Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	3,788,769	0	T	C	41.7%	17.5 / 59.8	48	K96R (AAA→AGA)	upp	uracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (15/13);  new base C (10/10);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TATCAACGTCCGAATGATGCTTCTGCAATTCTTCCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTATTTTCATTCCGTATATATGTCAGCTTGTGCTG  >  NC_000964/3788521‑3789019                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                           tatCAACGTCCGAATGATGCTTCTGCAATTCTTCCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACtttt                                                                                                                                                                                                                                                          >  2:125578/1‑251 (MQ=255)    caccttCCGAATGATGCTCCTGAAATTCTCCCCCCCCCCCCGGCCCTCCTACAAGACCCATAAACCGGATTTTTTTCCCCCCGCGTTTTTAAAGGCTGTTAATG‑GCTCCACCT‑GCGAAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGcc                                                                                                                                                                                                                                                       <  1:26040/246‑1 (MQ=255)    cAACGTCCGAATGATGCTTCTGCAATTCTTCCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGcc                                                                                                                                                                                                                                                       >  2:348215/1‑251 (MQ=255)      aCGTCCGAATGATGCTTCTGCAATTCTTCCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGc                                                                                                                                                                                                                                                     >  1:242563/1‑251 (MQ=255)       cGTCCGAATGATGCTTCTGAAATTCTTCCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGAGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGCTGAATTCACGCTCTTCCACATCAGAAGGACGCTTGACATCGTATTACACGGGATTTAAGGTTTCTGGATCCCGGAAAGGGCCGACATGTCCCACTTTTGCCGc                                                                                                                                                                                                                                                     >  2:338036/1‑250 (MQ=255)           cGAATGATGCTTCTGCAATTCTTCCACACCATCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGCCATGTCCCACTTTTGCCGCAGGa                                                                                                                                                                                                                                                 >  2:231857/1‑250 (MQ=255)                       cTGCAATTCTTACACACCCTCAGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAGTTCACGCTCTTCCACATCAGAATGAAGCTAGACATATCATTCCCCGGTTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACCTGTCCCACTTCTGCCGCAGGACTCAGCTTTaaa                                                                                                                                                                                                                                     >  2:179336/1‑250 (MQ=255)                               cttcCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTc                                                                                                                                                                                                                             >  1:35767/1‑250 (MQ=255)                                  ccACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGTTTTAAAATGCCGTCAAcc                                                                                                                                                                                                                         >  2:242883/1‑251 (MQ=255)                                                      aCAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTg                                                                                                                                                                                                       >  1:67006/1‑249 (MQ=255)                                                      aCAAGACACATGAAACGGATATTTTCCGCCCCGCGTTTTTAAAGGCTGTAAAGG‑GTTTAAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAg                                                                                                                                                                                                     <  2:55305/251‑1 (MQ=255)                                                                  aaaCGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCAc                                                                                                                                                                                         >  2:302533/1‑251 (MQ=255)                                                                     cGGATCTTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTACACG‑GAGGAACAGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATCGGAACCACTcc                                                                                                                                                                                      >  2:121116/1‑251 (MQ=255)                                                                      ggtttttttcCGCCCCGCTTTTTTTAAGGCTGTGAATG‑GCTTCAACG‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTcc                                                                                                                                                                                      <  1:90011/245‑1 (MQ=255)                                                                      ggATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGCGGATAGGAACCACTcc                                                                                                                                                                                      >  2:90011/1‑250 (MQ=255)                                                                      ggATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCg                                                                                                                                                                                     >  1:205578/1‑251 (MQ=255)                                                                            ttttCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGtttt                                                                                                                                                                               >  1:217342/1‑251 (MQ=255)                                                                             ttccacACCGCGTTTTTTAAGGCGGTGA‑TGTGCT‑ATACTCGCGGACCCGCCTGTAGCGAGCGTCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCCCGGTAAAGGCCGACATGTCCCCCTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGtttt                                                                                                                                                                               <  2:242563/245‑1 (MQ=255)                                                                                        gTTTTTAAAGGCTGTAAATG‑GCTTCAACG‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGAt                                                                                                                                                                    <  1:343322/250‑1 (MQ=255)                                                                                            tttAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACtt                                                                                                                                                               >  1:2184/1‑251 (MQ=255)                                                                                               agggCTGTGAATG‑GCTTCAACG‑GCGAAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCg                                                                                                                                                            <  1:114535/249‑1 (MQ=255)                                                                                                 ggttgtgAATG‑GCTTAACCG‑GAGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGAt                                                                                                                                                          <  2:319966/248‑1 (MQ=255)                                                                                                        aagg‑gCTTCAACG‑GCGAACCCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAg                                                                                                                                                   <  2:2184/248‑1 (MQ=255)                                                                                                        aaTG‑GCTTCACCT‑GCGGAACCGACTGTAGCGAGCATCGGGTCAACCACGCTGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCa                                                                                                                                                    >  2:270692/1‑250 (MQ=255)                                                                                                             gCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTg                                                                                                                                               >  1:69587/1‑251 (MQ=255)                                                                                                                           acccGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTg                                                                                                                                  <  2:117736/249‑1 (MQ=255)                                                                                                                           aaCCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTg                                                                                                                                  >  2:157276/1‑251 (MQ=255)                                                                                                                               gCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCATCCTGAACCGGTGTATTGatat                                                                                                                              <  2:113824/251‑1 (MQ=255)                                                                                                                                     aGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCAct                                                                                                                         <  1:342151/250‑1 (MQ=255)                                                                                                                                      gCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCActtc                                                                                                                       >  1:135369/1‑251 (MQ=255)                                                                                                                                            aTCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCag                                                                                                                 <  1:348215/251‑1 (MQ=255)                                                                                                                                              cGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTTTATTGATATCCACTTCTTCCaga                                                                                                                <  2:117352/250‑1 (MQ=255)                                                                                                                                                                           ccACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATg                                                                                  <  1:302533/251‑1 (MQ=255)                                                                                                                                                                           ccACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTGTTAAGGTTGCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTCCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATg                                                                                  <  2:98117/251‑1 (MQ=255)                                                                                                                                                                                  aGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAgtgt                                                                             >  1:135606/1‑249 (MQ=255)                                                                                                                                                                                   gaaggaagCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGCGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGc                                                                          >  2:10387/1‑251 (MQ=255)                                                                                                                                                                                    aaggaagCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGcc                                                                         <  2:53277/251‑1 (MQ=255)                                                                                                                                                                                         agCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTc                                                                    >  2:58353/1‑251 (MQ=255)                                                                                                                                                                                              gACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATct                                                                <  1:17236/250‑1 (MQ=255)                                                                                                                                                                                              gACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATcta                                                               <  1:251795/251‑1 (MQ=255)                                                                                                                                                                                                   aGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTc                                                                    <  2:175974/241‑1 (MQ=255)                                                                                                                                                                                                   aGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTc                                                                    >  1:175974/1‑241 (MQ=255)                                                                                                                                                                                                      aTTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAActct                                                       >  2:243712/1‑251 (MQ=255)                                                                                                                                                                                                                  ttAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTCTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCtt                                            <  1:445/250‑1 (MQ=255)                                                                                                                                                                                                                              gATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTAttt                               >  1:281673/1‑251 (MQ=255)                                                                                                                                                                                                                                 cACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTCTCCCTGAGATGACTTTCGATTTCGGAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTATTTTc                             <  2:35767/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaaGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTATTTTCATTCCGt                      <  2:114135/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGGCCGACATGTCCCCCTCTTGACGCAGGAATCTGTTTTAAAATGCCGACAACCATTCCCAATTCTGCGATGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGCCTTTCGATTTCGAAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGCAGATCGCGGGTAATTTCAAATGCCATGTGTGTAGCCACTTCATCTACTAAATCGCTAACATACTTCGTACGTGTATTTTCATTCCGtat                    >  2:296159/1‑251 (MQ=255)                                                                                                                                                                                                                                              gACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCGAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTATTTTCATTCCGTATATATg               <  2:32079/251‑1 (MQ=255)                                                                                                                                                                                                                                                           tttGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTATTTTCATTCCGTATATATGTCAGCTTGTGCTg  >  2:204640/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                           TATCAACGTCCGAATGATGCTTCTGCAATTCTTCCACACCCTCCGGCGCTGCTACAAGACACATGAAACGGATATTTTTCGCACCGCGTTTTTTAAGGCTGTGAATG‑GCTTCAACT‑GCGGAACCGCCTGTAGCGAGCATCGGGTCAACCACGATGAATTCACGCTCTTCCACATCAGAAGGAAGCTTGACATAGTATTCCACGGGTTTTAAGGTTTCTGGATCACGGTAAAGGCCGACATGTCCCACTTTTGCCGCAGGAATCAGCTTTAAAATGCCGTCAACCATTCCCAATCCTGCTCTGAGGATAGGAACCACTCCGAGTTTTTTCCCTGAGATGACTTTCGATTTCGCAGCCTGAACCGGTGTATTGATATCCACTTCTTCCAGAGGAAGATCGCGGGTAATTTCAAATGCCATGAGTGTAGCCACTTCATCTACTAACTCTCTAAAATCCTTCGTACCTGTATTTTCATTCCGTATATATGTCAGCTTGTGCTG  >  NC_000964/3788521‑3789019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑