Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000964	165,751	Δ1 bp	intergenic (+44/‑3)	rrnI‑5S → / → trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000964	165,751	0	C	.	100.0%	220.5 / NA	49	intergenic (+44/‑3)	rrnI‑5S/trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/29);  total (20/29)

AATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGC‑A‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTT  >  NC_000964/165514‑165990                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  aaTCGATCGAGGTCTTAACCATATTGTTGAATGATTTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCGCACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTAGCATGGTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                                                                                                                                                    <  2:171908/251‑1 (MQ=14) aaTCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGc                                                                                                                                                                                                                                      <  1:118143/249‑1 (MQ=255)        cgaGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                                                                                                                                             <  1:192200/251‑1 (MQ=255)             aCTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCt                                                                                                                                                                                                                        <  1:272834/251‑1 (MQ=255)                           ttGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                                                                                                                                                          >  2:247887/1‑251 (MQ=255)                                tgatgTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAatcgatcg                                                                                                                                                                                                     >  2:188781/1‑251 (MQ=255)                                tgatgTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCg                                                                                                                                                                                                     >  1:126795/1‑251 (MQ=255)                                 gatgTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCgg                                                                                                                                                                                                    >  1:265547/1‑251 (MQ=255)                                             gTTAGCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAGCGGCTGTTACCCGATCGGTCGTAGGt                                                                                                                                                                                            <  1:103812/247‑1 (MQ=255)                                               tATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCTGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTAGGTTCGAAt                                                                                                                                                                                      >  1:303822/1‑251 (MQ=255)                                               tATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGaa                                                                                                                                                                                       <  2:269997/250‑1 (MQ=255)                                               tATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAAt                                                                                                                                                                                      >  1:315332/1‑251 (MQ=255)                                                   tAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCt                                                                                                                                                                                   >  1:40683/1‑250 (MQ=255)                                                     gTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATTGGTCGTAGGTTCGAATCCTAcc                                                                                                                                                                                >  2:141892/1‑251 (MQ=255)                                                       tttGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCTTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATGCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCt                                                                                                                                                                               <  2:262431/250‑1 (MQ=255)                                                            gagaACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACCCCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCTCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCTGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAg                                                                                                                                                                         <  2:322694/251‑1 (MQ=255)                                                                acacTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGccat                                                                                                                                                                     <  2:322001/251‑4 (MQ=255)                                                                 cacTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGccatt                                                                                                                                                                    >  1:239948/1‑247 (MQ=255)                                                                       cAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGGGCCA‑TTGCtt                                                                                                                                                               <  2:170982/249‑1 (MQ=255)                                                                                 ggtggCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTcagc                                                                                                                                                   >  1:226097/1‑251 (MQ=255)                                                                                    ggCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAgg                                                                                                                                                <  1:75642/251‑1 (MQ=255)                                                                                                 agGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                                                   <  1:321115/251‑1 (MQ=255)                                                                                                      acacCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGt                                                                                                                               >  1:138094/1‑250 (MQ=255)                                                                                                        acCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg                                                                                                                            <  2:69648/251‑1 (MQ=255)                                                                                                           cGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                                                         <  1:338043/251‑1 (MQ=255)                                                                                                             ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGa                                                                                                                          >  1:53767/1‑248 (MQ=255)                                                                                                                         acacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTGCCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTc                                                                                                           <  2:273455/251‑1 (MQ=255)                                                                                                                                     aGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                                                               <  2:265547/251‑1 (MQ=255)                                                                                                                                                   gATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGTTCTGTAGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCAAAGCTCAGCAGGTCGAGCACTTCCCTTGAAAGGAAGAGGTCATCGGTTCGAGCCCGATTGGAAGCTTAAATggat                                                                                 >  2:173709/1‑248 (MQ=37)                                                                                                                                                      ggTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtatta                                                                               <  1:238829/250‑1 (MQ=255)                                                                                                                                                            cGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCaa                                                                        <  2:203643/251‑1 (MQ=255)                                                                                                                                                             gggggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAg                                                                       <  1:82499/251‑1 (MQ=255)                                                                                                                                                                 gTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGttt                                                                   >  1:40218/1‑251 (MQ=255)                                                                                                                                                                           gtgAGAGTAGGACGCCTCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑AGTCCGCAGTAGCTCAGGGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAgg                                                          <  2:20455/250‑1 (MQ=38)                                                                                                                                                                                 gTAGGACGCCGCCAAGCAGATTTATTAATTCTTCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGc                                                   <  2:140253/251‑1 (MQ=255)                                                                                                                                                                                 gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGc                                                   <  1:123443/251‑1 (MQ=255)                                                                                                                                                                                    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttt                                                <  1:124970/251‑1 (MQ=255)                                                                                                                                                                                     gACGCCGCCCAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTAGTAGGTTCGAATCCTACCTGCGGAGC‑CCTTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCtttt                                               >  2:274259/1‑251 (MQ=38)                                                                                                                                                                                        gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGTAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttttt                                            <  1:284614/251‑1 (MQ=255)                                                                                                                                                                                              aaGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg                                      >  1:152068/1‑251 (MQ=255)                                                                                                                                                                                               aGCAGATTTATTAATTCTTCCATGTTTTTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTTCCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAATATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGa                                     <  1:176034/251‑1 (MQ=38)                                                                                                                                                                                                      ttattaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGGAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTAGGAAGCATAAATGTATTATTACAAAAGTTTCTCTTAAGGAGAAAGATTTTTTTATTGCGATATACgg                              >  2:22828/1‑251 (MQ=37)                                                                                                                                                                                                                              aTGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGt                                                                                                                    >  2:149286/1‑141 (MQ=21)                                                                                                                                                                                                                              aTGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGt                                                                                                                    <  1:149286/141‑1 (MQ=21)                                                                                                                                                                                                                              aTGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttt                                                >  2:170523/1‑209 (MQ=255)                                                                                                                                                                                                                              aTGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttt                                                <  1:170523/209‑1 (MQ=255)                                                                                                                                                                                                                                 aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                                                        <  1:18887/166‑1 (MQ=255)                                                                                                                                                                                                                                 aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                                                        >  2:18887/1‑166 (MQ=255)                                                                                                                                                                                                                                  aGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCA‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACTCCACCtt  <  2:315332/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  AATCGATCGAGGACTTAACCATATTTTTGAATGATGTCACATCTGTTATCTAGTTTTGAGAGAACACTCTCAATTTGTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGC‑A‑TTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTT  >  NC_000964/165514‑165990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑