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11. DNA Markers
See 'Genetic nomenclature proposal' above for a proposal for the naming of AFLP
loci. The following list catalogues DNA-marker loci that (1) have been detected
either by Southern hybridization of DNA restriction fragments or as sequence-tagged-sites
by amplification of DNA fragments with primers and (2) have been localized to
specific wheat chromosomes. The formal listings of the 5S-RNA or 18S-5.8S-26S
rRNA (Nor) loci are included elsewhere in the catalogue. No attempt has been made
to list orthologous loci in related species, although many have been identified
{e.g., 1329,1330}. In addition we list genes that appear on consensus maps prepared
by Dr R. Appels and various colleagues. The nomenclature used is that originally
published in the 1994 Supplement, except for some loci detected with 'known-function'
clones for which other nomenclature has been used in the publications cited. The
reference(s) that follow the locusp? symbols designate the publication(s) in which
the chromosomal locations or map positions of the loci were first reported. References
that are in parentheses { } contain the listed locus symbol. Temporary symbols
for a few DNA markers detected with known-function DNA probes are marked with
an asterisk, *, ; these are temporary, pending assignment of the laboratory designator.
Synonyms are listed in parentheses [] in the second column. Where symbols were
assigned by the curators to comply with nomenclature guidelines the same reference
numbers follow the gene symbol and the synonym. Other chromosomes bearing markers
detected with the same probe or the same primers are indicated in parentheses
after the probe or the primers. To permit flexibility in using the database, each
homoeologous group is bracketed separately. Three revisions were made in the organization
of the DNA Markers section, as follows:
1.Markers in homoeologous chromosome groups 4, 5 and 7 (with the exception of
those in T. monococcum chromosome 4Am; see #2 below) are listed
in groups composed of loci located in homoeologous segments. The groups include
the six classical homoeologous arm groups, namely, 4S (4AL:4BS:4DS), 4L (4AS:4BL:4DL),
5S (5AS:5BS:5DS), 5L (5AL:5BL:5DL), 7S (7AS:7BS:7DS) and 7L (7AL:7BL:7DL), and
five new groups, 4AL:4BL:4DL, 5AL:4BL:4DL, 4AL:5BL:5DL, 7BS:5BL:7DS, and 7AS:4AL:7DS.
Evidence is not available regarding the correct group location for a few of the
markers listed in groups 4S, 4L, and 7S; a double asterisk (**) after the locus
reference identifies these markers.
2.Markers in T. monococcum 4Am are listed separately (under
4AmS, 4AmL, or 4Am), due to the several rearrangements
that distinguish 4A and 4Am.
3 .Superscripts appended to locus references designate the species in which loci
were analyzed, as follows:
'1' T. aestivum,
'2' T. turgidum,
'3' T monococcum,
'4' Ae. tauschii, and
'5' Species hybrid,
with the exception that the superscript is omitted for markers studied only in
T. aestivum.
a' Designates primer pairs that identify loci that cap the genetic
maps. The forward primer is a degenerate telomeric sequence and the reverse
primer is a specific sequence. Each primer combination identified multiple loci;
however, only telomeric (Tel) loci are included {888}.
b' Designates loci detected by hybridization with DNA clones whose sequences
are largely homologous with known gene in the EMBL database (1392).
STS's from RFLP clones: Certain STS markers are listed using sequences from
previously listed RFLP clones. The convention adopted is to add a 'p' to the
laboratory designneator. The 'References' to PCR markers refer, however, to the
paper(s) which reported the first chromosomal location detected by this PCR
marker.
Order of presentation: Gene, synonym, map location (approximate
distance in cM from the terminal end of the short arm), probe, all known locations
in homoeologous groups. In the output files genes appear in alphabetical order
with locus numbers in ascending order.
(Editorial remarks)
This article is a partial citation from the report by Dr. R. A. McIntosh at
the 10th International Wheat Genetics Symposium (Paestum, Italy).
The citation was allowed by the author as well as Dr. N. E. Pogna, Chairperson
of Local Organization Committee. The full text and the contents are available
in Proc. 10th IWGS (edited by N. E. Pogna et al; Istituto Sperimentale per la
Cerealicoltura, Via Cassia 176, 00191 Roma, Italy: http:// www.cerealicoltura.it),
or through data base KOMUGI ( http://www.shigen.nig.ac.jp/wheat/genecalog/macgene/
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