Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	1,195,173	A→G	H47R (CAT→CGT)	argC →	N‑acetylglutamate gamma‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	1,195,173	0	A	G	100.0%	125.4 / NA	35	H47R (CAT→CGT)	argC	N‑acetylglutamate gamma‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

ACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCATCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGTATAAACGGACAGCGGCACCGAAGGCGGTGATTCAAGAGGCGGTAT  >  NC_000964/1194927‑1195415                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aCAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCtt                                                                                                                                                                                                                                                <  2:247568/251‑1 (MQ=255)                                aaTATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGa                                                                                                                                                                                                                 <  1:98543/251‑1 (MQ=255)                                   ataCGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATAATCAGCTGAAGCCGAtt                                                                                                                                                                                                               <  1:243715/250‑1 (MQ=255)                                     aCGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGat                                                                                                                                                                                                            >  2:219629/1‑251 (MQ=255)                                       gATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGAGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGCGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGatat                                                                                                                                                                                                          >  2:242812/1‑251 (MQ=255)                                                      tATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAac                                                                                                                                                                                            >  1:49620/1‑250 (MQ=255)                                                          cATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGa                                                                                                                                                                                        >  1:186429/1‑250 (MQ=255)                                                           aTGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGTGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGaa                                                                                                                                                                                       >  2:157272/1‑250 (MQ=255)                                                                 taATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGat                                                                                                                                                                                 >  1:200425/1‑250 (MQ=255)                                                                                   caAAGACCAAGAGGGTTAACTTGTTTTGAAAATAGGAATTGTCGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTgcgc                                                                                                                                                              <  2:123721/251‑1 (MQ=255)                                                                                      aGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGcgccgc                                                                                                                                                           >  2:37841/1‑251 (MQ=255)                                                                                       gACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATATTTCTCGCTGCgccgcc                                                                                                                                                          >  1:258135/1‑251 (MQ=255)                                                                                        aCCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGccc                                                                                                                                                         >  1:209349/1‑251 (MQ=255)                                                                                               gggTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTAt                                                                                                                                                  <  2:270466/251‑1 (MQ=255)                                                                                                              gAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGAGTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCGATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACt                                                                                                                                    <  2:265713/250‑1 (MQ=255)                                                                                                                      gAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGCTTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTg                                                                                                                           >  2:157579/1‑251 (MQ=255)                                                                                                                            tAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGAc                                                                                                                     >  2:337254/1‑251 (MQ=255)                                                                                                                               gTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTtct                                                                                                                                                                      <  2:338624/199‑1 (MQ=255)                                                                                                                               gTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTtct                                                                                                                                                                      >  1:338624/1‑199 (MQ=255)                                                                                                                                        gATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACg                                                                                                         <  1:144225/251‑1 (MQ=255)                                                                                                                                                             tCAGGATTCTTTCGCATCATCCTCATGCAGCGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGAt                                                                                    >  1:51956/1‑251 (MQ=255)                                                                                                                                                               aGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTg                                                                                             >  2:311512/1‑240 (MQ=255)                                                                                                                                                               aGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTg                                                                                             <  1:311512/240‑1 (MQ=255)                                                                                                                                                                ggATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTg                                                                                 >  1:152234/1‑251 (MQ=255)                                                                                                                                                                ggATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTg                                                                                 <  2:186429/251‑1 (MQ=255)                                                                                                                                                                          gCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAg                                                                       <  1:229864/251‑1 (MQ=255)                                                                                                                                                                                    cATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTg                                                              >  2:79173/1‑250 (MQ=255)                                                                                                                                                                                         agagGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGATTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATAAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATg                                                        >  1:244975/1‑251 (MQ=255)                                                                                                                                                                                                   aTACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATATTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGta                                              <  2:258135/251‑1 (MQ=255)                                                                                                                                                                                                             cATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGTATAAACGGACa                                    <  1:242812/251‑1 (MQ=255)                                                                                                                                                                                                                      gagaAGGGAATGTCTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGACGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAAACGGCTGAATATGAAAAATGGTATAAACGGACAGCggaaacg                           >  2:991/1‑246 (MQ=255)                                                                                                                                                                                                                        gaAGGGACTGTCCATAACGGGGGTTATCCTCGTCTTAACGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATTTGAATACGATCAAACACGAAATAGATATCCTGTTTCTCGCTGCGCGGCCAGGAGTATCAAGTGAATTGACTGCAACGCTGGCAGACGCGGGAATTACGGTTATTGAACTGTAAGGTGATCTGTGAATAAAAGAACCGGCGGAATATGAAAAATGGCATAACCGGACAGTGGCACCgc                          >  2:126738/1‑249 (MQ=255)                                                                                                                                                                                                                                   cTATAGCGAGGGTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGTATAAACGGACAGCGGCACCGAAGGCGGGGATTc              >  2:298488/1‑251 (MQ=255)                                                                                                                                                                                                                                             ggTTATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGTATAAACGGACAGCGGCACCGAAGGCGGTGATTCAAGAGGCGGt    >  2:103106/1‑251 (MQ=255)                                                                                                                                                                                                                                                tATCCTCGTCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGTATAAACGGACAGCGGCACCGAAGGCGGTGATTCAAGAGGCGGtat  <  1:37841/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTCTATAGCGAGGGTTATCCTCATCTTACCGGCTTAGCGGATCAGCAGCTGAAGCCGATTGATATGAATACGATCAAACACGAAATAGATATCATGTTTCTCGCTGCGCCGCCCGGAGTATCAAGTGAATTGACTCCAAAGCTGGCAGACGCGGGAATTACGGTTATTGATCTGTCAGGTGATCTGAGGATAAAAGAACCGGCTGAATATGAAAAATGGTATAAACGGACAGCGGCACCGAAGGCGGTGATTCAAGAGGCGGTAT  >  NC_000964/1194927‑1195415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑