breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 30,389 T→G noncoding (111/1554 nt) rrnA‑16S → ribosomal RNA‑16S
RA 165,749 Δ1 bp intergenic (+42/‑5) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn
RA 165,751 Δ1 bp intergenic (+44/‑3) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn
RA 165,826 +C intergenic (+1/‑4) trnI‑Asn → / → trnI‑Thr tRNA‑Asn/tRNA‑Thr
RA 166,037 +T intergenic (+4/‑27) trnI‑Gly → / → trnI‑Arg tRNA‑Gly/tRNA‑Arg
RA 166,345 Δ1 bp intergenic (+17/‑155) trnI‑Ala → / → rrnH‑16S tRNA‑Ala/ribosomal RNA‑16S
RA 201,889 A→G N538S (AAC→AGC)  glmS → L‑glutamine‑D‑fructose‑6‑phosphate amidotransferase
JC 268,678 (CCGGT)1→2 coding (789/927 nt) kdgD → 5‑dehydro‑4‑deoxyglucarate dehydratase
RA 454,634 A→G intergenic (+42/+18) sipU → / ← yczH type I signal peptidase/putative hydrolase
RA 652,828 A→G intergenic (+960/‑604) groEL → / → ydiM chaperonin large subunit/hypothetical protein; prophage 3 region
MC JC 653,283 Δ11,391 bp ydiM–ydjC ydiM, ydzU, bsuMA, bsuMB, bsuRA, bsuRB, bsuRC, ydjB, ydjC
RA 774,021 A→G intergenic (+41/‑117) rhgX → / → yesY rhamnogalacturonan exolyase/rhamnogalacturonan acetylesterase
RA 785,980 G→A intergenic (‑75/‑1735) yetH ← / → yezD putative lyase/dioxygenase/hypothetical protein
RA 1,147,714 T→C V989A (GTT→GCT)  sbcC → DNA ATP‑dependent repair enzyme
RA 1,174,107 +G intergenic (+16/+29) slpS → / ← yitE 2‑phospho‑3‑sulfolactate synthase/putative integral inner membrane protein
RA 1,174,312 T→C I152V (ATC→GTC)  yitE ← putative integral inner membrane protein
RA 1,263,383 A→G intergenic (+522/+319) trnSL‑Val2 → / ← yjcM tRNA‑Val/conserved hypothetical protein; phage island
RA 1,263,447 A→G intergenic (+586/+255) trnSL‑Val2 → / ← yjcM tRNA‑Val/conserved hypothetical protein; phage island
RA 1,263,647 T→C intergenic (+786/+55) trnSL‑Val2 → / ← yjcM tRNA‑Val/conserved hypothetical protein; phage island
RA 1,279,570 A→G C312R (TGC→CGC)  ctaO ← minor protoheme IX farnesyltransferase 1 (heme O synthase)
RA 1,279,662 C→T G281D (GGC→GAC)  ctaO ← minor protoheme IX farnesyltransferase 1 (heme O synthase)
RA 1,279,841 T→C K221K (AAA→AAG)  ctaO ← minor protoheme IX farnesyltransferase 1 (heme O synthase)
RA 1,280,111 T→C G131G (GGA→GGG)  ctaO ← minor protoheme IX farnesyltransferase 1 (heme O synthase)
RA 1,317,152 +GT intergenic (+23/+5) phrA → / ← yjpA secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
RA 1,317,158 (T)5→6 coding (257/258 nt) yjpA ← putative enzyme
MC JC 1,320,630 Δ27,517 bp [xkdA]–[xlyA] 38 genes
RA 1,708,027 G→T G105V (GGA→GTA)  flhA → component of the flagellar export machinery
RA 1,781,832 T→C intergenic (‑47/‑74) ymcC ← / → pksA putative integral inner membrane protein/transcriptional regulator of bacillaene synthesis operon
RA 1,879,987 A→G intergenic (+228/‑100) glnA → / → ynxB glutamine synthetase/putative phage protein; defective, phage region
MC JC 1,880,060 20479 bp→TCTC ynxB–dutA 21 genes
RA 2,045,799 T→A I10I (ATA→ATT)  yoaU ← putative transcriptional regulator (LysR family)
RA 2,046,458 T→C I177T (ATT→ACT)  yoaV → putative modified amino acid or TCA compound efflux permease
RA 2,097,087 (A)7→8 intergenic (‑111/+39) azoJ ← / ← yocK FMN‑dependent NADH‑azoreductase/putative general stress protein
RA 2,145,201 A→G S164P (TCA→CCA)  yodT ← putative aminovalerate aminotransferase
RA 2,145,413 C→T W93* (TGG→TAG)  yodT ← putative aminovalerate aminotransferase
RA 2,145,776 A→G F17F (TTT→TTC)  BSU_19745 ← hypothetical protein
RA 2,292,293 T→C intergenic (‑9/+139) scuA ← / ← ypmP assembly factor BSco of the Cu(A) site of cytochrome c oxidase/hypothetical protein
RA 2,293,417 A→C P207P (CCT→CCG)  ilvA ← threonine dehydratase
RA 2,480,646 2 bp→AT intergenic (+21/+103) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,480,654 Δ1 bp intergenic (+29/+96) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,480,667 Δ1 bp intergenic (+42/+83) zwf → / ← gndA glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
RA 2,527,177 G→C Q364E (CAG→GAG)  xseA ← exodeoxyribonuclease VII (large subunit)
RA 2,532,497 A→G L171L (CTT→CTC)  spoIIIAH ← stage III sporulation ratchet engulfment protein
RA 2,536,709 G→A T302M (ACG→ATG)  spoIIIAA ← ATP‑binding stage III sporulation protein
RA 2,581,732 (T)6→7 intergenic (‑115/+39) pstS ← / ← pbpA phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A)
RA 2,626,956 G→A A331V (GCG→GTG)  dnaK ← molecular chaperone, ATP‑dependent
RA 2,750,375 T→C V239A (GTT→GCT)  yraK → putative hydrolase
RA 2,750,754 T→A intergenic (+279/+129) yraK → / ← yraJ putative hydrolase/hypothetical protein
RA 2,751,171 C→T Q23Q (CAG→CAA)  yraJ ← hypothetical protein
RA 2,751,293 T→C *120W (TAG→TGG)  yraI ← hypothetical protein
RA 3,400,383 A→T intergenic (‑23/+154) yvqJ ← / ← yvqK putative efflux protein/putative ATP:cob(I)alamin adenosyltransferase
RA 3,770,066 (A)8→9 intergenic (‑613/+38) ywzE ← / ← csbD conserved protein of unknown function (putative phage interference protein)/stress response protein
RA 3,835,994 A→G intergenic (‑249/‑64) ywiB ← / → sboA putative RNA binding protein/subtilosin A
RA 3,848,082 A→T I215N (ATT→AAT)  speB ← agmatinase
RA 3,935,825 Δ1 bp coding (1190/1191 nt) rlmI ← large subunit ribosomal RNA m5C1962 methyltransferase
RA 4,036,008 T→A L97F (TTA→TTT)  yxxE ← hypothetical protein
RA 4,047,888 T→A I117I (ATT→ATA)  hutM → histidine degradation metabolites permease
RA 4,048,106 T→A M190K (ATG→AAG)  hutM → histidine degradation metabolites permease
RA 4,048,425 A→G V296V (GTA→GTG)  hutM → histidine degradation metabolites permease
RA 4,155,395 (A)5→6 intergenic (‑186/+38) trnY‑Lys ← / ← purA tRNA‑Lys/adenylosuccinate synthetase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000964 161197–161234 163338–162902 1669–2142 2 [1] [1] 2 [rrnI‑16S]–[rrnI‑23S] [rrnI‑16S],[rrnI‑23S]
* * ÷ NC_000964 163589–164865 165474–164903 39–1886 2 [1] [1] 2 rrnI‑23S ribosomal RNA‑23S
* * ÷ NC_000964 166732 176125–172975 6244–9394 2 [1] [1] 2 [rrnH‑16S]–[rrnG‑23S] [rrnH‑16S],rrnH‑23S,rrnH‑5S,rrnG‑16S,[rrnG‑23S]
* * ÷ NC_000964 202128 213027 10900 16 [0] [0] 2 [ybbU]–[ybcL] [ybbU],alkA,adaA,adaB,ndhF,ybcC,ybcF,ybcH,ybcI,ybzH,[ybcL]
* * ÷ NC_000964 213235 220035 6801 2 [0] [0] 16 [ybcM]–skfH [ybcM],skfA,skfB,skfC,skfE,skfF,skfG,skfH
* * ÷ NC_000964 404458 406623 2166 17 [0] [1] 2 [ycxB]–[ycxD] [ycxB],ycxC,[ycxD]
* * ÷ NC_000964 406743 454595 47853 2 [1] [0] 17 [ycxD]–sipU [ycxD],yczE,tcyC,tcyB,tcyA,bsdA,ubiX,bsdC,bsdD,yclD,yclE,dtpT,yclG,yczF,gerKA,gerKC,gerKB,yclH,yclI,yclJ,yclK,rapC,phrC,yczM,yczN,thrD,pbtN,pbtO,pbtP,pbtQ,ycnB,ycnC,nfrAB,ycnE,yczG,gabR,gabT,gabD,glcU,gdh,cutI,cutJ,cutR,ycnL,mtlA,mtlF,mtlD,ycsA,sipU
* * ÷ NC_000964 529504 551448 21945 12 [0] [0] 2 ydcL–yddN ydcL,immA,immR,xis,ydzL,ydcO,BSU_04849,helP,conQ,nicK,ydcS,ydcT,yddA,conB,conC,conD,conE,yddF,conG,cwlT,yddI,yddJ,yddK,rapI,phrI,yddM,yddN
* * ÷ NC_000964 551611 587692 36082 2 [0] [1] 2 [lrpA]–ydzQ [lrpA],lrpB,yddQ,yddR,yddS,ydzM,yddT,ydzN,sufLC,cspC,cdnL,ydzE,ydeC,ydeD,ydeE,ydeF,ydeG,ydeH,ydeI,ydeJ,ydeK,ydeL,ydeM,ydeN,ydzF,ydeO,ydeP,ydeQ,ydeR,ydeS,ydzO,aseR,arsF,ydfB,ydfC,ydfD,ydfE,ydfF,ydfG,ydzP,ydzQ
* * ÷ NC_000964 587909 591949 4041 2 [1] [1] 2 [ydfH]–ydfJ [ydfH],ydfI,ydfJ
* * ÷ NC_000964 592151 596957 4807 2 [1] [1] 2 cesA–[ydfN] cesA,ydfK,ydfL,mneP,[ydfN]
* * ÷ NC_000964 597024 616193 19170 2 [1] [1] 2 [ydfN]–[ydhC] [ydfN],ydfO,ydfP,ydfQ,ydzH,ydzR,ydfR,ydfS,cotP,ydgA,ydgB,ydgC,ydgD,ydgE,vmlR,ydgF,dinB,ydgG,ydgH,ydgI,ydgJ,ydgK,ydhB,[ydhC]
* * ÷ NC_000964 616368 634383 18016 2 [1] [0] 12 [ydhC]–gmuG [ydhC],spoL,ydhE,ydhF,phoB,fra,ydhH,ydhI,ydhJ,ydhK,pbuE,gmuB,gmuA,gmuC,gmuD,gmuR,gmuE,gmuF,gmuG
* * ÷ NC_000964 753341 760010 6670 2 [1] [1] 2 [cotK]–[yesM] [cotK],yezE,yesE,yesF,cotJA,cotJB,cotJC,yesJ,yesK,yesL,[yesM]
* * ÷ NC_000964 760126 773980 13855 2 [1] [0] 28 [yesM]–[rhgX] [yesM],yesN,yesO,rhgP,rhgQ,rhgH,rhgR,rhgT,yesU,yesV,rhgW,[rhgX]
* * ÷ NC_000964 1148155 1160141 11987 15 [0] [1] 2 [hlpB]–[farP] [hlpB],gerPF,gerPE,gerPD,gerPC,gerPB,gerPA,pchI,yisJ,mblK,yisL,wprA,yisN,asnO,yizA,[farP]
* * ÷ NC_000964 1160236 1173162 12927 2 [1] [1] 2 [farP]–[slpH] [farP],yisQ,yisR,iolQ,iolX,yisT,yisU,yisV,yisX,yisY,yisZ,yitA,yitB,[slpH]
* * ÷ NC_000964 1173286 1173453 168 2 [1] [0] 2 [slpH]–[slpS] [slpH],[slpS]
* * ÷ NC_000964 1173609 1174095 487 2 [0] [0] 15 [slpS] [slpS]
* * ÷ NC_000964 1263668 1266650 2983 17 [0] [0] 2 yjcM–[yjcP] yjcM,yjcN,yjzF,yjzG,yjcO,[yjcP]
* * ÷ NC_000964 1266798 1269637 2840 2 [0] [1] 2 [yjcP]–yjdA [yjcP],yjcQ,yjcR,yjcS,yjdA
* * ÷ NC_000964 1269716 1270529 814 2 [1] [1] 2 yjdB yjdB
* * ÷ NC_000964 1270717 1279495 8779 2 [1] [0] 17 [manR]–yjdJ [manR],manP,manA,yjdF,yjdG,yjdH,trnE,yjzH,yjdJ
* * ÷ NC_000964 1782126 1791214 9089 8 [0] [1] 2 [pksA]–[pksH] [pksA],pksB,BSU_17099,pksC,pksD,pksE,acpK,pksF,pksG,[pksH]
* * ÷ NC_000964 1791377 1806658 15282 2 [1] [1] 3 [pksH]–[pksJ] [pksH],pksI,[pksJ]
* * ÷ NC_000964 1806791 1843831 37041 3 [1] [1] 2 [pksJ]–[pksN] [pksJ],pksL,pksM,[pksN]
* * ÷ NC_000964 1843877 1854450 10574 2 [1] [1] 2 [pksN]–[pksR] [pksN],[pksR]
* * ÷ NC_000964 1854554 1863809 9256 2 [1] [0] 8 [pksR]–[pghC] [pksR],pksS,ymzB,ymaE,aprX,[pghC]
* * ÷ NC_000964 1960197 1965391 5195 7 [0] [1] 3 ppsE–[ppsD] ppsE,[ppsD]
* * ÷ NC_000964 1965615 1974910 9296 3 [1] [1] 2 [ppsD]–[ppsC] [ppsD],[ppsC]
* * ÷ NC_000964 1975151 1981853 6703 2 [1] [0] 2 ppsC non‑ribosomal plipastatin synthetase C involved in synthesis of plipastatin
* * ÷ NC_000964 1981983 1988995 7013 2 [0] [1] 2 [ppsC]–[ppsB] [ppsC],[ppsB]
* * ÷ NC_000964 1989009 1997968 8960 2 [1] [0] 7 [ppsB]–ppsA [ppsB],ppsA
* * ÷ NC_000964 2046840 2063981 17142 13 [0] [0] 2 yoaW–phrK yoaW,yoaZ,penP,yobA,yobB,yppS,xynA,yozV,yobD,yozH,yozI,yobE,yobF,yozW,yozX,yozJ,yozY,rapK,phrK
* * ÷ NC_000964 2064145 2079058 14914 2 [0] [0] 13 yozL–yobO yozL,yozM,yobI,bsrE,yobJ,rttM,rttL,yobM,yobN,yobO
* * ÷ NC_000964 2146013 2169395 23383 11 [0] [1] 2 [yoyG]–[yosC] [yoyG],[cgeE],cgeD,cgeC,cgeA,cgeB,phyC,sprB,yotM,yotL,yotK,yotJ,yotI,yotH,yotG,yotF,yotE,BSU_19915,yotD,yotC,yotB,sspC,yosX,yosW,yosV,yojW,yosU,yosT,dutB,nrdFB,yosQ,nrdFB,nrdEB,nrdEB,nrdIB,yosL,yosK,yosJ,yosI,yosH,yosG,yosF,yosE,yosD,[yosC]
* * ÷ NC_000964 2169565 2292284 122720 2 [1] [1] 12 [yosB]–[scuA] [yosB],yosA,yorZ,yorY,yorX,yorW,yorV,mtbP,yorT,yorS,yorR,yorQ,yorP,yorO,yorN,yorM,dpoL,yorK,yorJ,yorI,yorH,yorG,yorF,yorE,yorD,yorC,yorB,yorA,pghZ,yoqY,yoqX,yoqW,yoqV,yoqU,yoqT,yoqS,yoqR,yoqP,yoqO,yoqN,yoqM,yoqL,yoqK,yoqJ,yoqI,yoqH,yoqG,yoqF,yoqE,yoqD,yoqC,yoqB,yoqA,yopZ,yopY,yopX,yopW,yopV,yopU,yopT,yopS,yopR,yopQ,yopP,yopO,yopN,yopM,aimX,aimP,aimR,yopJ,yopI,sieB,yopG,yopF,yopE,yoyH,yoyI,yopD,yopC,yopB,yopA,yonX,yonV,yonU,yoyJ,spbT,yonS,yonR,yonP,yonO,hupN,BSU_21058,yonK,yonJ,yonI,yonH,yonG,yonF,yonE,yonD,yonC,yonB,yonA,yomZ,yomY,yomX,yomW,yomV,yomU,youA,yomT,yomS,yomR,yomQ,yomP,yomO,yomN,yomM,yozP,yomL,youB,yomK,yomJ,cwlP,yomH,yomG,yomF,yomE,yomD,BSU_21409,blyA,bhlA,bhlB,bdbB,sunS,bdbA,sunT,sunA,sunI,uvrX,yolD,yolC,yolB,yolA,bsrG,yokL,yokK,yokJ,yokI,yokH,yokG,nukF,yokE,aacD,BSU_21638,BSU_21639,yokC,yokB,sprA,msrB,msrA,ypoP,dinF,ypmT,ypmS,ypmR,[scuA]
* * ÷ NC_000964 2652547 2677517 24971 13 [0] [1] 2 [nucB]–yqbO [nucB],spoIVCA,arsC,arsB,yqcK,arsR,yqcI,rapE,phrE,yqzI,rttG,rttF,BSU_25875,yqxJ,yqxI,cwlA,yqxH,yqxG,yqcE,yqcD,yqcC,yqcB,yqcA,yqbT,yqbS,yqbR,yqbQ,yqbP,yqbO
* * ÷ NC_000964 2677742 2725102 47361 2 [1] [1] 2 txpA–czcD txpA,bsrH,yqbM,yqbK,yqzN,yqbJ,yqbI,yqbH,yqbG,yqbF,yqbE,yqbD,yqbC,yqbB,yqbA,yqaT,yqaS,yqaR,yqaQ,yqaP,yqaO,yqaN,yqzO,sknM,yqaL,yqaK,yqaJ,yqaI,BSU_26305,sknH,yqaG,yqdA,yqaF,sknR,yqaD,yqaC,yqaB,BSU_26399,yrkS,psiE,yrkQ,yrkP,yrkO,BSU_26449,yrkN,yrkL,yrkK,yrkJ,yrkI,yrkH,yrkF,yrkE,yrkD,yrkC,BSU_26569,yrkB,bltR,blt,bltD,yrkA,yrzO,yrdR,czcR,yrdP,czcD
* * ÷ NC_000964 2725412 2750354 24943 2 [1] [0] 12 [yrdN]–[yraK] [yrdN],gltR,yrdK,brnQ,azlD,azlC,azlB,yrdF,cypA,yrdC,yrdB,yrdA,aadK,yrpB,murI,yrpD,yrpDX,BSU_26827,zinT,sigZ,yrpG,yraO,yraN,prpF,csn,yraL,[yraK]
* * ÷ NC_000964 3321387 3336218 14832 7 [0] [1] 2 lytH–[pucE] lytH,fisB,yunC,yunD,yunE,yunF,yunG,pucH,pucR,pucJ,pucK,pucL,pucM,yuzJ,auzJ,[pucE]
* * ÷ NC_000964 3336431 3344517 8087 2 [1] [1] 2 [pucD]–[bsnA] [pucD],pucC,pucB,pucA,pucG,pucF,BSU_32539,[bsnA]
* * ÷ NC_000964 3344876 3347255 2380 2 [1] [0] 2 [bsnA]–[frlD] [bsnA],frlP,frlR,[frlD]
* * ÷ NC_000964 3347421 3354935 7515 2 [0] [0] 7 [frlD]–glxB [frlD],frlM,frlN,frlO,frlB,yurQ,dadA,sspG,yurS,glxB
* * ÷ NC_000964 3362691 3362749 59 2 [1] [1] 2 metP methionine ABC transporter, permease component
* * ÷ NC_000964 3836067 3846607 10541 15 [0] [1] 2 [sboA]–[rapF] [sboA],sboX,albA,albB,albC,albD,albE,albF,albG,ywhL,ywhK,[rapF]
* * ÷ NC_000964 3846670 3847824 1155 2 [1] [0] 15 [rapF]–ywhH [rapF],phrF,ywhH
* * ÷ NC_000964 4049083 4056028 6946 2 [0] [1] 2 [pdp]–[yxeQ] [pdp],nupC,deoC,deoR,yxxB,yxeR,[yxeQ]
* * ÷ NC_000964 4056247 4060733 4487 2 [1] [1] 2 [yxeQ]–[snaB] [yxeQ],sndB,yxeO,yxeN,yxeM,[snaB]
* * ÷ NC_000964 4060889 4086181 25293 2 [1] [1] 2 [yxeK]–[iolS] [yxeK],yxeJ,yxeI,hadM,yxeG,yxeF,cotNE,yxeD,yxeC,frxB,yxeA,tpeM,tpeL,tpeK,tpeJ,iolJ,iolI,iolH,iolG,iolF,iolE,iolD,iolC,iolB,iolA,iolR,[iolS]
* * ÷ NC_000964 4086377 4121766 35390 2 [1] [0] 2 [iolS]–[bglA] [iolS],yxcE,yxcD,csbC,htpG,yxcA,yxbG,yxbF,aldX,yxbD,yxbC,yxbB,yxbA,yxnB,asnH,yxaM,yxaL,yxaJ,yxaI,yxaH,qdoI,qdoR,yxnA,yxaD,yxzK,yxaC,yxaB,glxK,gntR,gntK,gntP,gntZ,ahpC,ahpF,[bglA]
* * ÷ NC_000964 4121910 4131373 9464 2 [0] [0] 2 [bglA]–[yydD] [bglA],ptsJ,yydK,yyzN,liaM,liaL,liaK,liaE,liaD,fbp,[yydD]
* * ÷ NC_000964 4131539 4132688 1150 2 [0] [1] 2 [yydD]–[yydC] [yydD],[yydC]
* * ÷ NC_000964 4132770 4133270 501 2 [1] [0] 2 yydB putative phosphohydrolase
* * ÷ NC_000964 4133502 4147134 13633 2 [0] [0] 17 [yydB]–rocR [yydB],rlmH,yyzF,yycS,yycR,yyzG,yycQ,yycP,yycO,yycN,rapG,phrG,argI,rocE,rocD,rocR
* * ÷ NC_000964 4169166 4180062 10897 26 [0] [1] 2 [yybP]–[yybF] [yybP],yybO,BSU_40576,yybN,yybM,yybL,yybK,yybJ,ictI,yybH,yybG,[yybF]
* * ÷ NC_000964 4180164 4182102 1939 2 [1] [1] 2 [yybF]–[yybC] [yybF],yybE,yybD,[yybC]
* * ÷ NC_000964 4182174 4188065 5892 2 [1] [1] 2 [yybC]–[tetB] [yybC],yybB,yybA,yyaT,yyaS,yyaR,yyaQ,yyaP,[tetB]
* * ÷ NC_000964 4188200 4195483 7284 2 [1] [1] 3 [tetB]–[yyaJ] [tetB],tetL,yyaO,yyaN,yyaM,yyaL,yyaK,[yyaJ]
* * ÷ NC_000964 4195579 4195744 166 3 [1] [0] 26 yyaJ putative transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000964 = 2021270 (0.000)15 (0.970)
+AAA		 14/482 0.1 100% coding (2/141 nt) ybbU hypothetical protein
?NC_000964 220036 = 0 (0.000)intergenic (+4/‑243) skfH/ybdG sibling killing effect; sporulation killing factor biosynthesis and export/putative hydrolase/transferase
* ? NC_000964 = 4044570 (0.000)17 (1.090) 16/488 0.1 100% intergenic (+14/+1) srfT/ycxB surfactin exporter/hypothetical protein
?NC_000964 454596 = 0 (0.000)intergenic (+4/+56) sipU/yczH type I signal peptidase/putative hydrolase
* ? NC_000964 = 5295030 (0.000)12 (0.770) 11/488 0.3 100% intergenic (+81/+2) trnS‑Leu2/ydcL tRNA‑Leu/ICEBs1 mobile element: integrase
?NC_000964 634384 = 0 (0.000)intergenic (+522/‑726) gmuG/trnE‑Arg exported mannan endo‑1,4‑beta‑mannosidase/tRNA‑Arg
* ? NC_000964 = 7532641 (0.060)27 (1.730) 24/488 0.0 98.2% intergenic (+133/‑1) yeeI/cotK putative DNA integrase or transcriptional regulator/spore inner coat protein
?NC_000964 773981 = 0 (0.000)intergenic (+1/‑157) rhgX/yesY rhamnogalacturonan exolyase/rhamnogalacturonan acetylesterase
* ? NC_000964 = 11481540 (0.000)15 (0.960) 14/488 0.1 100% intergenic (+13/‑1) sbcC/hlpB DNA ATP‑dependent repair enzyme/HNH nuclease‑like essential for DNA repair
?NC_000964 1174096 = 0 (0.000)intergenic (+5/+40) slpS/yitE 2‑phospho‑3‑sulfolactate synthase/putative integral inner membrane protein
* ? NC_000964 = 12636670 (0.000)17 (1.090) 17/488 0.1 100% intergenic (+806/+35) trnSL‑Val2/yjcM tRNA‑Val/conserved hypothetical protein; phage island
?NC_000964 1279496 = 0 (0.000)intergenic (‑602/+18) yjdJ/ctaO hypothetical protein/minor protoheme IX farnesyltransferase 1 (heme O synthase)
* ? NC_000964 = 17821250 (0.000)8 (0.510) 8/488 0.5 100% coding (220/618 nt) pksA transcriptional regulator of bacillaene synthesis operon
?NC_000964 1863810 = 0 (0.000)coding (363/708 nt) pghC gamma‑polyglutamate hydrolase (phage origin)
* ? NC_000964 = 19601960 (0.000)7 (0.450) 7/486 0.6 100% intergenic (‑109/+2) yngL/ppsE putative integral inner membrane protein/nonribosomal plipastatin synthetase E
?NC_000964 1997969 = 0 (0.000)intergenic (‑12/+371) ppsA/dacC non‑ribosomal plipastatin synthetase A involved in synthesis of plipastatin/D‑alanyl‑D‑alanine carboxypeptidase
* ? NC_000964 = 20468390 (0.000)13 (0.840) 11/484 0.3 100% intergenic (+32/+141) yoaV/yoaW putative modified amino acid or TCA compound efflux permease/biofilm forming exported protein
?NC_000964 2079059 = 0 (0.000)intergenic (+433/+155) yobO/csaA putative phage‑related pre‑neck appendage protein; defective prophage 6/molecular chaperone for secreting proteins
* ? NC_000964 = 21460120 (0.000)11 (0.710) 11/486 0.3 100% coding (42/234 nt) yoyG putative toxin of a type I toxin family (sporulation operon)
?NC_000964 2292285 = 0 (0.000)intergenic (‑1/+147) scuA/ypmP assembly factor BSco of the Cu(A) site of cytochrome c oxidase/hypothetical protein
* ? NC_000964 = 26525460 (0.000)9 (0.720)
+48 bp		 9/392 0.2 100% coding (252/411 nt) nucB sporulation‑specific matrix degrading exported DNase
?NC_000964 2750355 = 0 (0.000)coding (696/816 nt) yraK putative hydrolase
* ? NC_000964 = 33213860 (0.000)7 (0.450) 7/486 0.6 100% intergenic (‑166/‑69) lipA/lytH lipoyl synthase (lipoic acid synthetase)/sporulation‑specific L‑Ala‑D‑Glu endopeptidase
?NC_000964 3354936 = 0 (0.000)intergenic (‑2/+109) glxB/yuzN methylglyoxalase; lactoylbacillithiol lyase/putative transcriptional regulator
* ? NC_000964 = 38360660 (0.000)15 (0.980) 15/478 0.1 100% coding (9/132 nt) sboA subtilosin A
?NC_000964 3847825 = 0 (0.000)intergenic (+4/+28) ywhH/speB putative tRNA editing enzyme/agmatinase
* ? NC_000964 = 40490592 (0.130)17 (1.090) 17/486 0.1 94.5% coding (1252/1302 nt) pdp pyrimidine‑nucleoside phosphorylase
?NC_000964 4147135 = 0 (0.000)intergenic (+3/+284) rocR/BSU_40358 transcriptional regulator (NtrC/NifA family)/hypothetical protein
* ? NC_000964 = 41691650 (0.000)26 (1.660) 26/488 0.0 100% intergenic (+32/+1) ppaC/yybP inorganic pyrophosphatase (Mn2+‑dependent)/putative manganese‑binding lipoprotein
?NC_000964 4195745 = 0 (0.000)intergenic (+1/+33) yyaJ/maa putative transporter/maltose O‑acetyltransferase