Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	3,948,008	T→A	L284H (CTT→CAT)	tyrZ →	tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	3,948,008	0	T	A	100.0%	174.9 / NA	46	L284H (CTT→CAT)	tyrZ	tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/24);  total (22/24)

AGGCGGAACGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCTTGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTAAAGCTCCTCT  >  NC_000964/3947763‑3948253                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aGGCGGAACGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGaa                                                                                                                                                                                                                                                   <  1:95400/250‑1 (MQ=255)    cGGAACGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAag                                                                                                                                                                                                                                                  >  2:179746/1‑248 (MQ=255)     ggAACGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGaa                                                                                                                                                                                                                                              <  2:319310/251‑1 (MQ=255)       aaCGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGCTGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGaaa                                                                                                                                                                                                                                             >  2:282395/1‑250 (MQ=255)        aCGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGACACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGaaaaa                                                                                                                                                                                                                                           <  1:341869/251‑1 (MQ=255)            aTCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAAc                                                                                                                                                                                                                                         >  1:222398/1‑249 (MQ=255)                 cATTTCAATGTCCTCATGGGACGGCATTTGCAGTAACGATACAACAAAGAAAACCAAGTCGTAATCCTTATGCCTCTCTTGGAAGGAGTGGATGGTGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGTTGTCCCTGCCCGACAGCCTTATGAAAAAGTACCTCCACTTGGCGACGGACTTAGAGCATGATGAGAAAAAACatcaagt                                                                                                                                                                                                                                  <  2:64999/251‑7 (MQ=255)                     tCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTaaa                                                                                                                                                                                                                               >  1:253605/1‑250 (MQ=255)                       aaTGTCCTCATGGGACAGCATTTCCAAGACCGATACCACAAAAAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGACGAGAAAATGTCGAAGACGAAACACAACTACATCGGCATTAACGAACATCCAAACGACATATACGGAAAAACGATGTAACTGCCCGACAGCCTGATGAAAAAGTACATACACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGaaaacg                                                                                                                                                                                                                             >  2:106145/1‑248 (MQ=255)                                aTGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTAAGAAAc                                                                                                                                                                                                                   >  2:233109/1‑251 (MQ=255)                                                         acaacaAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGcc                                                                                                                                                                                          <  2:179320/251‑1 (MQ=255)                                                         acaacaAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGc                                                                                                                                                                                           <  2:169111/250‑1 (MQ=255)                                                               aaGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATg                                                                                                                                                                                    <  1:251/251‑1 (MQ=255)                                                                    aaaCAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCtttt                                                                                                                                                                               <  1:3886/251‑1 (MQ=255)                                                                            cgtcATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGACAATGTCGAAGTCGAACCACAACTACATTGGCATTAACGAACCTCCAAACGACATTGACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTAAGAGCATGAGGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGACGCCGTTCATCCGCGTGATGCCAACATCGTTTTAGCCAGa                                                                                                                                                                        >  2:318696/1‑250 (MQ=255)                                                                                aTCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAAt                                                                                                                                                                   >  1:256649/1‑251 (MQ=255)                                                                                  ccTTATGCCTCTATTGGAAGGCTTGGATGGCTTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGAATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAACAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGACACCGGCGCCGATCATCCGCGTGATGCCAAAATGCTTTTAGCAAGAAAAAt                                                                                                                                                                   >  2:175545/1‑249 (MQ=255)                                                                                  ccTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCCGAACAATc                                                                                                                                                                  <  2:149176/250‑1 (MQ=255)                                                                                         ccGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAAt                                                                                                                                                          <  1:233109/251‑1 (MQ=255)                                                                                            ctctTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGt                                                                                                                                                        >  2:193181/1‑250 (MQ=255)                                                                                               ttGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATc                                                                                                                                                     >  1:325738/1‑250 (MQ=255)                                                                                                 ggAAGGCTTGGATGGCGTCGAGAAAATGACGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCAc                                                                                                                                                   <  1:140728/250‑1 (MQ=255)                                                                                                    aGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGgag                                                                                                                                               <  1:196383/251‑1 (MQ=255)                                                                                                     ggCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGgaga                                                                                                                                              <  1:258241/251‑1 (MQ=255)                                                                                                         tGGATTGCGTCGATAAAATGTCGAAGTCGAAAACCATCTACATTGGCATTAACGAACAGCCAAACGACATTGACGGAAAAACGATGTCACTGCCAGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCACAACAATCGTTCGAATGTATCACGgagaga                                                                                                                                            <  2:296882/249‑1 (MQ=255)                                                                                                          ggATCGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTAACTGCCCGACAGCCTGATGAAAAAGTACATCCCCTTGGCGAAAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGGTCATCCGCGTCATGCCAAAATGCTTTTAGCCAGAGCAATCGTCCGAATGTATCACGGAGAGaa                                                                                                                                           >  1:86597/1‑249 (MQ=255)                                                                                                                    gagaAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGc                                                                                                                               >  2:96792/1‑251 (MQ=255)                                                                                                                                    cgaaACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGttt                                                                                                               <  2:78638/251‑1 (MQ=255)                                                                                                                                    cgaaACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGttt                                                                                                               >  1:228043/1‑251 (MQ=255)                                                                                                                                    cgaaACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGtt                                                                                                                <  1:307994/250‑1 (MQ=255)                                                                                                                                           caACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAAc                                                                                                          >  1:53432/1‑249 (MQ=255)                                                                                                                                                       cATTAACGAACATACAAAAGACATGTACGGAAAAACGATGTAACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTACAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCATTTAGCAAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGATGCAGAACACTCATTTAAAACAgaatatcaggaa                                                                                             >  2:245867/1‑239 (MQ=255)                                                                                                                                                                                          cGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTgg                                                         <  1:36177/251‑1 (MQ=255)                                                                                                                                                                                           gATGTCACTGCCCGACAGCCTGATGAAAAAGAACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTgg                                                         >  1:250635/1‑250 (MQ=255)                                                                                                                                                                                              gTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCACCCGCGTGATGCCAAAATGCTTTTGGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAg                                                     <  2:222398/251‑1 (MQ=255)                                                                                                                                                                                                    gCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGaa                                                <  2:159536/250‑1 (MQ=255)                                                                                                                                                                                                          cAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACg                                          >  2:32758/1‑250 (MQ=255)                                                                                                                                                                                                                  tgaAAAAGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCgatg                                 >  1:327625/1‑251 (MQ=255)                                                                                                                                                                                                                     aaaaGTACATCCGCTTGGCGACAGACTTAGAGCAGGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAGCCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGAtgatt                              <  2:222302/251‑1 (MQ=255)                                                                                                                                                                                                                        aGTACATCCACTTGGCGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGAtgattgat                           <  2:147601/251‑1 (MQ=255)                                                                                                                                                                                                                            cATCCACTTGGCGTGAGTCTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATTCCAAAATGCTTTAAGCCAAAACAATCGTCCGAATGTATTACGGAGAGAAAACAGCAGAAGCTGCCGAACAATCGTTTAAAACAGTCTTACAGAAAAACAGCCTGCCTGAAGATATACCGACCGTAAACTGGAAATGCGAAAAAACGATAGCGATGACTGATCTGc                       <  2:229434/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTa            <  1:90804/251‑1 (MQ=255)                                                                                                                                                                                                                                        gACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTa            >  1:275583/1‑250 (MQ=255)                                                                                                                                                                                                                                        gACAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGAAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTaa           <  1:215108/251‑1 (MQ=255)                                                                                                                                                                                                                                         aCAGACTTAGAGCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTaa           >  2:30441/1‑250 (MQ=255)                                                                                                                                                                                                                                                 agagCATGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTAAAGCTCctct  >  1:307736/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AGGCGGAACGGATCAGCATTTCAATGTCCTCATGGGACGGCATTTCCAAGAACGATACAACAAAGAAAAACAAGTCGTCATCCTTATGCCGCTCTTGGAAGGCTTGGATGGCGTCGAGAAAATGTCGAAGTCGAAACACAACTACATTGGCATTAACGAACATCCAAACGACATGTACGGAAAAACGATGTCACTGCCCGACAGCCTGATGAAAAAGTACATCCACTTGGCGACAGACTTAGAGCTTGAAGAGAAAAAACAGCTCGTAAAAGACTTAGAAACCGGCGCCGTTCATCCGCGTGATGCCAAAATGCTTTTAGCCAGAACAATCGTCCGAATGTATCACGGAGAGAAAGCAGCAGAAGCTGCCGAACACTCGTTTAAAACAGTCTTTCAGGAAAACAGCCTGCCGGAAGATATACCGGCCGTAAACTGGAAAGGCGAAAAAACGATAGCGATGATTGATCTGCTCGTCAAGCTAAAGCTCCTCT  >  NC_000964/3947763‑3948253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑