Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	608,219	(A)5→6	intergenic (‑144/+27)	ydgF ← / ← dinB	putative amino acid permease/nuclease inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	608,219	1	.	A	100.0%	48.2 / NA	14	intergenic (‑144/+27)	ydgF/dinB	putative amino acid permease/nuclease inhibitor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (7/7);  total (7/7)

CCGCCGATGGCGATGAGCTGGATATGCCTGTTTTTCAAGCCTCTCTGCAACGTTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAA‑GCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGATTGACAACGTGGGGAAGCAGTTCGGAAACAGTTGTTTTCATGATTCCGCTTGAGGGATTTTGAATCTGGAGTGGCTTATTGAGCTGTTCTTTT  >  NC_000964/607984‑608453                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           ccgccgATGGCGATGAGCTGGATATGCCTGTTTTTCAAGCCTCTCTGCAACGGTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCAttt                                                                                                                                                                                                                                <  2:200552/249‑1 (MQ=255)                                                 aaCGTTTGCTGATTTATATTGTCTTTCGTCATGTAGTATGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAACAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAACCAGGTag                                                                                                                                                                                >  2:280840/1‑249 (MQ=255)                                                    gTTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGccc                                                                                                                                                                           <  1:134484/251‑1 (MQ=255)                                                             tttATATTCTCCTACGTCATGCCATCTGCCACTTTATGTGGTCCTCCATTGACAATTGTTTATTGAGTCGGCCATCTATTTCTCCTGATTATTTTACAACTAAAATTTCTATCTTTTTTCCTAACAAAGGATTGATGAACAGAATTTTAATGTGATTTGGCCTATTTCCCGAAAAAAGCGGCGCCATTAAGAGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGt                                                                                                                                                                  >  2:114239/1‑251 (MQ=255)                                                                            gTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTCTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTa                                                                                                                                                   <  1:328068/251‑1 (MQ=255)                                                                                              ttATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCgcagca                                                                                                                                  <  1:178038/250‑1 (MQ=255)                                                                                                       cctccATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGa                                                                                                                         <  1:308719/250‑1 (MQ=255)                                                                                                                             gAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTACAAAAGGATTGATGAAAAGCATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCg                                                                                                  >  2:260697/1‑251 (MQ=255)                                                                                                                                                aaTGATTATTATAACAATAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTGCCCGAAAAAAGCGGCTCCAGTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGTTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCTGCGGTGGTATGTCCCGTGATTGACAACGTGGGGa                                                                                <  1:229510/250‑1 (MQ=255)                                                                                                                                                       attatAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGGGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTTCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGATTGACAACGTGGGGAAGCAGTTc                                                                        <  1:77855/251‑1 (MQ=255)                                                                                                                                                                   aaaTTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTCTGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGATTGACAACGTGGGGAAGCAGTTCGGAAACAgttgg                                                            >  1:2126/1‑250 (MQ=255)                                                                                                                                                                      tttCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGATTGACAACGTGGGGAAGCAGTTCGGAAACAGTTGtttt                                                         >  1:148573/1‑251 (MQ=255)                                                                                                                                                                                                     gaAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGCTTGACAACGTGGGGAAGCAGTTCGGAAACAGTTGTTTTCATGATTCCGCTTGAGGGATTTTGAATCTgg                          >  2:55034/1‑251 (MQ=255)                                                                                                                                                                                                                             aCTATTTCCCGAAAAAAGCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGATTGACAACGTGGGGAAGCAGTTCGGAAACAGTTGTATTCATGATTCCGCTTGAGGGATTTTGAATCTGGAGTGGCTTATTGAGCTGTTCtttt  >  1:200447/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           CCGCCGATGGCGATGAGCTGGATATGCCTGTTTTTCAAGCCTCTCTGCAACGTTTGCTGATTTATATTGTCTTTCGTCATGTCGTCTGTCACTTTATGTGTTCCTCCATTGAAAATTGTTTATTGAGTCAGCAATCTATTTTTAATGATTATTATAACACTAAAATTTCTATCTTTTTTACTAAAAAAGGATTGATGAAAAGAATTTTAATGTGATTTGGACTATTTCCCGAAAAA‑GCGGCTCCATTTAGGGAGCCGCTTTTTTATGCTTTTTCAGTTTTTGTCATAAACAGGTAGAGCCCGTAATCTGTCGGTGCGGATGCGTAGCCTGCCTGCCGCAGCATTGCTGTGATATTGCCGCGGTGGTATGTCCCGTGATTGACAACGTGGGGAAGCAGTTCGGAAACAGTTGTTTTCATGATTCCGCTTGAGGGATTTTGAATCTGGAGTGGCTTATTGAGCTGTTCTTTT  >  NC_000964/607984‑608453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑