Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	1,154,631	2	.	T	20.5%	95.5 / 33.7	39	coding (843/2685 nt)	wprA	cell wall‑associated protease
Reads supporting (aligned to +/- strand):  ref base . (16/15);  new base T (6/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAGCTGAGCCAAACAGCAGAAGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGAAAAAAGACATATCTAATGTGAAAAAGGCAA  >  NC_000964/1154381‑1154866                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            agcagcTGAGCCAAACAGCAGAAGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTc                                                                                                                                                                                                                                               <  1:884/251‑1 (MQ=255)               aaagtagAAGGAAAAGAACTGACAAGCCATTAGTACAAAACAGCACCGTTTATCGTTGCAAGGCTGGCACTAAATAAAAAAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACAATGTGTCAGAAAACGGAGCAGCATCTTCGTATAAGAACACTGAAAAGGAGCAAAAAGCGATCAACGGGAAATATGATAAAGCTTAACAATCAGTCCCGTCATTC‑‑ATAAAAgaggagcc                                                                                                                                                                                                                               >  2:316873/6‑249 (MQ=255)                cagcagAAGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAATATGAGATa                                                                                                                                                                                                                              <  1:255990/251‑1 (MQ=255)                      aaGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGaa                                                                                                                                                                                                                         >  2:128394/1‑250 (MQ=255)                        ggCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTCTTAAAGAGTAGATAAAGaaa                                                                                                                                                                                                                        <  1:44914/251‑1 (MQ=255)                         gCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACaa                                                                                                                                                                                                                     <  2:195180/251‑1 (MQ=255)                           aaaGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTCTTAAAGAGGAGATAAAGAAACa                                                                                                                                                                                                                      >  1:159339/1‑250 (MQ=255)                               aaCTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTCTTAAAGAGGAGATAAAGAAACAAGCgg                                                                                                                                                                                                                 >  1:51381/1‑251 (MQ=255)                                       aGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTaaa                                                                                                                                                                                                       <  2:54505/251‑1 (MQ=255)                                       aGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTaaa                                                                                                                                                                                                       <  1:342608/251‑1 (MQ=255)                                        gcctttagtgcaagggagcaCCGTTTATCGTTGCAAATCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGTTCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGTCTATATGATAAAGCTTTACAATCCGTCCCGTCATTC‑‑CTTAAAGATGCGATAAAGAAACAAGCGGACCGACTAAat                                                                                                                                                                                                      <  2:12115/235‑1 (MQ=255)                                          cTTTATTACAAAGCAGCCCCGGTTATCGTTTCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGAGGTGTCAGAAAACAGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACGGGCTATATGATAAAGCTTAACAATCAGTCCCGACATTCCTCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAat                                                                                                                                                                                                      >  1:136735/1‑251 (MQ=255)                                                 aCAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGCCTCAATATGAagc                                                                                                                                                                                               >  2:144933/1‑249 (MQ=255)                                                                    cGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAAcagc                                                                                                                                                                            >  2:277386/1‑249 (MQ=255)                                                                      ttGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAgg                                                                                                                                                                         >  1:277354/1‑250 (MQ=255)                                                                           aaGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCAt                                                                                                                                                                   <  1:128713/251‑1 (MQ=255)                                                                             gCTCGCACTCAATAAAACAGCAAGAAAGGAAAGCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCATGGCAAAACAGCAGGAGCCAttt                                                                                                                                                                 <  2:47304/251‑1 (MQ=255)                                                                                  cACTCAATAAAACAGCAAGAACTGAAATCTATCAGGATCTTGAGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGCGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTACCGTAATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAAc                                                                                                                                                             >  2:233864/1‑250 (MQ=255)                                                                                       aaTAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGaaa                                                                                                                                                        >  1:71210/1‑250 (MQ=255)                                                                                              cAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCTGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACTGCAGGAGCCATTTTAACAGAAAACAATAtt                                                                                                                                                <  1:308428/251‑1 (MQ=255)                                                                                                            atCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTg                                                                                                                                   >  2:43700/1‑250 (MQ=255)                                                                                                                  cagtaTCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGACATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGtta                                                                                                                             <  2:24166/246‑2 (MQ=255)                                                                                                                  cAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTc                                                                                                                             >  1:281852/1‑250 (MQ=255)                                                                                                                   aGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTc                                                                                                                             >  1:86964/1‑249 (MQ=255)                                                                                                                     gATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCATCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGACAACAATATTGCAGCAAAAAGTGAAGTTc                                                                                                                             >  2:139593/1‑249 (MQ=255)                                                                                                                     gATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGac                                                                                                                         >  2:159513/1‑251 (MQ=255)                                                                                                                       tCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGac                                                                                                                         >  2:201349/1‑251 (MQ=255)                                                                                                                          tgtgACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGac                                                                                                                         <  2:71210/246‑1 (MQ=255)                                                                                                                          tgtgACATTAAAGACGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGac                                                                                                                         <  2:36503/246‑1 (MQ=255)                                                                                                                                          ttATTTGACGGTGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGAGAAGTTCAGACAACAACGGTTATTTTCAAGGt                                                                                                    >  2:73989/1‑251 (MQ=255)                                                                                                                                                                 cGGAGCATCATCTTCGTATAAGGTCACTGAAAAGAATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGAAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCAt                                                                             <  2:200251/251‑1 (MQ=255)                                                                                                                                                                   gAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCCTCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCAt                                                                             <  1:201349/251‑1 (MQ=255)                                                                                                                                                                                  ataAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATaatgaaatg                                                            >  2:215304/1‑251 (MQ=255)                                                                                                                                                                                         cACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGc                                                      <  1:128394/250‑1 (MQ=255)                                                                                                                                                                                                aaGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGc                                              >  1:265883/1‑251 (MQ=255)                                                                                                                                                                                                                     cGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTCATCTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGaaaaa                            >  1:198415/1‑250 (MQ=255)                                                                                                                                                                                                                     cGGCTATATGATAAAGCTTTACAATCAGGCCCGTCCTTC‑‑ATTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAGGCAGCTGCAAGGAAAATCAACAGGAGCCATTTTAACAGAAAACAATAGTGCATCAAAAAGTGAAGTCCAGACAACAAAGGTTAGTTTCAAGGTGAAGGACAATAAAAGCCTCGCAACCGTACATAATGAAATGAAGGGCTTGTCTTCAATCTCGCAACCGaaaaagga                         <  2:63810/251‑4 (MQ=255)                                                                                                                                                                                                                            atGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGAAAAAAGACATATc                   <  1:119846/250‑1 (MQ=255)                                                                                                                                                                                                                                           aaTCAGACCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCACGGTGAAGGACAATAAAAGCCTCTCATCCGTACATCATGAAATAAAGGTCTTTTCTGCAAGCGCGCAATCGAAAAAAGACAAATCAAATATAAAAAAGGc    >  2:10068/1‑250 (MQ=255)                                                                                                                                                                                                                                              cAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGAAAAAAGACATATCTAATGTGAAAAAGGCaa  >  1:181478/1‑249 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            AGCAGCTGAGCCAAACAGCAGAAGGCAAAGAACTGACAAGCCTTTATTACAAAGCAGCACCGTTTATCGTTGCAAAGCTCGCACTCAATAAAACAGCAAGAAATGAAATCTATCAGGATCTTGTGACATTAAAGCCGTTATTTGACGATGTGTCAGAAAACGGAGCATCATCTTCGTATAAGGTCACTGAAAAGGATCAAAAAGCGATCAACCGGCTATATGATAAAGCTTTACAATCAGTCCCGTCATTC‑‑CTTAAAGAGGAGATAAAGAAACAAGCGGACCGACTAAATATGAAGCAGCTGCAAGGCAAAACAGCAGGAGCCATTTTAACAGAAAACAATATTGCAGCAAAAAGTGAAGTTCAGACAACAAAGGTTATTTTCAAGGTGAAGGACAATAAAAGCCTCTCATCCGTACATAATGAAATGAAGGGCTTTTCTGCAAGCGCGCAATCGAAAAAAGACATATCTAATGTGAAAAAGGCAA  >  NC_000964/1154381‑1154866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑