Studies on breeding of dominant nuclear dwarf male sterile lines with a blue seed marker in common wheat

Zong-Jun Pu^1,2, Ze-Hong Yan², Wu-Yun Yang¹ and You-Liang Zheng²

¹Crop Research Institute, Sichuan Academy of Agriculture Science Chenghu, 610066, China
²Thticeae Research Institute, Sichuan Agricultural University Dujiangyan 611830, China

Summary

The genotype 89-2343 of durum wheat with Ms2 and a blue seed marker was crossed and backcrossed with a common wheat genotype 7739-3 to produce male fertile plants with blue seeds (MFP-BS). To combine the blue seed marker, dwarf male sterile plants carrying Rht10 and Ms2 were fertilized with pollen from selected MFP BS. At last, the combination of blue seed marker, Ms2 and Rht10 was successfully produced. The segregation ratio of male sterility, seed color as well as chromosome configurations of the combinations suggested that the blue seed marker, Ms2 and Rht10were located on the same chromosome. Cytological analysis indicated that the male sterile wheat line with a blue seed marker was 43 in chromosome number, with an additional chromosome. The transmission rate for blue seed male sterile plants was 22.1%. In addition, the potential value for blue marker sterile lines in wheat breeding and hybrid production is discussed.

Key words: Triticum aestivum, blue seed marker, dwarf male sterile line, cytological analysis, hybrid production