7. AFLP amplified fragment length polymorphism
A nomenclature proposal for AFLP loci has been received from Marc Zabeau at
Keygene with the format 'XxyzAN1N2N3, where 'X' is the usual
symbol for a DNA marker of unknown function; 'xyz' is the usual laboratory designator
(e.g., kg for Keygene); A is a single upper-case letter denoting the
rare-cutter enzyme used, e.g., P for PstI, etc.; N1 and N2 are two-digit
numbers identifying standard one, two or three base-pair extensions (standard
lists will be provided by Keygene); and N3 is a three-digit number corresponding
to the molecular weight of the fragment.
The foregoing should be considered only as a proposal at this time as no AFLPs
are listed in the catalogue. Comments regarding the proposal are welcomed
and should be sent to the authors.
8. Guidelines for Nomenclature of Genes for Reaction to Pathogenic Diseases
and Pest
1. All genes for resistance (low reaction) will be designated with a capital
letter, even though they behave as recessive alleles. Moreover, the dominance
of individual alleles may vary with the environment, the genetic background
and the particular culture of the pathogen. Symbols for disease/pest-reaction
genes are used by people of many disciplines, and since they are frequently
communicated verbally, dominance relationships are not clear. Those alleles
initially designated with a lower-case letter have tended to be miswritten with
a capital. For example, the usually recessive resistance allele Sr17
was initially designated sr17 but its presentation in some reports was
confusing.
2. Where no recombination occurs between genes conferring resistance to more
than one pathogen, the gene(s) segment shall be designated separately for each
disease; e.g. Pm1, Sr15 and Lr20.
3. Where recombination I>occurs between two closely linked factors for reaction
to a pathogen, the recombined 'allele' may be designated as a combination of
the separate alleles; e.g. the recombined 'allele' obtained by combining Lr14a
and Lr14b was designated as Lr14ab. The decision as to whether
a designation should be as a combination or as separate genes shall be at the
discretion of particular workers. A maximum value of 1 crossover unit for designation
as an 'allele' is suggested. Although the need to consider uniform symbolization
of corresponding genes in pathogens is recognized, no recommendations are proposed.