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Strain - 詳細 -

分譲依頼
株番号 NIES-2235
保存機関名 国立環境研究所
門名 Chlorophyta 綱名 Chlorophyceae
目名 科名
学名   Chlamydomonas reinhardtii P.A.Dangeard
シノニム Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941
一般名 緑藻; クラミドモナス; コナミドリムシ
地名(採集年月) 米国  マサチューセッツ  アマースト近郊 ()
採集者名  
生息環境(分離源)
生息環境の詳細   
分離者名(分離年月)
同定者名
株の状態 無菌[2018 Feb]; 単藻; クローン
保存形態 継代培養/凍結保存
凍結保存 不明
培養条件(前培養条件) 培地名:C (寒天)
培地形状:固体
温度:20 ℃
光強度:21 μmol phtons/m2/sec  明暗周期:10L:14D
継代培養周期:6 M
遺伝子情報 18S rRNA (AB701550 ) Amt protein mRNA(putative) (AF509497 ) CAH1 (AB026126 ) CAH1 (D90206 ) CAH2 (X54488 ) CrDES (AB239769 ) EST information (http://est.kazusa.or.jp/en/plant/chlamy/EST/index.html ) GCS1 (AB206813 ) mmp1 (AB058413 ) mmp1 (AB058411 ) mmp2 (AB058412 ) mmp2 (AB058414 ) mmp3 (AB108850 ) NSG1 (AB167472 ) NSG11 (AB167476 ) NSG13 (AB167477 ) NSG17 (AB167478 ) NSG6 (AB167474 ) NSG7 (AB167475 ) RECA (AB048829 ) SIG1 mRNA (AB049220 ) Total genome information (http://chlamy.pmb.lif.kyoto-u.ac.jp/chlamybase )
画像
特性
  走光性; デンプン生産 (Kuchitsu et al. 1988)  ゲノム解読株; 交配型(−); NIES-2239 (IAM C-562)と交配; 餌料 (Liu et al. 2016)
細胞サイズ(min-max)   体制 単細胞性; 鞭毛性  
注意事項 凍結保存[2018 Nov]; 無菌  

文献
Ban, S.: 1992: Effects of photoperiod, temperature, and population density on induction of diapause egg production in Eurytemora affinis (Copepoda: Calanoida) in Lake Ohnuma, Hokkaido, Japan.: J. Crustacean Biol., 12, 361-367.
Strains: 282 2235

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T.: 1984: Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing.: Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strains: 18 19 21 24 26 29 31 39 40 41 44 45 46 50 73 74 75 76 77 80 81 87 88 89 90 91 93 95 98 99 100 101 120 122 131 135 137 144 151 204 207 213 257 263 379 415 2219 2235 2239 2579

Tsuzuki, M.: 1983: Mode of HCO3-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air.: Z. Pflanzenphysiol., 110, 29-37.
Strains: 2160 2170 2235

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S.: 1984: Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants.: Ann. NY Acad. Sci., 429, 238-240.
Strains: 687 2160 2161 2162 2170 2173 2235 2280

Miyachi, S., Tsuzuki, M., Aizawa, K.: 1985: Factors which enhance photosynthesis in microalgae under limiting CO2 concentrations.: In Kinetics of Photosynthetic Carbon Metabolism in C3-plants. II. Electron Transport, Transport of CO2, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, pp. 88-92.
Strains: 2160 2235

Yang, S.-Y., Tsuzuki, M., Miyachi, S.: 1985: Carbonic anhydrase of Chlamydomonas: Pulification and studies on its induction using antiserum against Chlamydomonas carbonic anhydrase.: Plant Cell Physiol., 26, 25-34.
Strains: 2235

Toguri, T., Muto, S., Miyachi, S.: 1986: Biosynthesis and intracellular processing of carbonic anhydrase in Chlamydomonas reinhardtii.: Eur. J. Biochem., 58, 443-450.
Strains: 2235

Aizawa, K. & Miyachi, S.: 1986: Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria.: FEMS Microbiol. Lett., 39, 215-233.
Strains: 2093 2160 2173 2235

Yagawa, Y., Aizawa, K., Yang, S.-Y., Miyachi, S.: 1986: Release of carbonic anhydrase from the cell surface of Chlamydomonas reinhardtii by trypsin.: Plant Cell Physiol., 27, 215-221.
Strains: 2235

Kuchitsu, K., Tsuzuki, M., Miyachi, S.: 1988: Changes of starch localization within the chloroplast induced by changes in CO2 concentration during growth of Chlamydomonas reinhardtii. Independent regulation of pyrenoid starch and stroma starch.: Plant Cell Physiol., 29, 1269-1278.
Strains: 2235

Kuchitsu, K., Tsuzuki, M., Miyachi, S.: 1988: Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonas reinhardtii. Particulate form of RuBisCO protein.: Protoplasma, 144, 17-24.
Strains: 2235

Yagawa, Y., Muto, S., Miyachi, S.: 1988: Carbonic anhydrase of Chlamydomonas reinhardtii. Cellular distribution and subunit constitution in two strains.: Plant Cell Physiol., 29, 185-188.
Strains: 2235

Bürger, J., Miyachi, S., Galland, P., Senger, H.: 1988: Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO2-conditions.: Botanica Acta, 101, 229-232.
Strains: 2173 2235

Tsuzuki, M. & Miyachi, S.: 1989: The function of carbonic anhydrase in aquatic photosynthesis.: Aquatic Botany, 34, 85-104.
Strains: 2093 2138 2160 2173 2235

Dionisio, M. L., Tsuzuki, M., Miyachi, S.: 1989: Light requirement for carbonic anhydrase induction in Chlamydomonas reinhardtii.: Plant Cell Physiol., 30, 207-213.
Strains: 2235

Dionisio, M. L., Tsuzuki, M., Miyachi, S.: 1989: Blue light induction of carbonic anhydrase activity in Chlamydomonas reinhardtii.: Plant Cell Physiol., 30, 215-219.
Strains: 2235

Toguri, T., Muto, S., Mihara, S., Miyachi, S.: 1989: Synthesis and degradation of carbonic anhydrase in a synchronized culture of Chlamydomonas reinhardtii.: Plant Cell Physiol., 30, 533-539.
Strains: 2235

Ehara, T., Ogasawara, Y., Osafune, T.: 1990: Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii (Chlorophyta) in synchronized culture.: J. Phycol., 26, 317-323.
Strains: 2235

Kashino, Y., Enami, I., Satoh, K., Katoh, S.: 1990: Immunological cross-reactivity among corresponding proteins of photosystems I and II from widely divergent photosynthetic organisms.: Plant Cell Physiol., 31, 479-488.
Strains: 2093 2138 2235

Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A.: 1990: Effects of CO2 concentration during growth on fatty acid composition in microalgae.: Plant Physiol., 93, 851-856.
Strains: 48 2093 2138 2160 2235

Fukuzawa, H., Fujiwara, S., Yamamoto, Y., Dionisio-Sese, M. L., Miyachi, S.: 1990: cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: Regulation by environmental CO2 concentration.: Proc. Nati. Acad. Sci. U. S. A., 87, 4383-4387.
Strains: 2235

Mihara, S. & Hase, E.: 1971: Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. I. Some characteristics of the cell cycle.: Plant Cell Physiol., 12, 225-236.
Strains: 2235

Mihara, S. & Hase, E.: 1971: Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. II. Effects of chloramphenicol and cycloheximide on the length of cell cycle.: Plant Cell Physiol., 12, 237-241.
Strains: 2235

Osafune, T., Mihara, S., Hase, E., Ohkuro, I.: 1972: Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. II. Association of mitochondria and the chloroplast at an early developmental stage.: Plant Cell Physiol., 13, 981-989.
Strains: 2235

Osafune, T., Mihara, S., Hase, E., Ohkuro, I.: 1972: Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. I. Some characteristics of changes in subcellular structures during the cell cycle, especially in formation of giant mitochondria.: Plant Cell Physiol., 13, 211-227.
Strains: 2235

Osafune, T., Mihara, S., Hase, E., Ohkuro, I.: 1975: Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. III. Three-dimensional structures of mitochondria in the cells at intermediate stages of the growth phase of the cell cycle.: J. Electr. Microsc., 24, 247-252.
Strains: 2235

Mihara, S. & Hase, E.: 1975: Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. III. Some notes on the process of zoospore liberation.: Plant Cell Physiol., 16, 371-375.
Strains: 2235

Mihara, S. & Hase, E.: 1975: Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. IV. Effects of 6-methyl purine on the revolution of the cell cycle.: Plant Cell Physiol., 16, 1121-1130.
Strains: 2235

Osafune, T., Mihara, S., Hase, E., Ohkuro, I.: 1976: Electron microscope studies of the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. IV. Mitochondria in dividing cells. : J. Electr. Microsc., 25, 261-269.
Strains: 2235

Mihara, S. & Hase, E.: 1978: Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. V. The transition point for the effect of 6-methyl purine in the cell cycle.: Plant Cell Physiol., 19, 83-89.
Strains: 2235

Tsubo, Y. & Matsuda, Y.: 1984: Transmission of chloroplast genes in crosses between Chlamydomonas reinhardtii diploids: Correlation with chloroplast nucleoid behavior in young zygotes.: Curr. Genet., 8, 223-229.
Strains: 2235 2239

Yamano, T., Miura, K., Fukuzawa, H.: 2008: Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii.: Plant Physiol., 147, 340-354.
Strains: 2235

Kajikawa, M., Yamato, K. T., Kohzu, Y., Shoji, S., Sakai, Y., Fukuzawa, H.: 2006: A front-end desaturase from the green alga Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by ω13 desaturation in methylotrophic yeast and tobacco.: Plant Cell Physiol., 47, 64-73.
Strains: 2235

Yoshioka, S., Taniguchi, F., Miura, K., Inoue, T., Yamano, T., Fukuzawa, H.: 2004: The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii.: Plant Cell, 16, 1466-1477.
Strains: 2235

Miura, K., Yamano, T., Yoshioka, S., Kohinata, T., Inoue, Y., Taniguchi, F., Asamizu, E., Nakamura, Y., Tabata, S., Yamato, K. T., Ohyama, K., Fukuzawa, H.: 2004: Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii.: Plant Physiol., 135, 1595-1607.
Strains: 2235

Asamizu, E., Nakamura, Y., Miura, K., Fukuzawa, H., Fujiwara, S., Hirono, M., Iwamoto, K., Matsuda, Y., Minagawa, J., Shimogawara, K., Takahashi, Y., Tabata, S.: 2004: Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta), to facilitate gene function analysis.: Phycologia, 43, 722-726.
Strains: 2235

Abe, J., Kubo, T., Takagi, Y., Saito, T., Miura, K., Fukuzawa, H., Matsuda, Y.: 2004: The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii.: Curr. Genet., 46, 304-315.
Strains: 2235

Kubo, T., Saito, T., Fukuzawa, H., Matsuda, Y.: 2001: Two tandemly-located matrix metalloprotease genes with different expression patterns in the Chlamydomonas sexual cell cycle.: Curr. Genet., 40, 136-143.
Strains: 2235

Asamizu, E., Miura, K., Kucho, K., Inoue, Y., Fukuzawa, H., Ohyama, K., Nakamura, Y., Tabata, S.: 2000: Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii.: DNA Res., 7, 305-307.
Strains: 2235

Asamizu, E., Nakamura, Y., Sato, S., Fukuzawa, H., Tabata, S.: 1999: A large scale structure analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags.: DNA Res., 6, 369-373.
Strains: 2235

Tachiki, A., Fukuzawa, H., Miyachi, S.: 1992: Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii.: Biosci. Biotechnol. Biochem., 56, 794-798.
Strains: 2235

Fukuzawa, H., Ishida, S., Miyachi, S.: 1991: cDNA cloning and gene expression of carbonic anhydrase in Chlamydomonas reinhardtii.: Can. J. Bot., 69, 1088-1096.
Strains: 2235

Fujiwara, S., Fukuzawa, H., Tachiki, A., Miyachi, S.: 1990: Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii.: Proc. Nati. Acad. Sci. U. S. A., 87, 9779-9783.
Strains: 2235

Kamo, T., Shimogawara, K., Fukuzawa, H., Muto, S., Miyachi, S.: 1990: Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii.: Eur. J. Biochem., 192, 557-562.
Strains: 2235

Dionisio-Sese, M. L., Fukuzawa, H., Miyachi, S.: 1990: Light-induced carbonic anhydrase expression in Chlamydomonas reinhardtii.: Plant Physiol., 94, 1103-1110.
Strains: 2235

Kubo, T., Abe, J.,  Saito, T., Matsuda, Y.: 2002: Genealogical relationships among laboratory strains of Chlamydomonas reinhardtii as inferred from matrix metalloprotease genes.: Curr. Genet., 41, 115-122.
Strains: 2235

Fujii, K., Nakashima, H., Hashidzume, Y.: 2010: Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan.: J. Appl. Microbiol., 108, 1421-1429.
Strains: 2172 2235 2848 2849 2850 2851

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M.: 2012: Isolation of herbicide-resistant mutants of Botryococcus braunii.: Bioresour. Technol., 109, 300-303.
Strains: 35 119 227 230 2235

Yumoto, K., Kasai, F., Kawachi, M.: 2013: Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection.: Microbiol. Cult. Coll., 29, 1-12.
Strains: 158 437 438 440 884 968 1021 1022 1048 1733 1848 1849 2201 2202 2203 2204 2206 2207 2208 2209 2210 2211 2212 2213 2214 2215 2216 2218 2219 2220 2222 2223 2224 2225 2228 2229 2230 2231 2233 2234 2235 2236 2237 2238 2239 2240 2241 2242 2243 2246 2247 2248 2314 2315 2316 2317 2318 2319 2320 2321 2322 2323 2324 2462 2463 2499 2575 2576 2577 2578 2579 3284

Yamano, T., Iguchi, H. Fukuzawa, H.: 2013: Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.: J. Biosci. Bioeng., 115, 691-694.
Strains: 2235

Wang, L., Yamano, T., Kajikawa, M., Hirono, M., Fukuzawa, H.: 2014: Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii.: Photosynth. Res., 121, 175-184.
Strains: 2235

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S.: 2015: Combined effects of temperature and food concentration on growth and reproduction of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan).: Freshw. Biol., 60, 2003-2018.
Strains: 282 2235

Kong, F., Yamasaki, T., Kurniasih, S. D., Hou, L., Li, X., Ivanova, N., Okada, S., Ohama, T.: 2015: Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains.: Biotech. Bioeng., 120, 239-245.
Strains: 2235

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S.: 2014: Effects of temperature on life history traits of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan).: Limnology, 15, 85-97.
Strains: 282 2235

Liu, X., Syuhei, B.: 2017: Effects of acclimatization on metabolic plasticity of Eodiaptomus japonicus (Copepoda: Calanoida) determined using an optical oxygen meter.: J. Plankton Res., 39, 111-121.
Strains: 282 2235

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N.: 2017: Characterization of phosphoethanolamine-N-methyltransferases in green algae.: Biochem. Biophys. Res. Commun., 488, 141-146.
Strains: 2202 2207 2212 2235 2242

Ishinishi, R., Matsuura, H., Tanaka, S., Nozawa, S., Tanada, K., Kawashita, N., Fujiyama, K., Miyasaka, H., Hirata, K.: 2018: Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga.: Gene, 640, 14-20.
Strains: 2235

Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G.: 2018: Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis.: eLife, 7, e35122 (article ID).
Strains: 2166 2235 2541

Toyama, T., Kasuya, M., Hanaoka, T., Kobayashi, N., Tanaka, Y., Inoue, D., Sei, K., Morikawa, M., Mori, K.: 2018: Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater efuent.: Biotechnol. Biofuels, 11, 176 (article ID).
Strains: 48 2172 2235

Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M.: 2018: Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.—: Microb. Resour. Syst., 34, 73-82.
Strains: 18 35 242 691 735 1271 1278 2185 2187 2235 2261 2540 2572 3983

Seto, K. & Degawa, Y.: 2018: Collimyces mutans gen. et sp. nov. (Rhizophydiales, Collimycetaceae fam. nov.), a new chytrid parasite of Microglena (Volvocales, clade Monadinia).: Protist, 169, 507-520.
Strains: 755 858 2220 2235 2743 2744

Yamano, T. & Fukuzawa, H.: 2020: Transformation of the model microalga Chlamydomonas reinhardtii without cell-wall removal.: In Electroporation Protocols. Methods in Molecular Biology, Eds. by Li, S., Chang, L., Teissie, J., Humana, New York, NY, 2050, pp. 155-161.
Strains: 2235

Hang, L. T., Mori, K., Tanaka, Y., Morikawa, M., Toyama, T.: 2020: Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl2 stresses.: Bioprocess. Biosyst. Eng., 43, 971–980.
Strains: 2235

Nakanishi, A., Iritani, K., Sakihama, Y., Ozawa, N., Mochizuki, A., Watanabe, M.: 2020: Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer.: AMB Express, 10, 112 (article ID).
Strains: 2235

Nakanishi, A., Iritani, K., Sakihama, Y., Watanabe, M., Mochiduki, A., Tsuruta, A., Sakamoto, S., Ota, A.: 2021: Fabrication and biodegradability of starch cell-plastics as recyclable resources.: Appl. Sci., 11, 847 (article ID).
Strains: 2235

Yamano, T., Toyokawa, C., Shimamura, D., Matsuoka, T., Fukuzawa, H.: 2021: CO2-dependent migration and relocation of LCIB, a pyrenoid-peripheral protein in Chlamydomonas reinhardtii.: Plant Physiol., 188, 1081–1094.
Strains: 2235

Nakanishi, A., Iritani, K., Tsuruta, A., Yamamoto, N., Watanabe, M., Ozawa, N., Watanabe, M., Zhang, K., Tokudome, A.: 2022: Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material.: Photosynth. Res., 106, 4459–4468.
Strains: 2235

Hang, L. T., Mori, K., Toyama, T.: 2020: Enhanced biomass and lipid production capacity of Chlamydomonas reinhardtii under mixotrophic cultivation using sewage effluent and waste molasses.: Jpn. J. Water Treat. Biol., 56, 57-66.
Strains: 2235

Nakanishi, A., Nemoto, S., Yamamoto, N., Iritani, K., Watanabe, M.: 2023: Identification of cell-attachment factors derived from green algal cells disrupted by sonication in fabrication of cell plastics.: Bioengineering-Basel, 10, 893 (article ID).
Strains: 2235

Nakanishi, A., Omino, N., Nakamura, T., Goto, S., Matsumoto, R., Yomogita, M., Narisawa, N., Kimijima, M., Iritani, K.: 2024: Evaluation of cellular responses of heterotrophic Escherichia coli cultured with autotrophic Chlamydomonas reinhardtii as a nutrient source by analyses based on microbiology and transcriptome.: Microorganisms, 12, 452 (article ID).
Strains: 2235