Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	2,480,667	Δ1 bp	intergenic (+42/+83)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	2,480,667	0	T	.	100.0%	107.3 / NA	24	intergenic (+42/+83)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/9);  total (15/9)

AATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCTTGAGAATGATGGCACCGG  >  NC_000964/2480424‑2480908                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  aaTTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTa                                                                                                                                                                                                                                           <  1:290223/250‑1 (MQ=255)     cACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTg                                                                                                                                                                                                                                      >  2:105595/1‑251 (MQ=255)       cGACTGTCTTCTTGGCGATGCAACCAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGa                                                                                                                                                                                                                                     >  1:78821/1‑250 (MQ=255)                      cGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                                                                                                                                     >  2:20434/1‑251 (MQ=255)                       gATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTAGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                                                                                                                                    >  1:185081/1‑251 (MQ=255)                                aaCTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaata                                                                                                                                                                                                           <  2:268378/251‑1 (MQ=255)                                   tttGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAAc                                                                                                                                                                                                         >  1:8131/1‑250 (MQ=255)                                                       tGCCCTTTCTTTGAGCTTTGTCGTCTCGATTTCTGAAACCTGGGCAGCAAACAAAACCCTAACCCCTAACCACGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTTGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTTC‑AAGCATTTTCGG‑CTTTTTTACGCCGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAaga                                                                                                                                                                                     <  1:262834/250‑1 (MQ=255)                                                          ccTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                                                                                                                                           <  1:147802/209‑1 (MQ=255)                                                          ccTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                                                                                                                                           >  2:147802/1‑209 (MQ=255)                                                                  ggAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGtt                                                                                                                                                                         >  1:151752/1‑251 (MQ=255)                                                                                   acttCTGAAACAAGGGCAACAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATTGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCCTACACTGGGGGAACATATAATAAGAAAAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAATAACTTCGGGGGGTTCTATTGATTACTTc                                                                                                                                                          <  1:247417/247‑1 (MQ=255)                                                                                         gAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                                                                                                                                   <  1:343289/250‑1 (MQ=255)                                                                                                             aaCCTTATCTCCTAACTACGAAGCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGGTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCcttctt                                                                                                                              >  1:38283/1‑251 (MQ=255)                                                                                                                     ctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGt                                                                                                                       >  1:266245/1‑250 (MQ=255)                                                                                                                        cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGc                                                                                                                   >  2:202170/1‑251 (MQ=255)                                                                                                                           aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTc                                                                                                                 >  1:218225/1‑250 (MQ=255)                                                                                                                                            aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCaaa                                                                                               <  1:100556/251‑1 (MQ=255)                                                                                                                                               ggACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAagt                                                                                             >  1:276480/1‑250 (MQ=255)                                                                                                                                                                                       tACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGc                                                     <  1:183265/250‑1 (MQ=255)                                                                                                                                                                                              gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGt                                             >  1:18925/1‑251 (MQ=255)                                                                                                                                                                                                                  aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAAt                         <  1:196420/251‑1 (MQ=255)                                                                                                                                                                                                                        gCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGAATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCtt                   >  2:289019/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCTTGAGAATGATGGCACCgg  >  1:173882/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  AATTCACGACTGTCTTCTTGGCGATGCAACAAACTTTGCACACTGGGATGAAGTTGCCCTTTCTTGGAGCTTTGTCGACTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACGCTCATAAGTATGAGCGCCAAAGTAGTCGCGCTGTGCTTGGATTAAGTTTGCAGGCAGTACGGCTGTGCGGTAGCTGTCATAGTAGGCTAATGCGCTTGAGAATGATGGCACCGG  >  NC_000964/2480424‑2480908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑