| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000964 | 3,847,273 | 0 | C | T | 45.5% | 8.8 / 16.1 | 22 | intergenic (+24/‑75) | phrF/ywhH | secreted regulator of the activity of phosphatase RapF/putative tRNA editing enzyme |
| Reads supporting (aligned to +/- strand): ref base C (1/11); new base T (10/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCTGTCCTTCAGAGGGGAAGGAGATCAGGTGATTCTGAT > NC_000964/3847043‑3847523 | aTGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTTGTCCCCCCTTTACCacaaac > 1:157343/1‑246 (MQ=255) gAACAAGTAAGGCAACTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑ATTTTTTGTTCCCCCTTTACCCAAAACTCCCCAACCCTGCTATTATTTTTTTTATTATCTTTAAGAGAGTGGa > 2:40821/1‑250 (MQ=255) aGGCAACTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑CTTTTTTGTCCCCTCCTTTTCAAAATCTTCCCATCCAAGCAATTATTTTTTTAATAAACTTTTAGAGAATGGAAAAgcagca > 2:83570/1‑250 (MQ=255) cAACTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑GTTTTTTGTCCCCCCCTTTCCCAAGTCTCCCCATCCAAGCTATTATCTTTTTTATAATTTTTAAGAGAGGGGGAATGCAGCATGtt > 1:63970/1‑250 (MQ=255) cTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTTGGCCCCCCTTTTCCAAAATCTCCCAATCCCTTCTATTATTTTTTTAATAATCTTTAAAAGAGTGGAAATACAGCATGTctct > 1:252587/1‑251 (MQ=255) tCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTaaaa < 2:36261/250‑1 (MQ=255) cAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCGCCCAATCCATGCTATGATCTTTTCAATAATCTTGGAGAGAGTGGAAATGCAGCATGTCGCTAAAAAg < 1:169571/251‑1 (MQ=255) gaggagTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGtgtg < 2:29568/251‑1 (MQ=255) tgagtTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTTGTTCCCTCCTTTCCAAAGTCTCCCAATCCATGCTATGAACTTTTTCATAATTTTTAAGAGAGAGGGAATGCAGCCAATCTCTaaaaaaagtgggaaac > 1:323927/1‑240 (MQ=255) gAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGGGTTTTTTTTTCCCCCTTTTCCCAAATCCTCCCATCCCGTGTTAT‑ATCTTTTTTCTAATCTTTAAGAGGGGGGAAATGACGCATGTCTCTAAAAAATGGGAGAACCCAATTTTCTCAAAGGa > 1:38049/1‑250 (MQ=255) gTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTTTTTCCCCCTTTTTCAAAAACTCCCAAACCCCACTAAGGGCTTTTCAATAATCTTGAAGGGAGGGGAAAATCAACAAGACTCTAAAAAATGGGAGAacacaccttgttccaatgaaga > 1:232591/1‑234 (MQ=255) tctcaaCTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTTGTCCCCCCCTTTCCAAAGTCTCCCAATCCCAGCCTTGTTCTTTTTTATAATCTTTAAGAGAGTGGTAATGCAGCATGTCTCTAAAAAAGGTGGGAAACCACtttttttaattggaa > 2:295650/5‑236 (MQ=255) tAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTgg < 2:325222/169‑1 (MQ=255) caacTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAAACTCCCAATCCATGCTATGATCTTTTTTATAATCTTTAAGAGAGGGGGAATGCAGCATGTCTCTAAAAAATGTGAGAAACCACTTTACTCAAAGGAATCGAGAAAAAAATGTGACGGAGGTCGAAACGTCGAGGGGGACAGGTGAACAgg > 1:215158/1‑251 (MQ=255) tGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTTGTCCCCCCTTTTCCAAAAGCTCCCAATCCATGCTATGATCTTTTTAATAATCTTTAAGGGAGGGGGAAAGGAGCAAGTCTCTAAAAAATGTGAGAACCCACTTTACTCAATAGAAATGAGAAAAAGATGTGACGGAGGTCGAAAAGGCGGGGGCGGCAGGTGAAAAGGCAGCTgg > 1:142552/1‑250 (MQ=255) gCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATc < 1:4262/251‑1 (MQ=255) acacCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAg < 1:5098/251‑1 (MQ=255) cGAGGAATGATTTAACCGCCGTCCATCGGCTG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTcc < 2:93610/251‑1 (MQ=255) aGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGGCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCt < 2:291773/251‑1 (MQ=255) ccgTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCTGTCCTTCAGAGGGGaa < 2:142552/251‑1 (MQ=255) ccATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCTGTCCTTCAGAGGGGAAGgag < 1:348854/251‑1 (MQ=255) gg‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCTGTCCTTCAGAGGGGAAGGAGATCAGGTg < 1:273292/251‑1 (MQ=255) cGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCTGTCCTTCAGAGGGGAAGGAGATCAGGTGATTCTGAt < 2:313581/251‑1 (MQ=255) | ATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAGTGAGTTTGTATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACCTACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAACCGCCGTCCATCGGCGG‑TTTTTTCGTCCCCTCTTTACCAAAGTCTCCCAATCCATGCTATGATCTTTTCAATAATCTTGAAGAGAGTGGAAATGCAGCATGTCTCTAAAAAGTGTGAGAACCCACTTTACTCAATGGAATCGAGAAAATGATGTGACGGAGTTCGAAACGTCGAGTGCGACAGTTGAACAGGCAGCTGAGACAATCGGCGTAAGCCTGTCTAGAATCGCCAAGTCCCTGTCCTTCAGAGGGGAAGGAGATCAGGTGATTCTGAT > NC_000964/3847043‑3847523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |