Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	166,345	Δ1 bp	intergenic (+17/‑155)	trnI‑Ala → / → rrnH‑16S	tRNA‑Ala/ribosomal RNA‑16S

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	166,345	0	G	.	97.4%	78.9 / ‑2.7	39	intergenic (+17/‑155)	trnI‑Ala/rrnH‑16S	tRNA‑Ala/ribosomal RNA‑16S
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (23/15);  total (24/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATG  >  NC_000964/166104‑166576                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        cAAAAGGTTAGGGGTTCGACTCCGCTCGGGCGTGTCATGTCTTTTAATAGAATAGATAGGAAATCGGGCTGGAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTGCGAATCCTGTCTTCCCGGCCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAAGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACtt                                                                                                                                                                                                                               <  2:152068/251‑1 (MQ=11)     aGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTga                                                                                                                                                                                                                            >  1:275134/1‑250 (MQ=14)      ggTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTga                                                                                                                                                                                                                            >  1:305182/1‑249 (MQ=14)          aGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGt                                                                                                                                                                                                                      >  2:130546/1‑251 (MQ=14)                 cGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTg                                                                                                                                                                                                               >  1:81124/1‑251 (MQ=18)                                              aatagaatagATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCg                                                                                                                                                                                  >  2:241831/1‑251 (MQ=18)                                                      agatagGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaac                                                                                                                                                                           >  1:116591/1‑250 (MQ=18)                                                          agGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAAt                                                                                                                                                                      >  1:130973/1‑251 (MQ=18)                                                                 cGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCttt                                                                                                                                                               <  2:156457/251‑1 (MQ=18)                                                                 cGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCttt                                                                                                                                                               >  1:155485/1‑251 (MQ=18)                                                                     aaGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaa                                                                                                                                                            >  2:48194/1‑250 (MQ=18)                                                                              aGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGACTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACa                                                                                                                                                   >  2:324150/1‑250 (MQ=18)                                                                                        agCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGt                                                                                                                                        >  1:159938/1‑251 (MQ=18)                                                                                             aTGGTTTGGGACCATGGGGTCGCAGGTTTGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg                                                                                                                                   >  2:80984/1‑251 (MQ=18)                                                                                                   tGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACta                                                                                                                                                                                                        <  1:170398/176‑1 (MQ=21)                                                                                                   tGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACta                                                                                                                                                                                                        >  2:170398/1‑176 (MQ=21)                                                                                                   tGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAt                                                                                                                              <  1:130546/250‑1 (MQ=18)                                                                                                   tGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAt                                                                                                                              <  1:86778/250‑1 (MQ=18)                                                                                                        ccctGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCaatt                                                                                                                        >  2:46201/4‑248 (MQ=18)                                                                                                           tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGttttt                                                                                                                     >  1:241576/1‑251 (MQ=18)                                                                                                            ggggTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGatttt                                                                                                                     >  2:11406/1‑250 (MQ=18)                                                                                                                         cGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAAGCGCACAg                                                                                                        <  2:97571/250‑1 (MQ=18)                                                                                                                                     tCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTagt                                                                                           >  1:161152/1‑251 (MQ=18)                                                                                                                                       ccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCACAAGTTTTTAAAAAAG‑TTATTGACTTTGACGAAGTGACATTTTATCCTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGTACTTTGAAAACTAACCAAGACAAACCGAACCTGTTAATTCAGTTTTTAAAAATCGAAAAGAGATGGGCGTagtc                                                                                          >  1:188431/1‑250 (MQ=11)                                                                                                                                               tttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAac                                                                                  <  1:154951/250‑1 (MQ=14)                                                                                                                                                    aTGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACttt                                                                            <  2:81124/251‑1 (MQ=14)                                                                                                                                                      ggggCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTTCTTTAt                                                                          <  2:273132/251‑1 (MQ=14)                                                                                                                                                                       gggAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTg                                                         <  2:130973/251‑1 (MQ=14)                                                                                                                                                                       gggAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAA‑CGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTg                                                         >  2:318624/1‑251 (MQ=14)                                                                                                                                                                          agagCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGAGCCTGGCt                                                      <  2:62241/251‑1 (MQ=14)                                                                                                                                                                             gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTc                                                     >  1:211265/1‑249 (MQ=14)                                                                                                                                                                                         cACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTgg                                       <  2:275134/251‑1 (MQ=14)                                                                                                                                                                                                gaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTCAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGACGGATGGCGGCGTg                                >  2:294957/1‑251 (MQ=9)                                                                                                                                                                                                 aggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGc                               >  1:337168/1‑251 (MQ=14)                                                                                                                                                                                                      aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGGTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCACACTACTTTATCGTAGTGTTTGATCCTGGCTCAGGACGAAAGCTGGCGGCGTGCCTAAt                          <  2:25757/251‑1 (MQ=255)                                                                                                                                                                                                         ggTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATAc                        >  1:317945/1‑250 (MQ=14)                                                                                                                                                                                                                        ggCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGa        <  2:161152/251‑1 (MQ=14)                                                                                                                                                                                                                         gCTCCACCAAAAGTTTTTAAAAAAG‑TTATTGGCTTTGAAGAAGTGACATTGTATACTAATAAAGGTGCTTTAACAAAGCGGACAAACAACATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTCTTTAAAAATCGCACAGCTATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGaa       <  2:37647/251‑2 (MQ=11)                                                                                                                                                                                                                              accaAAAGTTTTTAAAAAAG‑TTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATg  >  1:234521/1‑251 (MQ=14)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        CAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTCAGCTTGGTAGAGCACATGGTTTGGGACCATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTAAAAATCGCACAGCGATGTGCGTAGTCAGTCAAACTACTTTATCGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATG  >  NC_000964/166104‑166576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑