                                                                                                                  aCCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGt                                                        <  1:156727/251‑1 (MQ=255)                                                                                                                                                                                           gCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCATCGGCCt                                               >  2:43887/1‑251 (MQ=255)                                                                                                                                                                                                            ccGCGGGCAAGGATTGGGATTGGGGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCATCGGCCTTTTAACAGATATCGGCa                              <  1:333516/251‑1 (MQ=255)                                                                                                                                                                                                                  gCAAGGATTGGGATTGGGGAAAAGTGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGGAAAGAAGCTGCAAGACCCTGTCAAGGTCAGTACGTGCACCCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCTGACGGCGAGCGGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCACCGGCCTTTTAACAGATATCGGCACggagg                        <  2:122932/251‑1 (MQ=255)                                                                                                                                                                                                                                tgggGAAAAGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCATCGGCCTTTTAACAGATATCGGCACGGAGGAGTTTGCCCAttt           <  1:29848/250‑1 (MQ=255)                                                                                                                                                                                                                                        aGGGACATGGCCGTGGCAAGTATAAGGAGGAATGCCAGAATGTGGGTGTATGAAAAGAAGCTGCAATACCATGTCAAGGGCAGTACGTGCCACCCGAAGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCATCGGCCTTTTAACAGGTATCGGCACGGAGGAGTTTGCCCATTTGGAAATGAt  <  2:211225/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       AAAGGTTGACGCCGAATATTATCGTCAGCTAGAGCAAATACAGGCTGCTGATTTTGTGCTTGTTGAGCTGAGTCTTTATTTAAATACACATCCTCATGATGAAGATGCGTTGAAGCAATTCAATCAATATTCCGGCTATTCAAGGCACTTAAAAAGACAGTTCGAATCCTCTTACGGACCGCTTCTGCAGTTCGGCAACAGCCCCGCGGGCAAGGATTGGGATTGGGGAAAAGGGCCATGGCCGTGGCAAGTATAAGGAGGAATGCCTGAATGTGGGTGTATGAAAAGAAGCTGCAATACCCTGTCAAGGTCAGTACGTGCAACCCGACGCTGGCGAAGTATTTGATTGAGCAGTATGGCGGAGCGGACGGCGAGCTGGCCGCGGCTCTCCGGTATTTGAACCAGCGTTATACGATTCCTGATAAGGTCATCGGCCTTTTAACAGATATCGGCACGGAGGAGTTTGCCCATTTGGAAATGAT  >  NC_000964/756147‑756628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑