Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	2,097,087	(A)7→8	intergenic (‑111/+39)	azoJ ← / ← yocK	FMN‑dependent NADH‑azoreductase/putative general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	2,097,087	1	.	A	100.0%	49.2 / NA	15	intergenic (‑111/+39)	azoJ/yocK	FMN‑dependent NADH‑azoreductase/putative general stress protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/4);  total (11/4)

AATTCAACCACTTCATCATTAGGGTTGTTTTCTTTATAAGCAGCTAAGAAAGCTTCGTAAAGTTTAGTTGAAACGCCTTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAA‑‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACTGTCATTCTTGCATAAGGAACAGCTTCAAGTCTTTCGTAAGGAATC  >  NC_000964/2096852‑2097333                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                        aaTTCAACCACTTCATCATTAGGGTTGTTTTCTTTATAAGCAGCTAAGAAAGCTTCGTAAAGTTTAGTTGAAACGCCTTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACa                                                                                                                                                                                                                                               <  1:46837/246‑1 (MQ=255)          aCTTCATCATTAGGGTTGTTTTCTTTATAAGCAGCTAAGAAAGCTTCGTAAAGTTTAGTTGAAACGCCTTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGgtgt                                                                                                                                                                                                                                 >  2:137575/1‑251 (MQ=255)             tcatcaTTAGGGTTGTTTTCTTTATAAGCAGCTAAGAAATCTTCGTAAAGTTTAGTTGAAACGCCTTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATAGCGGTGTa                                                                                                                                                                                                                                <  1:318661/249‑1 (MQ=255)                                       agcagaTAAGGAACCTTCGTAAGGATTAGTTGAAACGCCTTCTTCAGCTCAACGGTCGCTTGCTATTACAAATAAACCTGAAGACATTTGTATTCCTCCCGGTTAACTTTTTATATATACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAGTATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGAATTTTTACTTCCAATCtt                                                                                                                                                                                                    >  2:284483/1‑251 (MQ=255)                                              aaGAAAGCTTCGTAAAGTTTAGTTGAAACGCATTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACg                                                                                                                                                                                             >  2:234896/1‑251 (MQ=255)                                                                                      cTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACACTTGAATTCCTCCCGGTAAACTTTTTATATTTACTTCCAAATAGTCTGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GAAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAAtt                                                                                                                                                     >  1:287177/1‑251 (MQ=255)                                                                                                       tttACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAAGCAAGTACATCTAATATCGGTGTATTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGAt                                                                                                                                      <  1:245077/250‑1 (MQ=255)                                                                                                                 aaaCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTAcc                                                                                                                          >  1:327248/1‑251 (MQ=255)                                                                                                                   aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTg                                                                                                                        >  2:143325/1‑251 (MQ=255)                                                                                                                                            gTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATaaga                                                                                                >  1:197736/1‑250 (MQ=255)                                                                                                                                                                                        tttACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTc                                                   >  1:263843/1‑251 (MQ=255)                                                                                                                                                                                            cGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACt                                               <  1:143325/251‑1 (MQ=255)                                                                                                                                                                                                aaGTCCATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACTGTc                                            >  2:105264/1‑250 (MQ=255)                                                                                                                                                                                                             tATTTTGAATATGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACTGTCATTCTTGCATAAgg                              >  2:173942/1‑251 (MQ=255)                                                                                                                                                                                                                      tatGAGATTTTTTTTCAAAAAAAA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACTGTCATTCTTGCATAAGGAACAGCTTc                     >  1:101282/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaaaa‑‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACTGTCATTCTTGCATAAGGAACAGCTTCAAGTCTTTCGTAAGGAAtc  >  2:185183/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                        AATTCAACCACTTCATCATTAGGGTTGTTTTCTTTATAAGCAGCTAAGAAAGCTTCGTAAAGTTTAGTTGAAACGCCTTCTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAA‑‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAACTCTCTTTCATAAGAAGGAGCATCTGTCTCCAGATCATCCTCCACATCAGCTTGGGCCTCAACTGTCATTCTTGCATAAGGAACAGCTTCAAGTCTTTCGTAAGGAATC  >  NC_000964/2096852‑2097333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑