Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	1,194,908	(T)8→9	intergenic (+81/‑126)	yitZ → / → argC	putative transport protein/N‑acetylglutamate gamma‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	1,194,908	1	.	T	100.0%	77.4 / NA	21	intergenic (+81/‑126)	yitZ/argC	putative transport protein/N‑acetylglutamate gamma‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/9);  total (12/9)

CGTGGAAAGAAGATCGGGAATTACCATTTTTGGACAGCTGTCTTTTCAGCAGCAGCCATTATGCTCGGGGGCTTTCTCGCTGACTTTTTCACAGTGCAAATGATTTTTTATGCAAGCTCTATTCTATATTTTTTGAGTGGATTGATGATGATGAAAACAGGCTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTT‑GAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTC  >  NC_000964/1194663‑1195153                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ggttttcatagctttcattatagtcctcgtcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTgctgatttttttttgagc                                                                                                                                                                                                                                                   >  1:6687‑M1/163‑246 (MQ=255)      tcatagctttcattatagtcctcgtcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAAt                                                                                                                                                                                                                                              <  1:123721‑M1/94‑1 (MQ=255)               tcattatagtcctcgtcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTg                                                                                                                                                                                                                                      >  2:62011‑M1/149‑250 (MQ=255)                   tatagtcctcgtcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcgtatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCACCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACa                                                                                                                                                                                                                                  >  2:300751‑M1/145‑250 (MQ=255)                      agtcctcgtcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGGCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGaa                                                                                                                                                                                                                               >  2:48082‑M1/142‑250 (MQ=255)                          cgcgtcctcaatgtaaagcctctcagcaaacccgtattatgaggatttatgtaagcgaatgaaacagcatcccggaaaggcttgcggatgaatgatttttggagctcaaggcgcgagttccgggaccggaaatcgatGGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTGTGTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAAttt                                                                                                                                                                                                                          >  1:74441‑M1/138‑251 (MQ=255)                                                                           tttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCAt                                                                                                                                                                         <  1:270466‑M1/163‑1 (MQ=255)                                                                                  gaatgaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTtata                                                                                                                                                                   <  1:247568‑M1/169‑1 (MQ=255)                                                                                      gaaacagcatccctgcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGt                                                                                                                                                               >  2:326596‑M1/78‑250 (MQ=255)                                                                                                    gcaaggcttgcggatgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTcaca                                                                                                                                                 >  1:145846‑M1/64‑250 (MQ=255)                                                                                                                  tgaatgattttttgagctcaaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACAAAGAGGGTg                                                                                                                                  <  2:144225‑M1/202‑1 (MQ=255)                                                                                                                       gatttttttagctcaaggttcgagttccggtaccggaaatcgatTGACTCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACt                                                                                                                              >  2:58886‑M1/45‑250 (MQ=255)                                                                                                                                     aaggctcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACTGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAgg                                                                                                               <  2:340400‑M1/221‑1 (MQ=255)                                                                                                                                          tcgagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAACTGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGCTTTTTTTTTGAGCGGAATTCCCATTGACCAGAAAATTTATAAATGAATAAAAATATTCAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCACGAGGGAGAACTTGTTTTACAAATAGGAATTg                                                                                                          <  2:243715‑M1/226‑1 (MQ=255)                                                                                                                                            gagttccggtaccggaaatcgatTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATGCCCATTGAACAGAAAATTTATAAATGAATAAAAATGTTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAAGTGTa                                                                                                        >  1:92859‑M1/24‑251 (MQ=255)                                                                                                                                                                    gACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCAcc                                                                                >  1:239094/1‑251 (MQ=255)                                                                                                                                                                      cgcgGTCAGCATGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTATATATACGATTGAATTAATTTTTTTTCATGTTATAATGTTAAATAATTTCACAAACACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCg                                                                               >  2:229864/1‑250 (MQ=255)                                                                                                                                                                            cAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGt                                                                        >  2:69388/1‑251 (MQ=255)                                                                                                                                                                                              ggCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGcatc                                                       >  1:135898/1‑250 (MQ=255)                                                                                                                                                                                                                       gTTTTTGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACtt                             <  2:135898/251‑1 (MQ=255)                                                                                                                                                                                                                          tttGTGCTGGAGTCGTGCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACTATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTa                           <  1:193710/250‑1 (MQ=255)                                                                                                                                                                                                                                          gCTGATTTTTTTTTGAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAg          <  1:69388/251‑1 (MQ=255)                                                                                                                                                                                                                                            gattttttttt‑GAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAgg         <  1:528/246‑1 (MQ=255)                                                                                                                                                                                                                                            gattttttttt‑GAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGa       <  2:239094/248‑1 (MQ=255)                                                                                                                                                                                                                                             attttttttt‑GAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGaa      <  1:313888/249‑1 (MQ=255)                                                                                                                                                                                                                                                  ttttt‑GAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTc  <  1:340400/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CGTGGAAAGAAGATCGGGAATTACCATTTTTGGACAGCTGTCTTTTCAGCAGCAGCCATTATGCTCGGGGGCTTTCTCGCTGACTTTTTCACAGTGCAAATGATTTTTTATGCAAGCTCTATTCTATATTTTTTGAGTGGATTGATGATGATGAAAACAGGCTGACGCGGTCAGCCTGTTTTTTTATGCGGCTGAAATGCGGCACCGCATCAACGTTTTTGTGCTGGAGTCGTGCTGATTTTTTTT‑GAGCGGAATTCCCATTGAACAGAAAATTTATAAATGAATAAAAATATTAAATATACGATTGAATTAATTTTTATTCATGTTATAATGTTAAATAATTTCACAAAGACCAAGAGGGTGAACTTGTTTTGAAAATAGGAATTGTAGGTGCTACAGGATATGGAGGCACCGAACTTGTCAGGATTCTTTCGCATCATCCTCATGCAGAGGAATGCATACTTTATTCATCCAGCGGAGAAGGGAATGTC  >  NC_000964/1194663‑1195153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑