Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	166,037	1	.	T	100.0%	100.4 / NA	28	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/16);  total (12/16)

TCGGTCGTAGGTTCGAATCCTACCTGCGGAGC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCT  >  NC_000964/165795‑166264                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    tCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAAc                                                                                                                                                                                                                               >  1:212797/1‑251 (MQ=255)   ggTCGAAGGTTCGAATCCTACCTACGGTGCCATTGCTTCCATAGCTCATCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGGATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAAGCCCGTCTGCCGCTCCATTTATCCTAACgg                                                                                                                                                                                                                             <  2:166912/251‑1 (MQ=38)                aaTCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaatt                                                                                                                                                                                                                <  1:274259/251‑1 (MQ=255)                aaTCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGAACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGATTGGAAGCTTAAATGTATTATTACCAAGGATTATCCTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGCAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATCTTGTAaatt                                                                                                                                                                                                                >  2:262524/1‑251 (MQ=38)                 aTCCTCCCTGCGGAGCCATTGCCTCCATAGCTCAGCAGGTAGAGCTCTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCTCGGGTTCGAATCCCTTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaattg                                                                                                                                                                                                               <  1:261345/251‑1 (MQ=255)                              gcc‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                                                                                                                                 <  1:284918/249‑1 (MQ=255)                                     gCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGAAGCTCAAt                                                                                                                                                                                           <  1:20624/251‑1 (MQ=255)                                      cTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAtt                                                                                                                                                                                          >  1:18654/1‑251 (MQ=255)                                          cATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGAt                                                                                                                                                                                      <  2:53767/251‑1 (MQ=255)                                            tAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGAt                                                                                                                                                                                      >  1:156457/1‑249 (MQ=255)                                             aGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGAt                                                                                                                                                                                      >  1:273132/1‑248 (MQ=255)                                             aGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATag                                                                                                                                                                                    <  2:239948/250‑1 (MQ=255)                                                                       gTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTa                                                                                                                                                         <  2:40683/251‑1 (MQ=255)                                                                                  gTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATAATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGGCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATGTTGAAAATTGAATTGCTCCCGTAGCTCAATTGGATAGCGCGTTTGACTACGGATCAAAAGGTTAGAGGTTCGAct                                                                                                                                              >  2:136243/1‑251 (MQ=38)                                                                                                 ccGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAACGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGcc                                                                                                                               <  2:303822/251‑1 (MQ=255)                                                                                                        gAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtt                                                                                                                        >  1:347055/1‑251 (MQ=255)                                                                                                                                      tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTACTAGATTAGACAGGAAATCGGGAAGt                                                                                           >  2:154951/1‑250 (MQ=255)                                                                                                                                       cATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAg                                                                                            <  2:40218/248‑1 (MQ=255)                                                                                                                                        aTAAGGAGAACGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTACATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGc                                                                                        >  1:37647/1‑251 (MQ=255)                                                                                                                                                aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAAAACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTg                                                                                <  2:212797/251‑1 (MQ=255)                                                                                                                                                                             tagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGt                                                   >  1:62241/1‑251 (MQ=255)                                                                                                                                                                                 tCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGc                                                <  1:173709/250‑1 (MQ=255)                                                                                                                                                                                                  ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCtt                              <  1:247887/251‑1 (MQ=255)                                                                                                                                                                                                            gTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCAtt                    >  1:97571/1‑251 (MQ=255)                                                                                                                                                                                                               ggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCAttttt                 <  2:126795/251‑1 (MQ=255)                                                                                                                                                                                                                         gTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGAcc                       <  1:58584/235‑1 (MQ=255)                                                                                                                                                                                                                         gTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGAcc                       >  2:58584/1‑235 (MQ=255)                                                                                                                                                                                                                              aattccGTCTTCCGCTCCATTTATCATAACGGGATATTGTAAAGTGAATGGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGACATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCt  <  2:18654/247‑1 (MQ=14)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    TCGGTCGTAGGTTCGAATCCTACCTGCGGAGC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCT  >  NC_000964/165795‑166264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑