Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	1,176,825	A→G	L143L (CTT→CTC)	yitG ←	putative efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	1,176,825	0	A	G	96.7%	100.2 / NA	30	L143L (CTT→CTC)	yitG	putative efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (11/18);  minor base T (0/1);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TAAGCGGTAAACAGCCATCTGCCTTCATGTTTAAAGACACTTAACAGCCCTTTTGCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACAAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGATAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATGACTTTCCTTGAGAATCGGTCTGCGAGATAACCGACAATCGGA  >  NC_000964/1176586‑1177068                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    aaagagggaaaCAGCCAACTGCCTTCATGTTTAAAGACACTTAACAGCCATTTTGCATATTGGCCGATTGGTGGCGGTTATTTGTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACATGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTCCCCCATGCCGTTTGAAGCTTAAACGAGGCCGCGCAc                                                                                                                                                                                                                                          <  2:208810/243‑1 (MQ=255)       gTAAACAGCCATCTGCCTTCATGTTTAAAGACACTTAACAGCCCTTTTGCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGct                                                                                                                                                                                                                                    <  1:270406/251‑1 (MQ=255)                     gCCTTCATGTTTAAAGACACTTAACAGCCCTTTGGCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCt                                                                                                                                                                                                                       <  2:194271/250‑1 (MQ=255)                          cATGTTTAAAGACACTTAACAGCCCTTTTGCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGCAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAACGAGCCAATGATCGGCCATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGGGCACCTTGCTCTCCTGGGCGCCTTTAAAc                                                                                                                                                                                                                 >  2:128869/1‑251 (MQ=255)                                             cAGCCCTTTTGCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTTTTTTCTTTAATAAAAATCCAAGTCAGTCCGAGCGAAAAGATACAAAACACAGGAAAGGCAAAAAAAGCGCCATACCCGACAAGCCAGGCGGTCAGCGAGCCAATGATCGGCGGTAGCACTTTCCCCATGCCGCTTGAAGCTTCAACGAGGCCGAGCACCCTGCTCCCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGccc                                                                                                                                                                                              >  1:176900/1‑250 (MQ=255)                                               gCCCTTTTGCATATTGGCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATg                                                                                                                                                                                            <  2:158517/251‑1 (MQ=255)                                                    tttGCATATTGTCCGATTGGTGGCGGTTCTTTTTTCTTTTTTTTTTCTTTTATAAAAATCCAAGTCAGTACGGTCGAAATTATACAAAACAAAGGAAAAGCAAAAAAAGCGCCATACCAAAAAAGCAAGGGGAACAGCGAGACAATGATCGGCGAACACACTTTTCCCATCCCGCTTTAAGCCTCAAAGAAGCCGAGCAACTTGCTCTCCCGGGCGCCTTTAAACACATCCCCCAGCAGCGCCATGGccat                                                                                                                                                                                       >  2:181753/1‑248 (MQ=255)                                                       gCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTgg                                                                                                                                                                                    <  1:134689/251‑1 (MQ=255)                                                                     tggtggCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCt                                                                                                                                                                       <  1:116038/250‑1 (MQ=255)                                                                         ggCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGcccc                                                                                                                                                                  <  1:128869/251‑1 (MQ=255)                                                                                    ttcctttttcTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGt                                                                                                                                                        >  2:221903/1‑250 (MQ=255)                                                                                    ttcctttttcTTTTCTTTAAAAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGt                                                                                                                                                        <  2:22075/250‑1 (MQ=255)                                                                                           ttcttttcttTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTc                                                                                                                                                 <  1:304165/250‑1 (MQ=255)                                                                                             cttttctttAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTc                                                                                                                                              >  1:166876/1‑251 (MQ=255)                                                                                              ttttctttAATAAAAAGCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCCGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAGCGCTCGTc                                                                                                                                              <  1:15895/250‑1 (MQ=255)                                                                                               tttctttAATAAAAATCCAAGTCAGTCCGATCGAAATGATACAAACCACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTc                                                                                                                                              <  2:187197/249‑1 (MQ=255)                                                                                               tttctttAATAAAAATCCAAATCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAACCGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTcc                                                                                                                                             <  2:184396/250‑1 (MQ=255)                                                                                                ttctttAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCt                                                                                                                                            <  1:178751/250‑1 (MQ=255)                                                                                                   gttAATAAAAATACAAGTCAGTACGATCGAAATGAAACAAAACCCAGGAAATGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCGGTCCCGATTCCCTGTAACGCTCGTCCTGcc                                                                                                                                         <  2:99540/249‑1 (MQ=255)                                                                                                             aTCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAgg                                                                                                                              <  2:67314/251‑1 (MQ=255)                                                                                                                  aGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATAAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATTGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAgg                                                                                                                         >  2:159148/1‑251 (MQ=255)                                                                                                                              cGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCaaaa                                                                                                              <  1:259030/250‑1 (MQ=255)                                                                                                                                           accacaGGAAAGCAAAAAAACGCCCCATACCGAACAACCAGGCCGACAAGCGAGCCAATGACCGGCGAACGCACTTTCCCCATCCCGTTTGAACCTCAAACTAGGCCGAGCCCCTTGCTCTCCTGGGCGCCTTTAACCAGATCTCCCGTCATTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATCCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAAACCTGCCGCaaa                                                                                                <  2:26420/249‑1 (MQ=255)                                                                                                                                                                      tACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGCTAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCAt                                                                      >  1:158672/1‑250 (MQ=255)                                                                                                                                                                                         ccAGCGAGCAAATGATCGGCGATAGCACTTTCCCCATGCCTTTTGAAGCTCCACCGAGGCCGACCACCTTGCTCTCCGGGGCGCTTTTAAACAGATCCCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTACCGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGaata                                                  <  1:6299/250‑1 (MQ=255)                                                                                                                                                                                                  cAATGACCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATGACt                                         >  2:44603/1‑251 (MQ=255)                                                                                                                                                                                                                aGCACTTTCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATGACTTTCCTTGAGAATCg                           >  2:230615/1‑251 (MQ=255)                                                                                                                                                                                                                       tCCCCATGCCGTTTGAAGCTTCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGTCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCACGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATGACTATCCTTGAGAATCGGACTGCg                    >  2:291378/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGAAGCTCCAACGAGGCCGGGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAACCCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATGACTTTCCTTGAGAATCGGTCTGCGAGATAACCGACa        <  1:138906/250‑1 (MQ=255)                                                                                                                                                                                                                                          ttCAACGAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGGTAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATAACTTTCCTTGAGAATCGGTCTGCGAGATAACCGACAATCGGa  >  2:161478/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    TAAGCGGTAAACAGCCATCTGCCTTCATGTTTAAAGACACTTAACAGCCCTTTTGCATATTGTCCGATTGGTGGCGGTTCTTTTTCCTTTTTCTTTTCTTTAATAAAAATCCAAGTCAGTACGATCGAAATGATACAAAACACAGGAAAGGCAAAAAACGCGCCATACCAGACAAGCAGGGCGATCAGCGAGCCAATGATCGGCGATAGCACTTTCCCCATGCCGTTTGAAGCTTCAACAAGGCCGAGCACCTTGCTCTCCTGGGCGCCTTTAAACAGATCTCCCGTCAGTGCCATGGCAATTGGGCCGGTTCCGGCTGCCCCGATTCCCTGTAACGCTCGTCCTGCCATGACCCAAGGATAGGCATTGTCAAAAAAACCTGCCGCAAACCCGGCTAACAAACCGCCGGCACCATACAAAATTAAGCAGGGAATAATGATGACTTTCCTTGAGAATCGGTCTGCGAGATAACCGACAATCGGA  >  NC_000964/1176586‑1177068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑