Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	2,271,523	A→C	D45E (GAT→GAG)	uvrX ←	lesion bypass phage DNA polymerase; phage SPbeta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	2,271,523	0	A	C	100.0%	95.5 / NA	27	D45E (GAT→GAG)	uvrX	lesion bypass phage DNA polymerase; phage SPbeta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/14);  total (13/14)

GATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTCTGGTCTTTTACGTGAAGCTTTTGTTGTTCAAAATTTATGTAATGGACTCTGCC  >  NC_000964/2271308‑2271763                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa                                                                                                                                                                                                                >  1:288128/1‑250 (MQ=255)                                tctcAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATAc                                                                                                                                                                                 >  1:7966/1‑250 (MQ=255)                                     ggAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGc                                                                                                                                                                           <  1:280163/251‑1 (MQ=255)                                          aaaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCGAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTccc                                                                                                                                                                                                                                       >  2:106579/1‑186 (MQ=255)                                          aaaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTccc                                                                                                                                                                                                                                       <  1:106579/186‑1 (MQ=255)                                                  aaaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGAttt                                                                                                                                                               <  1:46765/250‑1 (MQ=255)                                                   aaaaaGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGTTACAGAAGCATAAAAGGATTTc                                                                                                                                                              >  2:207888/5‑250 (MQ=255)                                                    aaCAGCTTTTTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATATTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACTCTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCat                                                                                                                                                            <  2:94440/251‑1 (MQ=255)                                                       aGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATc                                                                                                                                                         >  1:14901/1‑251 (MQ=255)                                                           ttGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGa                                                                                                                                                       <  1:208220/249‑1 (MQ=255)                                                            tGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGaca                                                                                                                                                     <  1:318015/250‑1 (MQ=255)                                                            tGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCGACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACATAAGCATAAAAGGATTTCATATCGaca                                                                                                                                                     <  2:92605/250‑1 (MQ=255)                                                                                         gCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATtgtg                                                                                                                         >  1:147855/1‑249 (MQ=255)                                                                                              aTTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTaatc                                                                                                                  >  2:283610/1‑251 (MQ=255)                                                                                                 tgtgGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTaatcaa                                                                                                                <  2:181778/250‑1 (MQ=255)                                                                                                      aTTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCCAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATtg                                                                                                          >  1:235954/1‑251 (MQ=255)                                                                                                                        tGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCCGGAGATGCAGCTAATACTACTCTCCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTACAGGCTTAAGCCCCCTTGTTACAGCCTATACAGAAGCATAAAAGGATTTCATATCGCCTCACAGTATATTCTTTCGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTatgat                                                                                         <  2:312110/250‑1 (MQ=14)                                                                                                                             cTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTAtt                                                                                   >  1:249940/1‑251 (MQ=255)                                                                                                                                                     aTCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTc                                                           >  1:69546/1‑251 (MQ=255)                                                                                                                                                       ccTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTGGATATAAACTGTATTGTCAt                                                         <  1:82382/251‑1 (MQ=255)                                                                                                                                                         tGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTc                                                       >  1:305720/1‑251 (MQ=255)                                                                                                                                                                aTTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTCTGGTCtt                                                <  1:283610/251‑1 (MQ=255)                                                                                                                                                                    cAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTCTGGTCTGTTAc                                            <  1:207888/251‑1 (MQ=255)                                                                                                                                                                    cAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGACAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTCTGGTCTTTTAc                                            >  1:134628/1‑251 (MQ=255)                                                                                                                                                                                         gagatgaagataataataaacataacTGTCTCTCCGTATTACCTACAAAAGTTAGTTACCCTGTTAAAGGGTTAAACCCCATTGTTACAGCCGATACAGAAGCATCAAAGGATTTCGGATCGACACAAAGTATATTCTTACGTGGCAATAGTGAGTAATCAATCATGGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTTTCATTCTGGTCTTTTACTTGAAGCTTTTGTTGTTCaa                        >  2:203979/26‑250 (MQ=14)                                                                                                                                                                                                agccaatactacactTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTAGTCATGTTGATATAAACTGTATTGTCATGCTGGTCTTTTACGTGAAGCTTTTGTTGTTCAAAATTTATg                <  2:235954/244‑1 (MQ=255)                                                                                                                                                                                                               tCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTCTGGTCTTTTACGTGAAGCTTTTGTTGTTCAAAATTTATGTAATGGACTCTGcc  <  2:69546/250‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCATTGTATGTAACTCCTATGATGTTATTCATGTTGATATAAACTGTATTGTCATTCTGGTCTTTTACGTGAAGCTTTTGTTGTTCAAAATTTATGTAATGGACTCTGCC  >  NC_000964/2271308‑2271763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑